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Open Access 01-12-2018 | Research article

Angiogenesis and radiological tumor growth in patients with glioblastoma

Authors: Vilde Elisabeth Mikkelsen, Anne Line Stensjøen, Unn Sophie Granli, Erik Magnus Berntsen, Øyvind Salvesen, Ole Solheim, Sverre Helge Torp

Published in: BMC Cancer | Issue 1/2018

Abstract

Background

The preoperative growth of human glioblastomas (GBMs) has been shown to vary among patients. In animal studies, angiogenesis has been linked to hypoxia and faster growth of GBM, however, its relation to the growth of human GBMs is sparsely studied. We have therefore aimed to look for associations between radiological speed of growth and microvessel density (MVD) counts of the endothelial markers vWF (Factor VIII related antigen) and CD105 (endoglin).

Methods

Preoperative growth was estimated from segmented tumor volumes of two preoperative T1-weighted postcontrast magnetic resonance imaging scans taken ≥14 days apart in patients with newly diagnosed GBMs. A Gompertzian growth curve was computed from the volume data and separated the patients into two groups of either faster or slower tumor growth than expected. MVD counts of the immunohistochemical markers von Willebrand factor (vWF) (a pan-endothelial marker) and CD105 (a marker of proliferating endothelial cells) were assessed for associations with fast-growing tumors using Mann-Whitney U tests and a multivariable binary logistic regression analysis.

Results

We found that only CD105-MVD was significantly associated with faster growth in a univariable analysis (p = 0.049). However, CD105-MVD was no longer significant when corrected for the presence of thromboses and high cellular density in a multivariable model, where the latter features were significant independent predictors of faster growth with respective odds ratios 4.2 (95% confidence interval, 1.2, 14.3), p = 0.021 and 2.6 (95% confidence interval, 1.0, 6.5), p = 0.048.

Conclusions

MVDs of neither endothelial marker were independently associated with faster growth, suggesting angiogenesis-independent processes contribute to faster glioblastoma growth.

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Title: Angiogenesis and radiological tumor growth in patients with glioblastoma
Authors: Vilde Elisabeth Mikkelsen
Anne Line Stensjøen
Unn Sophie Granli
Erik Magnus Berntsen
Øyvind Salvesen
Ole Solheim
Sverre Helge Torp
Publication date: 01-12-2018
Publisher: BioMed Central
Published in: BMC Cancer / Issue 1/2018
Electronic ISSN: 1471-2407
DOI: https://doi.org/10.1186/s12885-018-4768-9

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