Published in:
Open Access
01-12-2013 | Research article
Antioxidant, anti-adipocyte differentiation, antitumor activity and anthelminticactivities against Anisakis simplex and Hymenolepis nana ofyakuchinone A from Alpinia oxyphylla
Authors:
Rong-Jyh Lin, Chuan-Min Yen, Tzung-Han Chou, Feng-Yu Chiang, Guey-Horng Wang, Ya-Ping Tseng, Lin Wang, Ting-Wei Huang, Hui-Chuan Wang, Leong-Perng Chan, Hsiou-Yu Ding, Chia-Hua Liang
Published in:
BMC Complementary Medicine and Therapies
|
Issue 1/2013
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Abstract
Background
Alpinia oxyphylla is a common remedy in traditional Chinese medicine.Yakuchinone A is a major constituent of A. oxyphylla andexhibits anti-inflammatory, antitumor, antibacterial, and gastric protectiveactivities.
Methods
Antioxidant and antitumor characteristics of yakuchinone A in skin cancer cells aswell as novel mechanisms for the inhibition of adipocyte differentiation,cestocidal activities against Hymenolepis nana adults, and nematocidalactivities against Anisakis simplex larvae are investigated.
Results
Yakuchinone A presents the ability of the removal of DPPH·andABTS+ free radicals and inhibition of lipid peroxidation.Yakuchinone A suppresses intracellular lipid accumulation during adipocytedifferentiation in 3 T3-L1 cells and the expressions of leptin andperoxisome proliferator-activated receptor γ(PPAR γ). Yakuchinone A induces apoptosis and inhibits cellproliferation in skin cancer cells. The inhibition of cell growth by yakuchinone Ais more significant for non-melanoma skin cancer (NMSC) cells than for melanoma(A375 and B16) and noncancerous (HaCaT and BNLCL2) cells. Treatment BCC cells withyakuchinone A shows down-regulation of Bcl-2, up-regulation of Bax, and anincrease in cleavage poly (ADP-ribose) polymerase (PARP). This suggests thatyakuchinone A induces BCC cells apoptosis through the Bcl-2-mediated signalingpathway. The anthelmintic activities of yakuchinone A for A. simplex arebetter than for H. nana.
Conclusions
In this work, yakuchinone A exhibits antioxidative properties, anti-adipocytedifferentiation, antitumor activity, and anthelmintic activities against A.simplex and H. nana.