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BMC Complementary Medicine and Therapies

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Open Access 01-12-2014 | Research article

Assessing the antibiotic potential of essential oils against Haemophilus ducreyi

Authors: Zachary Lindeman, Molly Waggoner, Audra Batdorff, Tricia L Humphreys

Published in: BMC Complementary Medicine and Therapies | Issue 1/2014

Abstract

Background

Haemophilus ducreyi is the bacterium responsible for the genital ulcer disease chancroid, a cofactor for the transmission of HIV, and it is resistant to many antibiotics. With the goal of exploring possible alternative treatments, we tested essential oils (EOs) for their efficacy as antimicrobial agents against H. ducreyi.

Methods

We determine the minimum inhibitory concentration (MIC) of Cinnamomum verum (cinnamon), Eugenia caryophyllus (clove) and Thymus satureioides (thyme) oil against 9 strains of H. ducreyi using the agar dilution method. We also determined the minimum lethal concentration for each oil by subculturing from the MIC plates onto fresh agar without essential oil. For both tests, we used a 2-way ANOVA to evaluate whether antibiotic-resistant strains had a different sensitivity to the oils relative to non-resistant strains.

Results

All 3 oils demonstrated excellent activity against H. ducreyi, with MICs of 0.05 to 0.52 mg/mL and MLCs of 0.1-0.5 mg/mL. Antibiotic-resistant strains of H. ducreyi were equally susceptible to these 3 essential oils relative to non-resistant strains (p = 0.409).

Conclusion

E. caryophyllus, C. verum and T. satureioides oils are promising alternatives to antibiotic treatment for chancroid.

Available only for authorised users

Sexually transmitted diseases treatment guidelines, diseases characterized by genital, anal, or perianal ulcers. 2010,http://www.cdc.gov/std/treatment/2010/genital-ulcers.htm#chancroid,

Rutanarugsa A, Vorachit M, Polnikorn N, Jayanetra P: Drug resistance of Haemophilus ducreyi. Southeast Asian J Trop Med Public Health. 1990, 21: 185-193.PubMed

Brunton JL, Maclean I, Ronald AR, Albritton WL: Plasmid-mediated ampicillin resistance in Haemophilus ducreyi. Antimicrob Agents Chemother. 1979, 15: 294-299. 10.1128/AAC.15.2.294.CrossRefPubMedPubMedCentral

Morse SA: Chancroid and Haemophilus ducreyi. Clin Microbiol Rev. 1989, 2: 137-157.CrossRefPubMedPubMedCentral

Steen R: Eradicating chancroid. Bull World Health Organ. 2001, 79: 818-826.PubMedPubMedCentral

Rios JL, Recio MC: Medicinal plants and antimicrobial activity. J Ethnopharmacol. 2005, 100: 80-84. 10.1016/j.jep.2005.04.025.CrossRefPubMed

Warnke PH, Becker ST, Podschun R, Sivananthan S, Springer IN, Russo PA, Wiltfang J, Fickenscher H, Sherry E: The battle against multi-resistant strains: renaissance of antimicrobial essential oils as a promising force to fight hospital-acquired infections. J Craniomaxillofac Surg. 2009, 37: 392-397. 10.1016/j.jcms.2009.03.017.CrossRefPubMed

Van Vuuren SF, Naidoo D: An antimicrobial investigation of plants used traditionally in southern Africa to treat sexually transmitted infections. J Ethnopharmacol. 2010, 130 (3): 552-558. 10.1016/j.jep.2010.05.045.CrossRefPubMed

Mayaud L, Carricajo A, Zhiri A, Aubert G: Comparison of bacteriostatic and bactericidal activity of 13 essential oils against strains with varying sensitivity to antibiotics. Lett Appl Microbiol. 2008, 47: 167-173. 10.1111/j.1472-765X.2008.02406.x.CrossRefPubMed

10.

Haydock AK, Martin DH, Morse SA, Cammarata C, Mertz KJ, Totten PA: Molecular characterization of Haemophilus ducreyi strains from Jackson, Mississippi, and New Orleans, Louisiana. J Infect Dis. 1999, 179: 1423-1432. 10.1086/314771.CrossRefPubMed

11.

Hammer KA, Carson CF, Riley TV: In vitro susceptibilities of lactobacilli and organisms associated with bacterial vaginosis to Melaleuca alternifolia (tea tree) oil. Antimicrob Agents Chemother. 1999, 43: 196-PubMedPubMedCentral

12.

Linhares IM, Giraldo PC, Baracat EC: New findings about vaginal bacterial flora. Rev Assoc Med Bras. 2010, 56 (3): 370-374. 10.1590/S0104-42302010000300026.CrossRefPubMed

13.

Ravel J, Gajer P, Fu L, Mauck CK, Koenig SS, Sakamoto J, Motsinger-Reif AA, Doncel GF, Zeichner SL: Twice-daily application of HIV microbicides alter the vaginal microbiota. MBio. 2012, 3 (6): doi:10.1128/mBio.00370-12

14.

Lis-Balchin M: Possible health and safety problems in the use of novel plant essential oils and extracts in aromatherapy. J R Soc Promot Health. 1999, 119: 240-243. 10.1177/146642409911900407.CrossRefPubMed

15.

Reichling J, Schnitzler P, Suschke U, Saller R: Essential oils of aromatic plants with antibacterial, antifungal, antiviral, and cytotoxic properties–an overview. Forsch Komplementmed. 2009, 16: 79-90.CrossRefPubMed

The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/14/172/prepub

Title: Assessing the antibiotic potential of essential oils against Haemophilus ducreyi
Authors: Zachary Lindeman
Molly Waggoner
Audra Batdorff
Tricia L Humphreys
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2014
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/1472-6882-14-172

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Assessing the antibiotic potential of essential oils against Haemophilus ducreyi

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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