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01-07-2013 | Original Article

Apo E polymorphism among Egyptian patients with essential hypertension

Authors: Dina ELAbd, Mona Lotfi Essawi, Hala Soliman, Abeer Mohy, Iman Atef Mandour, Nouran Mohammed Sedky, Amr ELFaramawy

Published in: Comparative Clinical Pathology | Issue 4/2013

Abstract

Hypertension (HTN) is a chronic condition of concern due to its role in the causation of coronary artery disease (CAD), stroke, and other vascular complications. Essential hypertension (EH) is a multifactorial disorder arising from the influence of several susceptibility genes and environmental stimuli. Apolipoprotein E (Apo E) plays an essential role in clearance of chylomicron remnants and very low-density lipoproteins (VLDL). Apo E gene has three alleles (E2, E3, and E4) that give rise to six different genotypes. A significant association of E4 allele has been observed with HTN in addition to the other well-known risk factors and positive family history. Carriers of E4 allele form a higher risk group showing greater susceptibility to CAD. These observations emphasize the need of genotyping Apo E in patients with EH as an important molecular tool in personalized medicine. The aim of this work was to study the association between Apo E gene polymorphism and EH in Egyptian patients as well as correlating different Apo E genotypes with serum lipids. The study was conducted on 50 patients with EH and 50 age-matched controls. DNA analysis was performed using polymerase chain reaction restriction fragment length polymorphism. The E3/E3 genotype was found in 85.42 % of patients, compared to 80 % in controls. E3/E4 (8.33 %) and E2/E3 (6.25 %) were lower in patients compared to controls 12 and 8 %, respectively. E4/E4 and E2/E2 genotypes were only found in two patients (4 %). Total cholesterol and low-density lipoprotein were significantly higher in E3/E4 as compared to E3/E3 and E2/E3. However, there was no significant difference in triglyceride, high-density lipoprotein, and VLDL.

Al-Khedhairy A (2004) Apolipoprotein E polymorphism in Saudis. Mol Biol Rep 31(4):257–260PubMedCrossRef

Al-Yahyaee SA, Al-Kindi MN, Al-Bahrani AH (2005) Distribution of apolipoprotein E alleles in the Omani population. Med Princ Pract 14(2):73–78PubMedCrossRef

Bhavani AB, Sastry KB, Reddy NK, Padma T (2005) Lipid profile and apolipoprotein E polymorphism in essential hypertension. Indian Heart J 57(2):151–157PubMed

Chobanian AV, Bakris GL, Black HR, Cushman WC, Green LA, Izzo JL Jr, Jones DW, Materson BJ, Oparil S, Wright JT Jr, Roccella EJ, Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure, National Heart, Lung, and Blood Institute, National High Blood Pressure Education Program Coordinating Committee (2003) Seventh report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure. Hypertension 42(6):1206–1252PubMedCrossRef

Couderc R, Mahieux F, Bailleul S, Fenelon G, Mary R, Fermanian J (1993) Prevalence of apolipoprotein E phenotypes in ischemic cerebrovascular disease. Stroke 24(5):661–664PubMedCrossRef

Davignon J, Gregg RE, Sing CF (1988) Apolipoprotein E polymorphism and atherosclerosis. Arteriosclerosis 8(1):1–21PubMedCrossRef

Dembińska-Kieć A, Kawecka-Jaszcz K, Kwaśniak M, Guevara I, Pankiewicz J, Malczewska-Malec M, Iwanejko J, Hartwich J, Zdzienicka A, Stochmal A, Leszczyńska-Gołabek I (1998) Apo E isoforms, insulin output and plasma lipid levels in essential hypertension. Eur J Clin Invest 28(2):95–99PubMedCrossRef

Friedewald WT, Levy RI, Fredrickson DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of preparative ultracentrifuge. Clin Chem 18(6):499–502PubMed

Ibrahim MM (2001) Report on the consultation on establishing an integrated regional noncommunicable disease network. World Health Organization (WHO), Geneva

Imazu M, Yamamoto H, Toyofuku M, Watanabe T, Okubo M, Egusa G, Yamakido M, Kohno N (2001) Association of apolipoprotein E phenotype with hypertension in Japanese-Americans: data from the Hawaii–Los Angeles–Hiroshima study. Hypertens Res 24(5):523–529PubMedCrossRef

Jemaa R, Elasmi M, Naouali C, Feki M, Kallel A, Souissi M, Sanhaji H, Hadj Taïeb S, Souheil O, Kaabachi N (2006) Apolipoprotein E polymorphism in the Tunisian population: frequency and effect on lipid parameters. Clin Biochem 39(8):816–820PubMedCrossRef

Katsuya T, Baba S, Ishikawa K, Mannami T, Fu Y, Inamoto N, Asai T, Fukuda M, Higaki J, Ogata J, Ogihara T (2002) Epsilon 4 allele of apolipoprotein E gene associates with lower blood pressure in young Japanese subjects: the Suita study. J Hypertens 20(10):2017–2021PubMedCrossRef

Kearney PM, Whelton M, Reynolds K, Muntner P, Whelton PK, He J (2005) Global burden of hypertension: analysis of worldwide data. Lancet 365(9455):217–223PubMed

Kesäniemi YA, Ehnholm C, Miettinen TA (1987) Intestinal cholesterol absorption efficiency in man is related to apoprotein E phenotype. J Clin Invest 80(2):578–581PubMedCrossRef

Li X, Du Y, Du Y, Huang X (2003) Association of apolipoprotein E gene polymorphism with essential hypertension and its complications. Clin Exp Med 2(4):175–179PubMedCrossRef

Miller SA, Dykes DD, Polesky HF (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16(3):1215–1225PubMedCrossRef

Niu W, Guo X, Su Y, Qiu C (2007) Apolipoprotein E and low-density lipoprotein receptor gene polymorphisms in dyslipidemias-associated essential hypertension. J Hum Hypertens 21(4):337–339PubMed

Pierdomenico SD, Di Nicola M, Esposito AL, Di Mascio R, Ballone E, Lapenna D, Cuccurullo F (2009) Prognostic value of different indices of blood pressure variability in hypertensive patients. Am J Hypertens 22(8):842–847PubMedCrossRef

Richard P, Thomas G, de Zulueta MP, de Gennes JL, Thoma M, Cassaigne A, Béréziat G, Iron A (1994) Common and rare genotypes of human apolipoprotein E determined by specific restriction profiles of polymerase chain reaction-amplified DNA. Clin Chem 40(1):24–29PubMed

Singh PP, Singh M, Mastana SS (2002) Genetic variation of apolipoproteins in North Indians. Hum Biol 74(5):673–682PubMedCrossRef

Wilson PW, Myers RH, Larson MG, Ordovas JM, Wolf PA, Schaefer EJ (1994) Apolipoprotein E alleles, dyslipidemia, and coronary heart disease. The Framingham Offspring Study. JAMA 272(21):1666–1671PubMedCrossRef

Yilmaz H, Isbir T, Ağaçhan B, Aydin M (2001) Is epsilon4 allele of apolipoprotein E associated with more severe end-organ damage in essential hypertension? Cell Biochem Funct 19(3):191–195PubMedCrossRef

Title: Apo E polymorphism among Egyptian patients with essential hypertension
Authors: Dina ELAbd
Mona Lotfi Essawi
Hala Soliman
Abeer Mohy
Iman Atef Mandour
Nouran Mohammed Sedky
Amr ELFaramawy
Publication date: 01-07-2013
Publisher: Springer London
Published in: Comparative Clinical Pathology / Issue 4/2013
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-012-1476-8

At a glance: The STEP trials

Springer Medicine

Apo E polymorphism among Egyptian patients with essential hypertension

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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