Top

Published in:

01-10-2016 | Original Paper

Antioxidative activities of 62 wild mushrooms from Nepal and the phenolic profile of some selected species

Authors: Sonam Tamrakar, Hai Bang Tran, Marina Nishida, Satoru Kaifuchi, Hiroto Suhara, Katsumi Doi, Katsuya Fukami, Gopal Prasad Parajuli, Kuniyoshi Shimizu

Published in: Journal of Natural Medicines | Issue 4/2016

Abstract

Mushrooms have garnered immense popularity for their nutritional as well as medicinal values. The therapeutic potential of mushrooms in Nepal, a country well known for its biodiversity and natural medicinal resources, remains largely unstudied. Therefore, this study attempts to unveil the antioxidative properties of Nepalese wild mushrooms. Sixty-two wild mushroom samples were collected from several forests in different parts of Nepal. Ethanol and water extracts of the dried samples were tested for their antioxidative activities using total phenolic content (TPC), oxygen radical absorbance capacity (ORAC) assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and reducing power (RP) assays. Ethanol extracts of samples belonging to the order Hymenochaetales showed significantly high activity in all the assays. Inonotus clemensiae had an exceptionally high TPC of 643.2 mg gallic acid equivalent (GAE)/g extract and also exhibited the lowest EC₅₀ values in DPPH (0.081 mg/mL), ABTS (0.409 mg/mL), and EC_0.5 value in reducing power (RP; 0.031 mg/mL) assays. High-performance liquid chromatography (HPLC) analysis of the top ten samples with the highest TPC was done to identify the phenolic compounds in the extracts, followed by liquid chromatography–mass spectrometry (LC–MS) analysis for some unknown compounds. These findings highlight the very strong antioxidative activity of Nepalese mushrooms, and paves the way for further research to explore their economic potential.

Available only for authorised users

Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44–84. doi:10.1016/j.biocel.2006.07.001 CrossRefPubMed

Carocho M, Ferreira ICFR (2013) A review on antioxidants, prooxidants and related controversy: natural and synthetic compounds, screening and analysis methodologies and future perspectives. Food Chem Toxicol 51:15–25. doi:10.1016/j.fct.2012.09.021 CrossRefPubMed

Powers SK, Nelson WB, Hudson MB (2011) Exercise-induced oxidative stress in humans: cause and consequences. Free Radic Biol Med 51:942–950. doi:10.1016/j.freeradbiomed.2010.12.009 CrossRefPubMed

Blokhina O, Virolainen E, Fagerstedt KV (2003) Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot 91:179–194. doi:10.1093/aob/mcf118 CrossRefPubMedPubMedCentral

Moon JK, Shibamoto T (2009) Antioxidant assays for plant and food components. J Agric Food Chem 57:1655–1666. doi:10.1021/jf803537k CrossRefPubMed

Lobo V, Patil A, Phatak A, Chandra N (2010) Free radicals, antioxidants and functional foods: impact on human health. Pharmacogn Rev 4:118–126. doi:10.4103/0973-7847.70902 CrossRefPubMedPubMedCentral

Parke DV, Lewis DF (1992) Safety aspects of food preservatives. Food Addit Contam 9:561–577CrossRefPubMed

Barros L, Cruz T, Baptista P, Estevinho LM, Ferreira IC (2008) Wild and commercial mushrooms as source of nutrients and nutraceuticals. Food Chem Toxicol 46:2742–2747. doi:10.1016/j.fct.2008.04.030 CrossRefPubMed

Kalač P (2013) A review of chemical composition and nutritional value of wild-growing and cultivated mushrooms. J Sci Food Agric 93:209–218. doi:10.1002/jsfa.5960 CrossRefPubMed

10.

Wasser SP (2002) Review of medicinal mushrooms advances: good news from old allies. Herbal Gram 56:28–33

11.

Ajith A, Janardhanan K (2007) Indian medicinal mushrooms as a source of antioxidant and antitumor agents. J Clin Biochem Nutr 40:157–162. doi:10.3164/jcbn.40.157 CrossRef

12.

Lindequist U, Niedermeyer THJ, Jülich WD (2005) The pharmacological potential of mushrooms. Evid Based Complement Alternat Med 2:285–299. doi:10.1093/ecam/neh107 CrossRefPubMedPubMedCentral

13.

Vetaas OR, Grytnes JA (2002) Distribution of vascular plant species richness and endemic richness along the himalayan elevation gradient in Nepal. Glob Ecol Biogeogr 11:291–301. doi:10.1046/j.1466-822X.2002.00297.x CrossRef

14.

Adhikari MK (2012) Researches on the Nepalese mycoflora-2: checklist of macrofungi (mushrooms). Published by KS Adhikari, Kathmandu, Nepal. 20:1–84

15.

Christensen M, Bhattarai S, Devkota S, Larsen HO (2008) Collection and use of wild edible fungi in Nepal. Econ Bot 62:12–23. doi:10.1007/s12231-007-9000-9 CrossRef

16.

Hosaka K, Castellano MA (2008) Molecular phylogenetics of Geastrales with special emphasis on the position of Sclerogaster. Bull Natl Mus Nat Sci Ser B 34(4):161–173

17.

White TJ, Bruns T, Lee SJ, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315–322

18.

Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes—application to the identification of mycorrhizae and rusts. Mol Ecol 2:113–118. doi:10.1111/J.1365-294x.1993.Tb00005.X CrossRefPubMed

19.

Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16:144–158 (citeulike-article-id:7170825)

20.

Ou B, Huang D, Hampsch-Woodill M, Flanagan JA, Deemer EK (2002) Analysis of antioxidant activities of common vegetables employing oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays: a comparative study. J Agric Food Chem 50:3122–3128. doi:10.1021/jf0116606 CrossRefPubMed

21.

Miliauskas G, Venskutonis PR, van Beek TA (2004) Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem 85:231–237. doi:10.1016/j.foodchem.2003.05.007 CrossRef

22.

Zhu Q, Nakagawa T, Kishikawa A, Ohnuki K, Shimizu K (2015) In vitro bioactivities and phytochemical profile of various parts of the strawberry (Fragaria × ananassa var. Amaou). J Funct Foods 13:38–49. doi:10.1016/j.jff.2014.12.026 CrossRef

23.

Oyaizu M (1986) Studies on products of browning reaction—antioxidative activities of products of browning reaction prepared from glucosamine. Japanese J Nutr Diet 44:307–315. doi:10.5264/eiyogakuzashi.44.307 CrossRef

24.

Barros L, Ferreira MJ, Queirós B, Ferreira IC, Baptista P (2007) Total phenols, ascorbic acid, β-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chem 103:413–419. doi:10.1016/j.foodchem.2006.07.038 CrossRef

25.

Kim MY, Seguin P, Ahn JK et al (2008) Phenolic compound concentration and antioxidant activities of edible and medicinal mushrooms from Korea. J Agric Food Chem 56:7265–7270. doi:10.1021/jf8008553 CrossRefPubMed

26.

Asuquo JE, Etim EE (2011) Phytochemical and antinutrients evaluation of Oxyporus populinus. J Emerg Trends Eng Appl Sci 2:817–820

27.

Glamočlija J, Ćirić A, Nikolić M et al (2015) Chemical characterization and biological activity of chaga (Inonotus obliquus), a medicinal “mushroom”. J Ethnopharmacol 162:323–332. doi:10.1016/j.jep.2014.12.069 CrossRefPubMed

28.

Ferreira ICFR, Barros L, Abreu RMV (2009) Antioxidants in wild mushrooms. Curr Med Chem 16:1543–1560. doi:10.2174/092986709787909587 CrossRefPubMed

29.

Wong JY, Chye FY (2009) Antioxidant properties of selected tropical wild edible mushrooms. J Food Compos Anal 22:269–277. doi:10.1016/j.jfca.2008.11.021 CrossRef

30.

Hai Bang T, Suhara H, Doi K et al (2014) Wild mushrooms in Nepal: some potential candidates as antioxidant and ACE-inhibition sources. Evid Based Complement Alternat Med 2014:195305. doi:10.1155/2014/195305 CrossRefPubMedPubMedCentral

31.

Ou B, Hampsch-Woodill M, Prior RL (2001) Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. J Agric Food Chem 49:4619–4626. doi:10.1021/jf010586o CrossRefPubMed

32.

Teow CC, Den Truong V, McFeeters RF, Thompson RL, Pecota KV, Yencho GC (2007) Antioxidant activities, phenolic and β-carotene contents of sweet potato genotypes with varying flesh colours. Food Chem 103:829–838. doi:10.1016/j.foodchem.2006.09.033 CrossRef

33.

Arnao MB (2000) Some methodological problems in the determination of antioxidant activity using chromogen radicals: a practical case. Trends Food Sci Technol 11:419–421. doi:10.1016/S0924-2244(01)00027-9 CrossRef

34.

Apak R, Gorinstein S, Böhm V et al (2013) Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC Technical Report). Pure Appl Chem 85:957–998. doi:10.1351/PAC-REP-12-07-15 CrossRef

35.

Ferreira ICFR, Baptista P, Vilas-Boas M, Barros L (2007) Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: individual cap and stipe activity. Food Chem 100:1511–1516. doi:10.1016/j.foodchem.2005.11.043 CrossRef

36.

Novaes MRCG, Novaes LCG, Taveira VC (2007) Pharmacological effects of Agaricales fungi: a review of evidence. Rev Ciênc Méd Campinas 16:87–95

37.

Jacobo-Velázquez DA, Cisneros-Zevallos L (2009) Correlations of antioxidant activity against phenolic content revisited: a new approach in data analysis for food and medicinal plants. J Food Sci 74:R107–R113. doi:10.1111/j.1750-3841.2009.01352.x CrossRefPubMed

38.

Barros L, Baptista P, Estevinho LM, Ferreira ICFR (2007) Effect of fruiting body maturity stage on chemical composition and antimicrobial activity of Lactarius sp. mushrooms. J Agric Food Chem 55:8766–8771. doi:10.1021/jf071435+ CrossRefPubMed

39.

Sudheer S, Yeoh WK, Manickam S, Ali A (2016) Effect of ozone gas as an elicitor to enhance the bioactive compounds in Ganoderma lucidum. Postharvest Biol Technol 117:81–88. doi:10.1016/j.postharvbio.2016.01.014 CrossRef

40.

Lee I-K, Yun B-S (2011) Styrylpyrone-class compounds from medicinal fungi Phellinus and Inonotus spp., and their medicinal importance. J Antibiot (Tokyo) 64:349–359. doi:10.1038/ja.2011.2 CrossRef

41.

National Center for Biotechnology Information. Pub Chem Compound Database. Hispidin, CID = 54685921. Available online at: https://pubchem.ncbi.nlm.nih.gov/compound/54685921

Title: Antioxidative activities of 62 wild mushrooms from Nepal and the phenolic profile of some selected species
Authors: Sonam Tamrakar
Hai Bang Tran
Marina Nishida
Satoru Kaifuchi
Hiroto Suhara
Katsumi Doi
Katsuya Fukami
Gopal Prasad Parajuli
Kuniyoshi Shimizu
Publication date: 01-10-2016
Publisher: Springer Japan
Published in: Journal of Natural Medicines / Issue 4/2016
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-016-1013-1

At a glance: The STEP trials

Springer Medicine

Antioxidative activities of 62 wild mushrooms from Nepal and the phenolic profile of some selected species

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 4/2016

Chemical constituents from Inonotus obliquus and their antitumor activities

Evaluation and quantitative analysis of different growth periods of herb–arbor intercropping systems using HPLC and UV–vis methods coupled with chemometrics

Medicinal significance of naturally occurring cyclotetrapeptides

Application of a new method, orthogonal projection to latent structure (OPLS) combined with principal component analysis (PCA), to screening of prostaglandin E2 production inhibitory flavonoids in Scutellaria Root

Gastrodin reversed the traumatic stress-induced depressed-like symptoms in rats

Tetrahydroxanthones from Mongolian medicinal plant Gentianella amarella ssp. acuta