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European Journal of Plastic Surgery
Published in: European Journal of Plastic Surgery 6/2017

01-12-2017 | Experimental Study

Apocynin and dimethyl sulfoxide synergistically protect against ischemia-reperfusion injury in a rat hind limb ischemia-reperfusion model

Authors: Alpay Duran, Sinem Ciloglu, Ozer Sehirli, Ahmet Yigit, Hasan Buyukdogan, Aslı Duran, Kubra Elcioğlu, Levent Kabasakal

Published in: European Journal of Plastic Surgery | Issue 6/2017

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Abstract

Background

Acute ischemia and reperfusion in extremities ignite a complicated inflammatory cycle and lead to damage in the extremities and target tissues.

Methods

Rats were randomly divided into four groups. Each experimental group contained eight rats. Group 1 was the sham surgery group. Group 2 was the control I/R group with intraperitoneal serum physiologic (SP) treatment. Group 3 was the I/R group with vehicle treatment (8% DMSO). Group 4 was the I/R group with 20 mg/kg apocynin (vehicle 8% DMSO). The potential protective effects of apocynin and vehicle (DMSO) in the rat hind limb ischemia-reperfusion model were evaluated by measuring the levels of plasma pro-inflammatory cytokines and oxidative stress markers in muscle tissue.

Results

The results revealed that both apocynin and vehicle (DMSO) alone increased the antioxidative capacity of ischemic tissue and reduced the levels of plasma proinflammatory cytokines compared to control I/R group. There was a statically more significant effect in the apocynin (vehicle 8% DMSO)-treated group 4.

Conclusions

We observed that apocynin and DMSO synergistically prevent the I/R injury in rat hind limb ischemia-reperfusion injury model and meaningfully decrease the inflammatory parameters.
Level of Evidence: Not ratable.
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Metadata
Title
Apocynin and dimethyl sulfoxide synergistically protect against ischemia-reperfusion injury in a rat hind limb ischemia-reperfusion model
Authors
Alpay Duran
Sinem Ciloglu
Ozer Sehirli
Ahmet Yigit
Hasan Buyukdogan
Aslı Duran
Kubra Elcioğlu
Levent Kabasakal
Publication date
01-12-2017
Publisher
Springer Berlin Heidelberg
Published in
European Journal of Plastic Surgery / Issue 6/2017
Print ISSN: 0930-343X
Electronic ISSN: 1435-0130
DOI
https://doi.org/10.1007/s00238-017-1309-8

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