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01-11-2012 | Original Paper

Assessment of an asymmetrical coating stent with sirolimus released from ablumial matrix in porcine model

Authors: Li Shen, Yizhe Wu, Feng Zhang, Lingxiang Wu, Chao Dong, Yongxing Gao, Aijun Sun, Yunzeng Zou, Juying Qian, Jianguo Sun, Wei Zhong, Junbo Ge

Published in: Clinical Research in Cardiology | Issue 11/2012

Abstract

Background

Delayed endothelialization contributes to stent thrombosis of current drug-eluting stents. The asymmetrical coating technique provides an anti-proliferative effect abluminally without affecting luminal endothelialization. Layer-by-layer self-assembled chitosan/heparin (C/H LBL) has been proved to promote re-endothelialization. A novel stent system, C/H LBL coated luminally and sirolimus released abluminally (C/H LBL-SES), was fabricated.

Methods

Bare metal stents (BMS), traditionally circumferential sirolimus-eluting stents (SES), and C/H LBL-SES were implanted into porcine coronary arteries. At the 7, 14 and 28 days follow-up (FU), angiography, intravascular ultrasound (IVUS), vasomotor function induced by acetylcholine (Ach), scanning-electron microscopy and histopathology were performed. Remodeling index (RI) was based on IVUS and defined as cross-sectional area (CSA) of vessel at in-stent segment divided by CSA of reference vessel and expressed as a percentage with a normal range from 0.95 to 1.05.

Results

Thirty-eight mini pigs were enrolled and 74 stents (BMS = 23, C/H LBL = 28, SES = 23) were implanted in this study. At 28 days after implantation, the diameter stenosis of C/H LBL-SES by quantitative coronary angiography was 18.8 ± 2.5 %, the area stenosis by histomorphometry was 24.2 ± 2.9 %, which were comparable to that of SES and superior to BMS. At 14 days, re-endothelialization of C/H LBL-SES was almost completed, while only about 50 % of surface of SES was covered by endothelium. At 7, 14 and 28 days FU, although C/H LBL-SES suffered a greater vasoconstriction induced by Ach infusion than BMS (P < 0.05), it behaved better than SES (P < 0.01). No sign of stent malapposition was detected, while RI was within the normal range by IVUS. No acute or subacute thrombotic events occurred in all three groups.

Conclusions

The asymmetrically designed C/H LBL-SES successfully inhibited neointima hyperplasia, while diminishing vasoconstriction after Ach-stress. Endothelialization of C/H LBL-SES was less affected compared with traditionally circumferentially coated SES.

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Title: Assessment of an asymmetrical coating stent with sirolimus released from ablumial matrix in porcine model
Authors: Li Shen
Yizhe Wu
Feng Zhang
Lingxiang Wu
Chao Dong
Yongxing Gao
Aijun Sun
Yunzeng Zou
Juying Qian
Jianguo Sun
Wei Zhong
Junbo Ge
Publication date: 01-11-2012
Publisher: Springer-Verlag
Published in: Clinical Research in Cardiology / Issue 11/2012
Print ISSN: 1861-0684
Electronic ISSN: 1861-0692
DOI: https://doi.org/10.1007/s00392-012-0476-7

2024 ASCO Annual Meeting

Springer Medicine

Assessment of an asymmetrical coating stent with sirolimus released from ablumial matrix in porcine model

Abstract

Background

Methods

Results

Conclusions

2024 ASCO Annual Meeting

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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