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Comparative Clinical Pathology
Published in: Comparative Clinical Pathology 5/2014

01-09-2014 | Original Article

Assessment of a single-nucleotide polymorphism in specific protein 1 (SP1)-binding site of the Lactoferrin promoter region as potential genetic markers for uterine infection in dairy cows

Authors: Fahimeh Pourzal, Hassan Sharifiyazdi, Abdolah Mirzaei

Published in: Comparative Clinical Pathology | Issue 5/2014

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Abstract

The existence of single-nucleotide polymorphism (SNP) in specific protein 1 (SP1)-binding site of the lactoferrin gene was assessed using RFLP-PCR. Also, the relationship between this SNP and uterine infection and some reproductive parameters in dairy cows were investigated. Blood samples were collected from multiparous Holstein-Friesian dairy cows with a history of uterine infection (n = 30) and cows without uterine infection (n = 28), as control group. Using RFLP-PCR, we detected an SNP (G to A: position −190) within the SP1-binding site in the lactoferrin promoter region in cows with and without uterine infection; however, the results showed that higher percentage of cows with mutation had a history of uterine infection compared to that of cows without mutation (71.4 versus 28.6 %; P < 0.05). Cows with mutation need more service per conception and had higher days open compared to that of cow without mutation; however, this difference was not significant. These findings show that the mutation in SP1-binding site may be responsible for expression of the lactoferrin gene, therefore, it can be considered as a useful genetic marker for resistance to postpartum uterine infection in dairy cattle.
Literature
Baker EN, Anderson BF, Baker HM, Day CL, Hairdas M, Noris GE, Rumball SV, Smith CA, Thomas DH (1994) Three-dimensional structure of lactoferrin in various functional state. Adv Exp Med Biol 357:1–12PubMedCrossRef
Beaulieu E, Green L, Elsby L, Aloiurfi Z, Morand EF, Ray DW, Donn R (2011) Identification of a novel cell type-specific intronic enhancer of macrophage migration inhibitory factor (MIF) and its regulation by mithramycin. Clin Exp Immunol 163(2):178–188PubMedCrossRefPubMedCentral
Bouwman P, Philipsen S (2002) Regulation of the activity of SP1-related transcription factors. Mol Cell Endocrinol 195(1–2):27–38PubMedCrossRef
Buchanan DL, Setiawan T, Lubahn DB, Taylor JA, Kurita T, Cunha GR, Cooke PS (1999) Tissue compartment-specific estrogen receptor-alpha participation in the mouse uterine epithelial secretory response. Endocrinology 140(1):484–491PubMed
Cohen RO, Bernstein M, Ziv G (1995) Isolation and antimicrobial susceptibility of Actinomyces pyogenes recovered from the uterus of dairy with retained fetal membranes and postparturent endometritis. Theriogenology 43:1389–1397CrossRef
Cormican P, Meade K, Cahalane S, Narciandi F, Chapwanya A, Lloyd A, O’Farrelly C (2008) Evolution, expression and effectiveness in a cluster of novel bovine β-defensins. Immunogenetics 60(3):147–156PubMedCrossRef
Daly M, Ross P, Giblin L, Buckley F (2006) Polymorphisms within the lactoferrin gene promoter in various cattle breeds. Anim Biotechnol 17(1):33–42PubMedCrossRef
Green MP, Ledgard AM, Beaumont SE, Berg MC, McNatty KP, Peterson AJ, Back PJ (2011) Long-term alteration of follicular steroid concentrations in relation to subclinical endometritis in postpartum dairy cows. J Anim Sci 89(11):3551–3560PubMedCrossRef
Huang J, Wang H, Wang C, Li J, Li Q, Hou M, Zhong J (2009) Single nucleotide polymorphisms, haplotypes and combined genotypes of lactoferrin gene and their associations with mastitis in Chinese Holstein cattle. Mol Biol Rep 37(1):477–483PubMedCrossRef
Infantino V, Convertini P, Lacobazzi F, Pisano I, Scarcia P, Lacobazzi V (2011) Identification of a novel Sp1 splice variant as a strong transcriptional activator. Biochem Biophys Res Commun 412(1):86–91PubMedCrossRef
Inoue M, Yamada J, Kitamura N, Shimazaki K, Andren A, Ans Yamashita T (1993) Immunohistochemical localization of lactoferrin in bovine exocrine glands. Tissue Cell 25(5):791–797PubMedCrossRef
Ishibashi T, Yokura Y, Ohashi K, Yamamoto H, Maeda M (2011) Conserved GC-boxes, E-box and GATA motif are essential for GATA-4 gene expression in P19CL6 cells. Biochem Biophys Res Commun 413(2):171–175PubMedCrossRef
Jin H, Evangelou E, Ioannidis JPA, Ralston SH (2011) Polymorphisms in the 5′ flank of COL1A1 gene and osteoporosis: meta-analysis of published studies. Osteoporos Int 22(3):911–921PubMedCrossRef
Kathiravan P, Kataria RS, Mishra BP (2009) Single nucleotide polymorphism (SNP) identification and sequence analysis of 5′ flanking region of lactoferrin gene in Indian buffaloes (Bubalus bubalis). Livest Sci 121(1):38–44CrossRef
LeBlanc SJ (2008) Postpartum uterine disease and dairy herd reproductive performance: a review. Vet J 176:102–114PubMedCrossRef
Mann V, Hobson EE, Li B, Stewart TL, Grant SF, Robins SP, Aspden RM, Ralston SH (2001) A COL1A1 Sp1 binding site polymorphism predisposes to osteoporotic fracture by affecting bone density and quality. J Clin Invest 107(7):899–907PubMedCrossRefPubMedCentral
O‘Halloran F, Bahar B, Buckley F, O’Sullivan O, Sweeney T, Giblin L (2009) Characterisation of single nucleotide polymorphisms identified in the bovine lactoferrin gene sequences across a range of dairy cow breeds. Biochimie 91(1):68–75PubMedCrossRef
Plontzke J, Madoz LV, De la Sota RL, Heuwieser W, Drillich M (2011) Prevalence of clinical endometritis and its impact on reproductive performance in grazing dairy cattle in Argentina. Reprod Domest Anim 46(3):520–526PubMedCrossRef
Rojvirat P, Chavalit T, Muangsawat S, Thonpho A, Jitrapakdee S (2011) Functional characterization of the proximal promoter of the murine pyruvate carboxylase gene in hepatocytes: role of multiple GC boxes. Biochim Biophys Acta 1809(10):541–548PubMedCrossRef
Seyfert HM, Tuckoricz A, Interthal H, Koczan D, Hobom G (1994) Structure of the bovine lactoferrin-encoding gene and its promoter. Gene 143(2):265–269PubMedCrossRef
Sheldon IM, Cronin J, Goetze L, Donofrio G, Schuberth H (2009) Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle. Biol Reprod 81(6):1025–1032PubMedCrossRefPubMedCentral
Sheldon IM, Cronin J, Borges A (2011) The postpartum period and modern dairy cow fertility Part 1: uterine function. Livestock 16(4):14–18CrossRef
Thanseem I, Anitha A, Nakamura K, Suda S, Iwata K, Matsuzaki H, Ohtsubo M, Ueki T, Katayama T, Iwata Y, Suzuki K, Minoshima S, Mori N (2012) Elevated transcription factor specificity protein 1 in autistic brains alters the expression of autism candidate genes. Biol Psychiatry 71(5):410–418PubMedCrossRef
Turner ML, Healey GD, Sheldon IM (2012) Immunity and inflammation in the uterus. Reprod Domest Anim 47:402–409PubMedCrossRef
Valadan R, Mirzaei A, Sharifiyazdi H, Rowshan Ghasrodashti A, Safari H (2011) Detection of single nucleotide polymorphism in TATA box of lactoferrin gene and its association with uterine infections in cows. Online J Vet Res 15(3):190–199
Yanihara A, Mitsukawa K, Iwasaki S, Otsuki K, Kawamura T, Okai T (2007) High concentrations of lactoferrin in the follicular fluid correlate with embryo quality during in vitro fertilization cycles. Fertil Steril 87(2):279–282CrossRef
Zhao CJ, Li N, Deng XM (2003) The establishment of method for identifying SNP genotype by CRS-PCR. Yi Chuan 25(3):327–329PubMed
Zheng J, Ather JL, Sonstegard TS, Kerr DE (2005) Characterization of the infection-responsive bovine lactoferrin promoter. Gene 353(1):107–117PubMedCrossRef
Metadata
Title
Assessment of a single-nucleotide polymorphism in specific protein 1 (SP1)-binding site of the Lactoferrin promoter region as potential genetic markers for uterine infection in dairy cows
Authors
Fahimeh Pourzal
Hassan Sharifiyazdi
Abdolah Mirzaei
Publication date
01-09-2014
Publisher
Springer London
Published in
Comparative Clinical Pathology / Issue 5/2014
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI
https://doi.org/10.1007/s00580-013-1781-x

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