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BMC Complementary Medicine and Therapies

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Open Access 01-12-2015 | Research article

Antioxidant activity and protection against oxidative-induced damage of Acacia shaffneri and Acacia farnesiana pods extracts: in vitro and in vivo assays

Authors: Claudia Delgadillo Puga, Mario Cuchillo Hilario, José Guillermo Espinosa Mendoza, Omar Medina Campos, Eduardo Molina Jijón, Margarita Díaz Martínez, Marsela Alejandra Álvarez Izazaga, José Ángel Ledesma Solano, José Pedraza Chaverri

Published in: BMC Complementary Medicine and Therapies | Issue 1/2015

Abstract

Background

Obesity is a worldwide public health issue, reaching epidemic condition in developing countries associated to chronic diseases. Oxidative damage is another side effect of obesity. Antioxidant activity from plant components regulates at some extent this imbalance. Main goal of the present study was to determine the antioxidant activity and protection against oxidative-induced damage of Acacia shaffneri (AS) and Acacia farnesiana (AF) pods extracts.

Methods

To evaluated antioxidant activity and radical scavenging capacity of AS and AF extracts, two experiments were performed: 1) pods extracts were challenged against H₂O₂ using kidney cells in an in vitro assay; and 2) (Meriones unguiculatus) was employed in an in vivo assay to observe the effect of pods extracts on scavenging properties in plasma.

Results

Both pods extracts presented an important protective effect on radical scavenging capacity against ABTS• + and DPPH⁺, and also in TBARS formation in vitro. Vegetal pods extracts did not induce any pro-oxidative effect when added to kidney cells in DMEM. Cells damage in DMEM with addition of H₂O₂ was significantly higher than those when vegetal pods extracts were added at 50 (P < 0.05) or 200 ppm (P < 0.001). Plasma scavenging properties presented an important dose-dependent positive effect in those groups where pods extracts were administered.

Conclusions

The antioxidant protection of the acacia pods extracts reported in this study suggests the possible transference of antioxidant components and protective effects to animal products (milk, meat, and by-products) from Acacia pods when this vegetation is included in the diet. In order to evaluate, the possible transference of theirs antioxidant components to animal products, the incorporation of these non-conventional resources to ruminant feeding is a good opportunity of study. Profiling of Acacia farnesiana pods extract is necessary to identify the responsible bioactive compounds of protective properties.

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Title: Antioxidant activity and protection against oxidative-induced damage of Acacia shaffneri and Acacia farnesiana pods extracts: in vitro and in vivo assays
Authors: Claudia Delgadillo Puga
Mario Cuchillo Hilario
José Guillermo Espinosa Mendoza
Omar Medina Campos
Eduardo Molina Jijón
Margarita Díaz Martínez
Marsela Alejandra Álvarez Izazaga
José Ángel Ledesma Solano
José Pedraza Chaverri
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2015
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-015-0959-y

At a glance: The STEP trials

Springer Medicine

Antioxidant activity and protection against oxidative-induced damage of Acacia shaffneri and Acacia farnesiana pods extracts: in vitro and in vivo assays

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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