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Clinical Oral Investigations
Published in: Clinical Oral Investigations 9/2014

01-12-2014 | Original Article

Apical periodontitis and periodontal disease increase serum IL-17 levels in normoglycemic and diabetic rats

Authors: Luciano Tavares Angelo Cintra, Renata Oliveira Samuel, Mariane Maffei Azuma, Clícia Pereira Ribeiro, Luis Gustavo Narciso, Valéria Marçal Felix de Lima, Dóris Hissako Sumida, Gilberto Aparecido Coclete, Eloi Dezan-Júnior, João Eduardo Gomes-Filho

Published in: Clinical Oral Investigations | Issue 9/2014

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Abstract

Objectives

This study aimed to evaluate the influence of apical periodontitis (AP) and/or periodontal disease (PD) on serum interleukin-17 (IL-17) levels in a rat model of diabetes mellitus (DM).

Methods

Eighty male Wistar rats were divided into eight groups of ten animals each: normoglycemic, AP, PD, AP+PD, DM, DM+AP, DM+PD, and DM+AP+PD. DM was induced using streptozotocin, AP by dental pulp exposure to the oral environment, and PD by periodontal ligature. The animals were sacrificed after 30 days, and venous blood samples were collected via cardiac puncture to determine the serum IL-17 and neutrophil levels. The maxillae were dissected and processed for radiographic analysis. The periapical lesion areas were quantified in pixels. The total assessed values were tabulated according to each experimental group and were statistically analyzed using Spearman’s correlation and Kruskal-Wallis test (p < 0.05).

Results

A significant difference in the serum IL-17 levels was observed between the groups without oral infections and the groups with AP+PD-associated lesions, regardless of the presence of DM (p < 0.05). Diabetes increased the neutrophil levels, regardless of the presence of oral infection. However, a combination of two oral infections increased the neutrophil levels in DM rats (p < 0.05). The level of bone resorption lesions was greater in DM rats than in normoglycemic rats (p < 0.05).

Conclusion

The combination of AP and PD increased the serum IL-17 levels in DM and normoglycemic rats and increased the neutrophil levels in DM rats. Diabetes increased the neutrophil levels and bone resorption in rats.

Clinical significance

AP is capable of potentiating systemic inflammatory changes when associated with PD, and increases in blood glucose can accelerate the pathogenesis of oral infections.
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Metadata
Title
Apical periodontitis and periodontal disease increase serum IL-17 levels in normoglycemic and diabetic rats
Authors
Luciano Tavares Angelo Cintra
Renata Oliveira Samuel
Mariane Maffei Azuma
Clícia Pereira Ribeiro
Luis Gustavo Narciso
Valéria Marçal Felix de Lima
Dóris Hissako Sumida
Gilberto Aparecido Coclete
Eloi Dezan-Júnior
João Eduardo Gomes-Filho
Publication date
01-12-2014
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 9/2014
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-014-1192-7

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