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Inflammation Research
Published in: Inflammation Research 1/2013

01-01-2013 | Original Research Paper

Anti-arthritic activity of the Indian leafy vegetable Cardiospermum halicacabum in Wistar rats and UPLC–QTOF–MS/MS identification of the putative active phenolic components

Authors: Ramachandran Jeyadevi, Thilagar Sivasudha, Angappan Rameshkumar, Lakshmanan Dinesh Kumar

Published in: Inflammation Research | Issue 1/2013

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Abstract

Objectives

The present work was carried out to investigate the free radical scavenging activity of the ethanol extract of C. halicacabum leaves (EECH), to study its antioxidant properties and anti-rheumatic effects in Wistar rats with CFA-induced arthritis, and to profile the phenolic components thereof by LC–MS/MS.

Methods

The free radical scavenging activities of the extract was evaluated by NO and superoxide anion scavenging assays. Arthritis was induced to the albino Wistar rats by CFA. Fifteen days after CFA induction, arthritic rats received EECH orally at the doses of 250 and 500 mg/kg daily for 20 days. Diclofenac sodium was used as reference standard. EECH is subjected to LC–MS/MS analysis for the identification of phenolic compounds.

Results

The IC50 value of the EECH to scavenge the NO and superoxide radicals are 83 and 60 μg/ml respectively. Ultrasonography and histology images of hind limb in EECH treated groups confirmed the complete cartilage regeneration. The LC/MS/MS analysis indicated the presence of anti-inflammatory compounds luteolin-7-O-glucuronide, apigenin-7-O-glucuronide and chrysoeriol.

Conclusion

These findings lend pharmacological support to the reported folkloric use of C. halicacabum in the treatment and management of painful, arthritic inflammatory conditions.
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Metadata
Title
Anti-arthritic activity of the Indian leafy vegetable Cardiospermum halicacabum in Wistar rats and UPLC–QTOF–MS/MS identification of the putative active phenolic components
Authors
Ramachandran Jeyadevi
Thilagar Sivasudha
Angappan Rameshkumar
Lakshmanan Dinesh Kumar
Publication date
01-01-2013
Publisher
SP Birkhäuser Verlag Basel
Published in
Inflammation Research / Issue 1/2013
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-012-0558-z

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