Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
BMC Musculoskeletal Disorders
Published in: BMC Musculoskeletal Disorders 1/2018

Open Access 01-12-2018 | Research article

Articular cartilage gene expression patterns in the tissue surrounding the impact site following applications of shear and axial loads

Authors: R. S. McCulloch, P. L. Mente, A. T. O’Nan, M. S. Ashwell

Published in: BMC Musculoskeletal Disorders | Issue 1/2018

Login to get access

Abstract

Background

Osteoarthritis is a degradative joint disease found in humans and commercial swine which can develop from a number of factors, including prior joint trauma. An impact injury model was developed to deliver in vitro loads to disease-free porcine patellae in a model of OA.

Methods

Axial impactions (2000 N normal) and shear impactions (500 N normal with induced shear forces) were delivered to 48 randomly assigned patellae. The patellae were then cultured for 0, 3, 7, or 14 days following the impact. Specimens in the tissue surrounding the loading site were harvested and expression of 18 OA related genes was studied via quantitative PCR. The selected genes were previously identified from published work and fell into four categories: cartilage matrix, degradative enzymes, inflammatory response, and apoptosis.

Results

Type II collagen (Col2a1) showed significantly lower expression in shear vs. axial adjacent tissue at day 0 and 7 (fold changes of 0.40 & 0.19, respectively). In addition, higher expression of degradative enzymes and Fas, an apoptosis gene, was observed in the shear specimens.

Conclusions

The results suggest that a more physiologically valid shear load may induce more damage to surrounding articular cartilage than a normal load alone.
Appendix
Available only for authorised users
Literature
1.
Arden N, Nevitt MC. Osteoarthritis: epidemiology. Best Pract Res Clin Rheumatol. 2006;20:3–25.CrossRef
2.
Rai MF, Duan X, Quirk JD, Holguin N, Schmidt EJ, Chinzei N, Silva MJ, Sandell LJ. Post-traumatic osteoarthritis in mice following mechanical injury to the synovial joint. Sci Rep. 2017;7:45223.CrossRef
3.
Goldring MB, Culley KL, Otero M. Pathogenesis of osteoarthritis in General. Cham, Switzerland: Springer; 2017.
4.
Buckwalter JA, Lane NE. Athletics and osteoarthritis. Am J Sports Med. 1997;25:873–81.CrossRef
5.
Ficklin T, Thomas G, Barthel JC, Asanbaeva A, Thonar EJ, Masuda K, Chen AC, Sah RL, Davol A, Klisch SM. Articular cartilage mechanical and biochemical property relations before and after in vitro growth. J Biomech. 2007;40:3607–14.CrossRef
6.
Wilson W, van Burken C, van Donkelaar C, Buma P, van Rietbergen B, Huiskes R. Causes of mechanically induced collagen damage in articular cartilage. J Orthop Res. 2006;24:220–8.CrossRef
7.
Ashwell MS, O'Nan AT, Gonda MG, Mente PL. Gene expression profiling of chondrocytes from a porcine impact injury model. Osteoarthr Cartil. 2008;16:936–46.CrossRef
8.
Aigner T, Dudhia J. Genomics of osteoarthritis. Curr Opin Rheumatol. 2003;15:634–40.CrossRef
9.
Salzmann GM, Nuernberger B, Schmitz P, Anton M, Stoddart MJ, Grad S, Milz S, Tischer T, Vogt S, Gansbacher B. Physicobiochemical synergism through gene therapy and functional tissue engineering for in vitro chondrogenesis. Tissue Eng Part A. 2009;15:2513–24.CrossRef
10.
Aigner T, Zien A, Hanisch D, Zimmer R. Gene expression in chondrocytes assessed with use of microarrays. J Bone Jt Surg. 2003;85:117–23.CrossRef
11.
Kapitanov GI, Ayati BP, Martin JA. Modeling the effect of blunt impact on mitochondrial function in cartilage: implications for development of osteoarthritis. PeerJ. 2017;5:e3468.CrossRef
12.
Maerz T, Newton MD, Kurdziel MD, Altman P, Anderson K, Matthew HW, Baker KC. Articular cartilage degeneration following anterior cruciate ligament injury: a comparison of surgical transection and noninvasive rupture as preclinical models of post-traumatic osteoarthritis. Osteoarthr Cartil. 2016;24(11):1918–27.CrossRef
13.
Wiegant K, Intema F, Van Roermund PM, Barten-van Rijbroek AD, Doornebal A, Hazewinkel HA, Lafeber FP, Mastbergen SC. Evidence of cartilage repair by joint distraction in a canine model of osteoarthritis. Arthritis Rheumatol. 2015;67(2):465–74.CrossRef
14.
Martin JA, Anderson DD, Goetz JE, Fredericks D, Pedersen DR, Ayati BP, Marsh JL, Buckwalter JA. Complementary models of post-traumatic osteoarthritis reveal cellular responses to contact stress that damage articular cartilage and present targets for intervention. J Orthop Res. 2017;35(3):515.CrossRef
15.
Zhao Y, Liu X, Mo D, Chen Q, Chen Y. Analysis of reasons for sow culling and seasonal effects on reproductive disorders in southern China. Anim Reprod Sci. 2015;159:191–7.CrossRef
16.
Crenshaw TD. Arthritis or OCD-identification and prevention. Adv Pork Prod. 2006;17:199.
17.
Kirk RK, Svensmark B, Ellegaard LP, Jensen HE. Locomotive disorders associated with sow mortality in Danish pig herds. Transbound Emerg Dis. 2005;52:423–8.
18.
Stavrakakis S, Guy JH, Syranidis I, Johnson GR, Edwards SA. Pre-clinical and clinical walking kinematics in female breeding pigs with lameness: a nested case-control cohort study. Vet J. 2015;205:38–43.CrossRef
19.
Muirhead MR, Alexander TJL. Chapter 9: Managing health in the weaner, grower and finishing periods. In: Carr J, editor. Managing Pig Heath: A Reference for the Farm. 2nd ed; 2013. p. 388.
20.
Lee KB, Hui JH, Song IC, Ardany L, Lee EH. Injectable mesenchymal stem cell therapy for large cartilage defects—a porcine model. Stem Cells. 2007;25(11):2964–71.CrossRef
21.
Gelse K, Mühle C, Franke O, Park J, Jehle M, Durst K, Göken M, Hennig F, Mark KV, Schneider H. Cell-based resurfacing of large cartilage defects: long-term evaluation of grafts from autologous transgene-activated periosteal cells in a porcine model of osteoarthritis. Arthritis & Rheumatism: official. J Am Coll Rheumatol. 2008;58(2):475–88.
22.
Goldring MB. The role of cytokines as inflammatory mediators in osteoarthritis: lessons from animal models. Connect Tissue Res. 1999;40(1):1–1.CrossRef
23.
Sieker JT, Proffen BL, Waller KA, Chin KE, Karamchedu NP, Akelman MR, Perrone GS, Kiapour AM, Konrad J, Murray MM, Fleming BC. Transcriptional profiling of articular cartilage in a porcine model of early post-traumatic osteoarthritis. J Orthop Res. 2018;36(1):318–29.PubMed
24.
McCulloch RS, Ashwell MS, Maltecca C, O'Nan AT, Mente PL. Progression of gene expression changes following a mechanical injury to articular cartilage as a model of early stage osteoarthritis. Arthritis. 2014:371426.
25.
McCulloch RS, Ashwell MS, T O’Nan A, Mente PL. Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage. J Anim Sci Biotechnol 2012;3:36.
26.
Steibel JP, Poletto R, Coussens PM, Rosa GJ. A powerful and flexible linear mixed model framework for the analysis of relative quantification RT-PCR data. Genomics. 2009;94:146–52.CrossRef
27.
Reiner A, Yekutieli D, Benjamini Y. Identifying differentially expressed genes using false discovery rate controlling procedures. Bioinformatics. 2003;19:368–75.CrossRef
28.
Xu Y, Barter MJ, Swan DC, Rankin KS, Rowan AD, Santibanez-Koref M, Loughlin J, Young DA. Identification of the pathogenic pathways in osteoarthritic hip cartilage: commonality and discord between hip and knee OA. Osteoarthr Cartil. 2012;20:1029–38.CrossRef
29.
Peffers MJ, Liu X, Clegg PD. Transcriptomic signatures in cartilage ageing. Arthritis Res Ther. 2013;15:R98.CrossRef
30.
Fitzgerald JB, Jin M, Dean D, Wood DJ, Zheng MH, Grodzinsky AJ. Mechanical compression of cartilage explants induces multiple time-dependent gene expression patterns and involves intracellular calcium and cyclic AMP. J Biol Chem. 2004;279:19502–11.CrossRef
31.
Van der Kraan PM, Van den Berg WB. Chondrocyte hypertrophy and osteoarthritis: role in initiation and progression of cartilage degeneration? Osteoarthr Cartil. 2012;20:223–32.CrossRef
32.
Swingler TE, Waters JG, Davidson RK, Pennington CJ, Puente XS, Darrah C, Cooper A, Donell ST, Guile GR, Wang W. Degradome expression profiling in human articular cartilage. Arthritis Res Ther. 2009;11:R96.CrossRef
33.
Kim HA, Lee YJ, Seong S, Choe KW, Song YW. Apoptotic chondrocyte death in human osteoarthritis. J Rheumatol. 2000;27:455–62.PubMed
Metadata
Title
Articular cartilage gene expression patterns in the tissue surrounding the impact site following applications of shear and axial loads
Authors
R. S. McCulloch
P. L. Mente
A. T. O’Nan
M. S. Ashwell
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Musculoskeletal Disorders / Issue 1/2018
Electronic ISSN: 1471-2474
DOI
https://doi.org/10.1186/s12891-018-2374-2

Other articles of this Issue 1/2018

BMC Musculoskeletal Disorders 1/2018 Go to the issue

Research article

How does the sagittal spinal balance of the scoliotic population deviate from the asymptomatic population?

Research article

Early detection of osteoarthritis in rabbits using MRI with a double-contrast agent

Research article

Reattachment of the flexor and extensor tendons at the epicondyle in elbow instability: a biomechanical comparison of techniques

Research article

Age and sex related differences in shoulder abduction fatigue

Research article

Acetabular cup orientation and postoperative leg length discrepancy in patients undergoing elective total hip arthroplasty via a direct anterior and anterolateral approaches

Research article

Predicting the outcome of conservative treatment with physiotherapy in adults with shoulder pain associated with partial-thickness rotator cuff tears – a prognostic model development study