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Open Access 01-12-2020 | Anesthetics | Research article

Maternal sciatic nerve administered bupivacaine induces hippocampal cell apoptosis in offspring

Authors: Alireza Mirkheshti, Alireza Shakeri, Elham Memary, Mansoureh Baniasadi, Jalal Zaringhalam, Ardeshir Tajbakhsh, Marzieh Mirzaei, Elena Lak

Published in: BMC Anesthesiology | Issue 1/2020

Abstract

Background

Bupivacaine, an amid-type local anesthetic, is widely used for clinical patients especially in pregnant women. In addition to neurotoxicity effect of bupivacaine, it can cross the placenta, accumulates in this tissue and retained in fetal tissues. Nevertheless, whether bupivacaine can cause neurotoxicity in fetus remains unclear. Hence, this study was design to investigate the effects of maternal bupivacaine use on fetus hippocampal cell apoptosis and the possible related mechanism.

Methods

On day 15 of pregnancy, sciatic nerve of pregnant wistar rat (180–200 g) were exposed by lateral incision of the right thigh and 0.2 ml of bupivacaine was injected. After their delivery, we randomly selected one male offspring of every mother. On day 30 after of their birth, the rat’s hippocampi were isolated for molecular studies. Western blotting was used to examine the expression of cleaved caspase-3, caspase-8 and p-Akt in fetal hippocampus.

Results

Our results showed that maternal bupivacaine use caused a significant increment of cleaved caspase-3 and caspase-8 expression in fetal hippocampus compared with the sham group. In addition, maternally administered bupivacaine could significantly decrease hippocampal P.Akt/T.Akt ratio which was concurrent with an increment of cleaved caspase-3 and caspase-8 expression.

Conclusion

Our data suggest that maternal bupivacaine use increases fetal hippocampal cell apoptosis markers such as caspase 8 and cleaved caspase 3, at least in part, via inhibiting the Akt activation.

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Title: Maternal sciatic nerve administered bupivacaine induces hippocampal cell apoptosis in offspring
Authors: Alireza Mirkheshti
Alireza Shakeri
Elham Memary
Mansoureh Baniasadi
Jalal Zaringhalam
Ardeshir Tajbakhsh
Marzieh Mirzaei
Elena Lak
Publication date: 01-12-2020
Publisher: BioMed Central
Keywords: Anesthetics
Anesthetics
Published in: BMC Anesthesiology / Issue 1/2020
Electronic ISSN: 1471-2253
DOI: https://doi.org/10.1186/s12871-020-01143-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Maternal sciatic nerve administered bupivacaine induces hippocampal cell apoptosis in offspring

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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