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Open Access 01-06-2019 | Original Article

Arctigenin induces apoptosis in human hepatoblastoma cells

Authors: Atsuki Naoe, Tomonori Tsuchiya, Yasuhiro Kondo, Naoko Uga, Shunsuke Watanabe, Toshihiro Yasui, Fujio Hara, Tatsuya Suzuki

Published in: Pediatric Surgery International | Issue 6/2019

Abstract

Purpose

Arctigenin has been shown to have anti-tumor effects in various types of cancers. This study was conducted to verify these effects in the human-derived hepatoblastoma cell line, HUH-6 clone 5 (hereinafter, HUH-6).

Methods

Arctigenin was added to cultured HUH-6 cells, and cellular activity was evaluated by MTS assay. To determine the relationship between reduced cellular activity and apoptosis, we measured the activities of caspase 3/7, 8, and 9 and conducted flow cytometry with Annexin V/PI staining.

Results

The MTS assay revealed that cellular activity decreased after arctigenin treatment in a concentration-dependent manner (IC₅₀ = 4 µM). To investigate apoptosis induction, activity assays of caspase 3/7, 8, and 9 were performed. While caspase 3/7 and 8 exhibited high activity, caspase 9 showed no activity. Thus, apoptosis induction may have involved the action of tumor necrosis factor receptor 1 (TNFR1). Flow cytometry conducted with Annexin V/PI staining revealed the occurrence of early apoptosis.

Conclusion

We found that arctigenin has anti-tumor effects in HUH-6 cells in a concentration-dependent manner. Arctigenin may have exerted its anti-tumor effect by inducing apoptosis via TNFR1, which recruits Complex IIa to activate caspase 8 and 3/7. These results may be useful for developing therapeutic agents for hepatoblastoma.

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Title: Arctigenin induces apoptosis in human hepatoblastoma cells
Authors: Atsuki Naoe
Tomonori Tsuchiya
Yasuhiro Kondo
Naoko Uga
Shunsuke Watanabe
Toshihiro Yasui
Fujio Hara
Tatsuya Suzuki
Publication date: 01-06-2019
Publisher: Springer Berlin Heidelberg
Published in: Pediatric Surgery International / Issue 6/2019
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-019-04473-6

At a glance: The STEP trials

Springer Medicine

Arctigenin induces apoptosis in human hepatoblastoma cells

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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