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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2018

Open Access 01-12-2018 | Research article

Antimicrobial and antioxidant activities of triterpenoid and phenolic derivatives from two Cameroonian Melastomataceae plants: Dissotis senegambiensis and Amphiblemma monticola

Authors: Raissa Tioyem Nzogong, Fabrice Sterling Tchantchou Ndjateu, Steve Endeguele Ekom, Jules-Arnaud Mboutchom Fosso, Maurice Ducret Awouafack, Mathieu Tene, Pierre Tane, Hiroyuki Morita, Muhammad Iqbal Choudhary, Jean-de-Dieu Tamokou

Published in: BMC Complementary Medicine and Therapies | Issue 1/2018

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Abstract

Background

Antimicrobial resistance is a serious threat against humankind and the search for new therapeutics is needed. This study aims to investigate the antimicrobial and antioxidant activities of ethanol extracts and compounds isolated from Dissotis senegambiensis and Amphiblemma monticola, two Cameroonian Melastomataceae species traditionally used for the treatment of fever, malaria and infectious diseases.

Methods

The plant extracts were prepared by maceration in ethanol. Standard chromatographic and spectroscopic methods were used to isolate and identify fourteen compounds from the two plant species [1–6 (from D. senegambiensis), 3, 4 and 7–14 (from A. monticola)]. A two-fold serial micro-dilution method was used to determine the minimum inhibitory concentration (MIC) against four bacterial strains including two resistant bacterial strains, methicillin resistant S. aureus (MRSA3) and methicillin resistant S. aureus (MRSA4) and three yeast strains.

Results

The fractionation of EtOH extracts afforded fourteen compounds belonging to triterpenoid and phenolic derivatives. The ethanol extracts, compounds 3, 5–8, 10 and the mixture of 10 + 12 were active against all the tested bacterial and fungal species. Compound 7 (MIC = 16–32 μg/mL) and 10 (MIC = 8–16 μg/mL) displayed the largest antibacterial and antifungal activities, respectively. Compounds 7, 10 and the mixture of 10 + 12 showed prominent antibacterial activity against methicillin- resistant S. aureus (MRSA) which is in some cases equal to that of ciprofloxacin used as reference antibacterial drug. Compound 8 also showed high radical-scavenging activities and ferric reducing power when compared with vitamin C and butylated hydroxytoluene used as reference antioxidants. The tested samples were non-toxic to normal cells highlighting their good selectivity.

Conclusions

The result of this investigation reveals the potential of D. senegambiensis and A. monticola as well as the most active compounds in the search for new antimicrobial and antioxidant agents. So, further investigations are needed.
Appendix
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Literature
1.
Iwu MM, Duncan AR, Okunji CO. New antimicrobials of plant origin. In: Janick J, editor. Perspectives on new crops and new uses. Alexandria: ASHS Press; 1999.
2.
Mahady GB, Huang Y, Doyle BJ, Locklear T. Natural products as antibacterial agents. Nat Prod Chem. 2008;35:423–44.
3.
Tatsimo NSJ, Tamokou JD, Lamshöft M, Mouafo TF, Lannang MA, Sarkar P, et al. LC-MS guided isolation of antibacterial and cytotoxic constituents from Clausena anisata. Med Chem Res. 2015;24(4):1468–79.CrossRef
4.
Pagning NAL, Tamokou JD, Lateef M, Tapondjou AL, Kuiate JR, Ngnokam D, et al. New triterpene and new flavone glucoside from Rhynchospora corymbosa (Cyperaceae) with their antimicrobial, tyrosinase and butyrylcholinesterase inhibitory activities. Phytochem Lett. 2016;16(1):121–8.CrossRef
5.
Tebou PLF, Tamokou JD, Ngnokam D, Voutquenne-Nazabadioko L, Kuiate JR, Bag PK. Flavonoids from Maytenus buchananii as potential cholera chemotherapeutic agents. South Afri J Bot. 2017;109:58–65.CrossRef
6.
Loigier HA. Descriptive flora of Puerto Rico and adjacent islands, Spermatophyta: Cyrillaceae to Myrtaceae, vol 3. Puerto Rico: University of Puerto Rico Press; 1994, p 462.
7.
Maluma V. Les antherotomadissotis (inl. heterotis), Melastomataceae endémiques d’Afrique Centrale. Revue de Taxonomie et de Nomenclature Botaniques; 2005. p. 1–18.
8.
Tchebemou BB, Nganso DYO, Soh D, Zondegoumba NTE, Toghueo KRM, Sidjui SL, et al. Chemical constituents of Dissotis perkinsiae (Melastomaceae) and their antimicrobial activity. J Appl Pharm Sci. 2016;6:96–101.
9.
Bellakhdar J. La pharmacopée Marocaine traditionnelle: Médecine arabe ancienne et savoirs populaires, vol. 1. Paris-Rabat: Ibis Press-Eds Le Fennec; 1997. p 764.
10.
Cheek M, Woodgyer EM. New data on Amphiblemma monticola Jacq.-Fél. (Melastomataceae) from western Cameroon. Kew Bull. 2006;61:601–4.
11.
Jacques-Félix H. Le genre Amphiblemma Naud.(Melastomatacées). Adansonia. 1973;2:429–59.
12.
Jacques-Félix H. Un Amphiblemma (Melastomataceae) nouveau du Cameroun. Bulletin du Muséum National d'Histoire Naturelle, Section B. Adansonia. 1987;9:125–7.
13.
Ndjateu FST, Tene M, Tane P, Choudhary MI. A new C-methyl isoflavone and other compounds from the roots of Amphiblemma monticola (Melastomataceae). Nat Prod Commun. 2017;12:1731–2.
14.
Calderon AI, Terreaux C, Schenk K, Pattison P, Burdette JE, Pezzuto JM, et al. Isolation and structure elucidation of an isoflavone and a sesterterpenoic acid from Henriettella fascicularis. J Nat Prod. 2002;12:1749–53.CrossRef
15.
Zhang Z, Elsohly HN, Li XC, Khan SI, Broedel SE Jr, Raulli RE, et al. Flavanone glycosides from Miconia trailli. J Nat Prod. 2003;66:39–41.CrossRefPubMed
16.
Ndjateu FST, Tsafack RBN, Nganou BK, Awouafack MD, Wabo HK, Tene M, et al. Antimicrobial and antioxidant activities of extracts and ten compounds from three Cameroonian medicinal plants: Dissotis perkinsiae (Melastomataceae), Adenocarpus mannii (Fabaceae) and Barteria fistulosa (Passifloraceae). South Afri J Bot. 2014;91:37–42.CrossRef
17.
Nono RN, Barboni L, Teponno RB, Quassinti L, Bramucci M, Vitali LA, et al. Antimicrobial, antioxidant, anti-inflammatory activities and phytoconstituents of extracts from the roots of Dissotis thollonii Cogn. (Melastomataceae). South Afri J Bot. 2014;93:19–26.CrossRef
18.
Serna DM, Martinez JH. Phenolics and polyphenolics from Melastomataceae species. Molecules. 2015;20:17818–47.CrossRefPubMed
19.
Evans WC. Trease and Evans Pharmacognosy. 13th ed. Traiadal, London: Bailere; 1989.
20.
Harborne JB. Phytochemical methods: a guide to modern techniques of plant analysis. 2nd ed. London: Chapman and Hall Publishers; 1998.
21.
Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: a review. Int Pharm Sci. 2011;1:98–106.
22.
Banu S, Cathrine L. General techniques involved in phytochemical analysis. Int J Adv Res Chem Sci. 2015;2:25–32.
23.
Tsemeugne J, Sopbué FE, Tamokou JD, Tonle I, Kengne IC, Ngongang DA, et al. Electrochemical behavior and in vitro antimicrobial screening of some thienylazoaryls dyes. Chem Cent J. 2017;11:119.CrossRefPubMedPubMedCentral
24.
Tamokou JD, Tala FM, Wabo KH, Kuiate JR, Tane P. Antimicrobial activities of methanol extract and compounds from stem bark of Vismia rubescens. J Ethnopharmacol. 2009;124:571–5.CrossRef
25.
Padmaja M, Sravanthi M, Hemalatha KPJ. Evaluation of antioxidant activity of two Indian medicinal plants. J phytol. 2011;3:86–91.
26.
Djouossi MG, Tamokou JD, Ngnokam D, Kuiate JR, Tapondjou AL, Harakat D, et al. Antimicrobial and antioxidant flavonoids from the leaves of Oncoba spinosa Forssk. (Salicaceae). BMC Compl Altern Med. 2015;15:134.
27.
Situ H, Bobek LA. In vitro assessment of antifungal therapeutic potential of salivary histatin-5, two variants of histatin-5, and salivary mucin (MUC7) domain 1. Antimicrob Agents Chemother. 2000;44:1485–93.CrossRefPubMedPubMedCentral
28.
Marizeth LB, Jorge MD, Pedro Ade PP, Maria LSG, Juceni PD. Fatty acid esters of triterpenes from Erythroxylum passerinum. J Braz Chem Soc. 2002;13:669–73.CrossRef
29.
Nkeoma NO, Daniel LA, Henry NO, Emmanuel EI, Chukwuemeka SN, Festus BCO. Beta-Amyrin and alpha-amyrin acetate isolated from the stem bark of Alstonia boonei display profound anti-inflammatory activity. Pharm Biol. 2014;52:1478–86.CrossRef
30.
Seebacher W, Simic N, Weis R, Saf R, Kunert O. Complete assignments of 1H and 13C NMR resonances of oleanolic acid, 18α-oleanolic acid, ursolic acid and their 11- oxo derivatives. Magn Reson Chem. 2003;41:636–8.CrossRef
31.
Tene M, Tane P, Tamokou JD, Kuiate JR, Connolly JD. Degraded diterpenoids from the stem bark of Neoboutonia mannii. Phytochem Lett. 2008;1:120–4.CrossRef
32.
Ping W, Huiying H, Ruwei W, Naili W, Xinsheng Y. C glycosylfavones and aromatic glycosides from Campylotropis hirtella (Franch.) Schindl. Asian J Trad Med. 2007;2:149–53.
33.
Timmers M, Urban S. On-line (HPLC-NMR) and off-line phytochemical profiling of the Australian plant Lasiopetalum macrophyllum. Nat Prod Commun. 2011;7:551–60.
34.
Zhang F, Fu T-J, Peng S-L, Liu Z-R, Ding L-S. Two new triterpenoids from the roots of Sanguisorba officinalis L. J Integr Plant Biol. 2005;47:251–6.CrossRef
35.
Alam A, Takaguchi Y, Tsuboi S. Synthesis of ellagic acid and its 4,4′-di-O-alkyl derivatives from gallic acid. J Fac environ Sci Technol. Okayama University. 2005;10:111–7.
36.
Wang C-M, Chen H-T, Wu Z-Y, Jhan Y-L, Shyu C-L, Chou C-H. Antibacterial and synergistic activity of pentacyclic triterpenoids isolated from Alstonia scholaris. Molecules. 2016;21(2):139.CrossRefPubMed
37.
38.
Yang X-H, Guo Y-W. Two new ellagic acid glycosides from leaves of Diplopanax stachyanthus. J Asian Nat Prod Res. 2004;6:271–6.CrossRef
39.
Reuben KD, Abdulrahman FI, Akan JC, Usman H, Sodipo OA, Egwu GO. Phytochemical screening and in vitro antimicrobial investigation of the methanolic extract of Croton Zambesicus Muell ARG. Stem bark. Eur J Sci Res. 2008;23(1):134–40.
40.
Tamokou JD, Mbaveng TA, Kuete V. Antimicrobial activities of African medicinal spices and vegetables. In: Medicinal spices and vegetables from Africa: therapeutic potential against metabolic, inflammatory, infectious and systemic diseases. 1st ed: Elsevier; 2017, Chapter 8. p. 207–37.
41.
Tene M, Ndontsa LB, Tane P, Tamokou JD, Kuiate JR. Antimicrobial diterpenoids and triterpenoids from the stem bark of Croton macrostachys. Int J Biol Chem Sci. 2009;3:538–44.
42.
Chouna JR, Tamokou JD, Nkeng-Efouet-Alango P, Lenta NB, Sewald N. Antimicrobial triterpenes from the stem bark of Crossopteryx febrifuga. Zeitschrift für Naturforschung C. 2015;70(7–8):c:169–73.CrossRef
43.
44.
Tatsimo NSJ, Tamokou JD, Havyarimana L, Dezso C, Forgo P, Hohmann J, et al. Antimicrobial and antioxidant activity of kaempferol rhamnoside derivatives from Bryophyllum pinnatum. BMC Res Notes. 2012;5:158.CrossRefPubMedPubMedCentral
45.
Scalbert A. Antimicrobial properties of tannins. Phytochemistry. 1991;30:3875–83.CrossRef
46.
47.
Olayinka AA, Anthony IO. Preliminary phytochemical screening and in vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. BMC Complement Altern Med. 2010;10:21.CrossRef
48.
Li Y, Guo C, Yang J, Wei J, Xu J, Cheng S. Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chem. 2006;96(2):254–60.CrossRef
49.
Karou D, Nadembega WMC, Ouattara L, Ilboudo DP, Canini A, Nikiéma JB, et al. African ethnopharmacology and new drug discovery. Med Aromat Plant Sci Biotechnol. 2007;1(1):1–9.
50.
Pietta P, Sionetti P, Mauri P. Antioxidant activity of selected medicinal plants. J Agric Food Chem. 1998;46:4487–90.CrossRef
Metadata
Title
Antimicrobial and antioxidant activities of triterpenoid and phenolic derivatives from two Cameroonian Melastomataceae plants: Dissotis senegambiensis and Amphiblemma monticola
Authors
Raissa Tioyem Nzogong
Fabrice Sterling Tchantchou Ndjateu
Steve Endeguele Ekom
Jules-Arnaud Mboutchom Fosso
Maurice Ducret Awouafack
Mathieu Tene
Pierre Tane
Hiroyuki Morita
Muhammad Iqbal Choudhary
Jean-de-Dieu Tamokou
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2018
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-018-2229-2

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