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BMC Complementary Medicine and Therapies

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Open Access 01-12-2011 | Research article

Antioxidative and in vitro antiproliferative activity of Arctium lappa root extracts

Authors: Fabricia S Predes, Ana LTG Ruiz, João E Carvalho, Mary A Foglio, Heidi Dolder

Published in: BMC Complementary Medicine and Therapies | Issue 1/2011

Abstract

Background

Arctium lappa, known as burdock, is widely used in popular medicine for hypertension, gout, hepatitis and other inflammatory disorders. Pharmacological studies indicated that burdock roots have hepatoprotective, anti-inflammatory, free radical scavenging and antiproliferative activities. The aim of this study was to evaluate total phenolic content, radical scavenging activity by DPPH and in vitro antiproliferative activity of different A. lappa root extracts.

Methods

Hot and room temperature dichloromethanic, ethanolic and aqueous extracts; hydroethanolic and total aqueous extract of A. lappa roots were investigated regarding radical scavenging activity by DPPH, total phenolic content by Folin-Ciocalteau method and antiproliferative in vitro activity was evaluated in human cancer cell lines. The hydroethanolic extract analyzed by high-resolution electrospray ionization mass spectroscopy.

Results

Higher radical scavenging activity was found for the hydroethanolic extract. The higher phenolic contents were found for the dichloromethane, obtained both by Soxhlet and maceration extraction and hydroethanolic extracts. The HRESI-MS demonstrated the presence of arctigenin, quercetin, chlorogenic acid and caffeic acid compounds, which were identified by comparison with previous data. The dichloromethane extracts were the only extracts that exhibited activity against cancer cell lines, especially for K562, MCF-7 and 786-0 cell lines.

Conclusions

The hydroethanolic extracts exhibited the strongest free radical scavenging activity, while the highest phenolic content was observed in Soxhlet extraction. Moreover, the dichloromethanic extracts showed selective antiproliferative activity against K562, MCF-7 and 786-0 human cancer cell lines.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/11/25/prepub

Title: Antioxidative and in vitro antiproliferative activity of Arctium lappa root extracts
Authors: Fabricia S Predes
Ana LTG Ruiz
João E Carvalho
Mary A Foglio
Heidi Dolder
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2011
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/1472-6882-11-25

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Antioxidative and in vitro antiproliferative activity of Arctium lappa root extracts

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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