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01-10-2004 | Original Article

Apoptosis of gastric cancer cell line MKN45 by photodynamic treatment with Photofrin

Authors: Kenichiro Takahira, Munetaka Sano, Hajime Arai, Hiroyuki Hanai

Published in: Lasers in Medical Science | Issue 2/2004

Abstract

The aim of this study was to investigate the mechanism of cell death by photodynamic therapy (PDT) in the gastric cancer cell line MKN45 with focus on the mechanism of apoptosis. Gastric cancer cells (MKN45) were incubated with Photofrin for up to 24 h before exposure to He-Cd laser (441 nm, 1 J/cm²). Cell viability was assessed by the methyl-tetra-zolium assay after exposure to light. A 95% cell death (LD₉₅) was measured with 10 μg/ml of Photofrin. DNA ladder formation and chromatin condensation were seen within 60 min. Caspase-3–like and caspase-9–like activities increased from 15 min after exposure to light. Reduction of rhodamine 123 uptake started at 30 min. Caspase-inhibitor VAD-fmk (10 mM) inhibited apoptosis, but did not influence cell viability. In conclusion, Photofrin-mediated PDT in the gastric cancer cell line MKN45 induces apoptosis within 60 min, and mitochondrial damage is likely as the first event of apoptosis.

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Title: Apoptosis of gastric cancer cell line MKN45 by photodynamic treatment with Photofrin
Authors: Kenichiro Takahira
Munetaka Sano
Hajime Arai
Hiroyuki Hanai
Publication date: 01-10-2004
Publisher: Springer-Verlag
Published in: Lasers in Medical Science / Issue 2/2004
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-004-0297-7

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Apoptosis of gastric cancer cell line MKN45 by photodynamic treatment with Photofrin

Abstract

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Abstract

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