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01-06-2018 | Laboratory Investigation

Anti-differentiation non-coding RNA, ANCR, is differentially expressed in different types of brain tumors

Authors: Mahshid Malakootian, Fatemeh Mirzadeh Azad, Youssef Fouani, Elham Taheri Bajgan, Hooshang Saberi, Seyed Javad Mowla

Published in: Journal of Neuro-Oncology | Issue 2/2018

Abstract

Long non-coding RNAs (lncRNAs) are important modulators of various cellular and molecular events, including cancer-associated pathways. The Anti-differentiation ncRNA (ANCR) is a key regulator of keratinocyte differentiation, where its expression is necessary to maintain epidermal progenitor’s cells. Herein, we investigated the expression pattern of ANCR in the course of neural differentiation. Moreover, we used published RNAseq data and clinical samples to evaluate the alteration of ANCR expression in different cell types and brain tumors. Furthermore, we manipulated ANCR expression in glioma cell lines to clarify a potential functional role for ANCR in tumorigenesis. Our qRT-PCR results revealed a significant upregulation of ANCR in more malignant and less differentiated types of brain tumors (P = 0.03). This data was in accordance with down regulation of ANCR during neural differentiation. ANCR suppression caused an elevation in apoptosis rate, as well as a G1 cell cycle arrest in glioblastoma cell line. Altogether, our data demonstrated that ANCR may play a role in glioma genesis and that it could be considered as a potential diagnostic and therapeutic target to combat brain cancers.

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Title: Anti-differentiation non-coding RNA, ANCR, is differentially expressed in different types of brain tumors
Authors: Mahshid Malakootian
Fatemeh Mirzadeh Azad
Youssef Fouani
Elham Taheri Bajgan
Hooshang Saberi
Seyed Javad Mowla
Publication date: 01-06-2018
Publisher: Springer US
Published in: Journal of Neuro-Oncology / Issue 2/2018
Print ISSN: 0167-594X
Electronic ISSN: 1573-7373
DOI: https://doi.org/10.1007/s11060-018-2809-5

At a glance: The STEP trials

Springer Medicine

Anti-differentiation non-coding RNA, ANCR, is differentially expressed in different types of brain tumors

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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