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01-05-2020 | Aortic Valve Stenosis | Correction
Correction to: Warfarin calcifies human aortic valve interstitial cells at high-phosphate conditions via pregnane X receptor
Published in: Journal of Bone and Mineral Metabolism | Issue 3/2020
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In the original publication of the article, part of Fig. 1 was published incorrectly. The correct version of Fig. 1 is provided below.
Fig. 1
Establishment of warfarin-induced calcifcation conditions of HAVICs using a range of phosphate concentrations. HAVICs were cultured in α-MEM containing 10% fetal bovine serum (FBS). After reaching 90% confuency (Day 0), HAVICs were further cultured in medium containing a range of inorganic phosphate (Pi) concentrations (1.0–3.8 mM) for 7 days. a Representative images of Alizarin Red S staining (upper images) of HAVICs from both the AS and non-AS groups and quantifcation of Alizarin Red S staining on day 7 following cetyl-pyridinium chloride extraction. The amount of released dye was quantifed by spectrophotometry at 550 nm. All ratios were calculated versus the Pi (1.0 mM) group on day 7. Bars represent the mean ± SEM (n = 4). A signifcant diference (P < 0.001) when compared with control cells (Pi = 1.0 mM) or Pi = 3.2 mM on day 7 is denoted by ‘Hash’. The mean threshold concentration of Pi for calcifcation was 3.2 mM (Blue bar). b Representative images of Alizarin Red S staining of HUVECs following tumor necrosis factor-α (TNF-α, 30 ng/ml)-induced calcifcation in high-phosphate (Pi) medium (3.2 mM). Sodium phosphonoformate (PFA, 0.1 mM) was used as a selective inhibitor for PiT-1, a phosphate transporter. c Typical images of Alizarin Red S staining of HAVICs prepared from aortic valves afected by calcifc aortic valve stenosis (AS) and HAVICs prepared from aortal valves afected by aortic regurgitation but without any signs of calcifcation (non-AS) in the presence or absence of 1 µM warfarin in high-Pi medium (color fgure online)
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