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Published in: BMC Complementary Medicine and Therapies 1/2010

Open Access 01-12-2010 | Research article

Antidiarrhoea and toxicological evaluation of the leaf extract of Dissotis rotundifolia triana (Melastomataceae)

Authors: Tavs A Abere, Pius E Okoto, Freddy O Agoreyo

Published in: BMC Complementary Medicine and Therapies | Issue 1/2010

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Abstract

Background

The leaves of Dissotis rotundifolia are used ethnomedically across Africa without scientific basis or safety concerns. Determination of its phytochemical constituents, antimicrobial activity, effects on the gastrointestinal tract (GIT) as well as toxicological profile will provide supportive scientific evidence in favour of its continous usage.

Method

Chemical and chromatographic tests were employed in phytochemical investigations. Inhibitory activity against clinical strains of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella typhi were compared with Gentamycin. Our report includes minimum inhibitory concentration (MIC) against the tested organisms. The effect of the ethanol extract on the motility of the GIT in mice using the charcoal plug method and castor oil induced diarrhoea in rats was evaluated. Toxicological evaluation was determined by administering 250 mg/kg and 500 mg/kg of extracts on male Wistar rats for 14 days with normal saline as control. The tissues of the kidneys, liver, heart and testes were examined.

Results

Phytochemical studies revealed the presence of alkaloids, saponin and cardiac glycosides. The crude ethanol extract and fractions inhibited the growth of E. coli, P. aeruginosa, S. aureus and S. typhi to varying extents. The degree of transition exhibited by the charcoal meal was dose-dependent. In the castor oil induced diarrhoea test, all the doses showed anti-spasmodic effects. The LD50 in mice was above 500 mg/kg body weight. Toxicological evaluation at 500 mg/kg showed increased cytoplasmic eosinophilia and densely stained nuclei of the liver, tubular necrosis of the kidney, presence of ill-defined testes with indistinct cell outlines and no remarkable changes in the heart.

Conclusion

Ethanol extracts of Dissotis rotundifolia have demonstrated antimicrobial activity against clinical strains of selected microorganisms. The plant showed potential for application in the treatment of diarrhoea, thereby justifying its usage across Africa. It also demonstrated toxicity in certain organs at the dose of 500 mg/kg, and it will be necessary to fully establish its safety profile.
Literature
1.
go back to reference Srivastera J, Lambert J, Vietweyar N: Medicinal plants: An expanding role in development. World Bank Technical Paper. 1996, 320 (1): Srivastera J, Lambert J, Vietweyar N: Medicinal plants: An expanding role in development. World Bank Technical Paper. 1996, 320 (1):
2.
go back to reference Baurmman RH: Traditional Medicine in Modern Healthcare. World Health Forum. 1982, 3 (1): Baurmman RH: Traditional Medicine in Modern Healthcare. World Health Forum. 1982, 3 (1):
4.
go back to reference Eisenberg DM, Kester RC, Foster C, Norlock FE, Calkins DR, Delbanco TL: Unconventional medicine in the United States: prevalence, costs and patterns of use. N Engl J Med. 1993, 328: 246-52. 10.1056/NEJM199301283280406.CrossRefPubMed Eisenberg DM, Kester RC, Foster C, Norlock FE, Calkins DR, Delbanco TL: Unconventional medicine in the United States: prevalence, costs and patterns of use. N Engl J Med. 1993, 328: 246-52. 10.1056/NEJM199301283280406.CrossRefPubMed
5.
go back to reference Eisenberg DM, Davis RB, Ettner SC, Appel S, Wilkey S, VanRompay M, Kessler R: Trends in alternative medicine use in the United States, 1990-1997: results of a follow-up survey. JAMA. 1998, 280: 1569-75. 10.1001/jama.280.18.1569.CrossRefPubMed Eisenberg DM, Davis RB, Ettner SC, Appel S, Wilkey S, VanRompay M, Kessler R: Trends in alternative medicine use in the United States, 1990-1997: results of a follow-up survey. JAMA. 1998, 280: 1569-75. 10.1001/jama.280.18.1569.CrossRefPubMed
6.
go back to reference Sofowora A: The state of medicinal plants research in Nigeria. 1986, Ibadan University press, Nigeria, 101-1 Sofowora A: The state of medicinal plants research in Nigeria. 1986, Ibadan University press, Nigeria, 101-1
7.
go back to reference Loigier HA: Spermatophyta. Descriptive flora of Puerto Rico and Adjacent Islands. 1994, 1: 3- Loigier HA: Spermatophyta. Descriptive flora of Puerto Rico and Adjacent Islands. 1994, 1: 3-
8.
go back to reference Wagner WL, Herbst DR, Sohmer SH: Manual of the flowering plants of Hawaii. 1990, University of Hawaii press, Bishop Museum, Honolulu, 210- Wagner WL, Herbst DR, Sohmer SH: Manual of the flowering plants of Hawaii. 1990, University of Hawaii press, Bishop Museum, Honolulu, 210-
9.
go back to reference Gill LS: Ethnomedical uses of plants in Nigeria. 1992, University of Benin press, 103- Gill LS: Ethnomedical uses of plants in Nigeria. 1992, University of Benin press, 103-
11.
go back to reference Kokwaso JC: Medicinal plants of East Africa. 1976, General printers, Nairobi, Kenya, 198- Kokwaso JC: Medicinal plants of East Africa. 1976, General printers, Nairobi, Kenya, 198-
12.
go back to reference Noumi E, Yomi A: Medicinal plants used in intestinal diseases in Mbalmayo Region. Fitoterapia. 2001, 246-254. 10.1016/S0367-326X(00)00288-4. Noumi E, Yomi A: Medicinal plants used in intestinal diseases in Mbalmayo Region. Fitoterapia. 2001, 246-254. 10.1016/S0367-326X(00)00288-4.
13.
go back to reference Watt JM, Breyer-Brandwijk MG: The medicinal and poisonous plants of Southern and Eastern Africa. 1962, E & S Livingstone Ltd, Edinburgh & London, 137: 744-2 Watt JM, Breyer-Brandwijk MG: The medicinal and poisonous plants of Southern and Eastern Africa. 1962, E & S Livingstone Ltd, Edinburgh & London, 137: 744-2
14.
go back to reference Brain KR, Turner TD: Practical evaluation of Phytopharmaceuticals. 1975, Wright-Scientechnica, Bristol, 144-1 Brain KR, Turner TD: Practical evaluation of Phytopharmaceuticals. 1975, Wright-Scientechnica, Bristol, 144-1
15.
go back to reference Ciulei I: Methodology for analysis of vegetable drugs. UNIDO Romania. 1981, 17-25. Ciulei I: Methodology for analysis of vegetable drugs. UNIDO Romania. 1981, 17-25.
16.
go back to reference Harborne JB: Phytochemical methods. A guide to modern technique of plant analysis. 1992, Chapman and Hill, London, 279- Harborne JB: Phytochemical methods. A guide to modern technique of plant analysis. 1992, Chapman and Hill, London, 279-
17.
go back to reference Evans WC: Trease and Evans Pharmacognosy. 1996, WB Saunders Ltd. London, 119-159. 14 Evans WC: Trease and Evans Pharmacognosy. 1996, WB Saunders Ltd. London, 119-159. 14
18.
go back to reference Onwukaeme ND, Anuforo DC: Phytochemical and Pharmacological studies on Pycanthus angolensis (Welb) Warb (Myristicaceae). Discovery and Innovation. 1993, 5: 317-322. Onwukaeme ND, Anuforo DC: Phytochemical and Pharmacological studies on Pycanthus angolensis (Welb) Warb (Myristicaceae). Discovery and Innovation. 1993, 5: 317-322.
19.
go back to reference Izzo AA, Nicoleti M, Giannatassio B, Capasso F: Antidiarrhoeal activity of Terminalia sevicea Burch. Ex. Dc. Extracts. Natural Drugs and the Digestive tract EMSI, Rome. Edited by: Capasso F, Mascolo N. 1992, 223-230. Izzo AA, Nicoleti M, Giannatassio B, Capasso F: Antidiarrhoeal activity of Terminalia sevicea Burch. Ex. Dc. Extracts. Natural Drugs and the Digestive tract EMSI, Rome. Edited by: Capasso F, Mascolo N. 1992, 223-230.
20.
go back to reference Washington JA: In manual of clinical microbiology. Edited by: Lennette E, Balowa A, Hausler WJ, Shadomy HJ. 1985, American Society of Microbiology, Washinggton DC, 967-970. Washington JA: In manual of clinical microbiology. Edited by: Lennette E, Balowa A, Hausler WJ, Shadomy HJ. 1985, American Society of Microbiology, Washinggton DC, 967-970.
21.
go back to reference Dietrich L: A new approach to practical acute toxicity testing. Archives of Toxicology. 1983, 54: 275-287. 10.1007/BF01234480.CrossRef Dietrich L: A new approach to practical acute toxicity testing. Archives of Toxicology. 1983, 54: 275-287. 10.1007/BF01234480.CrossRef
22.
go back to reference Adefemi OA, Elujoba AA, Odesanmi WO: Evaluation of the toxicity potential of Cassia podocarpa with reference to official Senna. West Afr Journal of Pharmacol and Drug Res. 1988, 8: 41-48. Adefemi OA, Elujoba AA, Odesanmi WO: Evaluation of the toxicity potential of Cassia podocarpa with reference to official Senna. West Afr Journal of Pharmacol and Drug Res. 1988, 8: 41-48.
23.
go back to reference Obasi NB, Igboechi AC, Anuforo DC: Studies of the antidiarrhoeal potentials of some compositae plants. Medicinal plants in a developing economy. Proceedings of a workshop organized by the Nigerian Society of Nigeria. 1990, 108-116. Obasi NB, Igboechi AC, Anuforo DC: Studies of the antidiarrhoeal potentials of some compositae plants. Medicinal plants in a developing economy. Proceedings of a workshop organized by the Nigerian Society of Nigeria. 1990, 108-116.
24.
go back to reference Abere TA, Agoreyo FO: Antimicrobial and toxicological evaluation of the leaves of Baissea axillaries Hua used in the management of HIV/AIDS patients. BMC Complement Altern Med. 2006, 6: 22-10.1186/1472-6882-6-22.CrossRefPubMedPubMedCentral Abere TA, Agoreyo FO: Antimicrobial and toxicological evaluation of the leaves of Baissea axillaries Hua used in the management of HIV/AIDS patients. BMC Complement Altern Med. 2006, 6: 22-10.1186/1472-6882-6-22.CrossRefPubMedPubMedCentral
25.
go back to reference Klein R: Toxicology and herbs. Aust Journal of Med Herbalism. 1996, 8: 100-110. Klein R: Toxicology and herbs. Aust Journal of Med Herbalism. 1996, 8: 100-110.
26.
go back to reference Akubue PI: Textbook of Pharmacology. 2006, African First Publishers Limited, 30-31. 1 Akubue PI: Textbook of Pharmacology. 2006, African First Publishers Limited, 30-31. 1
27.
go back to reference Malmfors T, Teiling A: LD 50: Its value for the pharmaceutical industry in safety evaluation of drugs. Acta Pharmacol Toxicol. 1983, 52 (Suppl 2): 229- Malmfors T, Teiling A: LD 50: Its value for the pharmaceutical industry in safety evaluation of drugs. Acta Pharmacol Toxicol. 1983, 52 (Suppl 2): 229-
Metadata
Title
Antidiarrhoea and toxicological evaluation of the leaf extract of Dissotis rotundifolia triana (Melastomataceae)
Authors
Tavs A Abere
Pius E Okoto
Freddy O Agoreyo
Publication date
01-12-2010
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2010
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/1472-6882-10-71

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