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European Radiology
Published in: European Radiology 5/2018

01-05-2018 | Ultrasound

Anatomical Road Mapping Using CT and MR Enterography for Ultrasound Molecular Imaging of Small Bowel Inflammation in Swine

Authors: Huaijun Wang, Stephen A. Felt, Ismayil Guracar, Valentina Taviani, Jianhua Zhou, Rosa Maria Silveira Sigrist, Huiping Zhang, Joy Liau, José G. Vilches-Moure, Lu Tian, Yamil Saenz, Thierry Bettinger, Brian A. Hargreaves, Amelie M. Lutz, Jürgen K. Willmann

Published in: European Radiology | Issue 5/2018

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Abstract

Objectives

To evaluate the feasibility and time saving of fusing CT and MR enterography with ultrasound for ultrasound molecular imaging (USMI) of inflammation in an acute small bowel inflammation of swine.

Methods

Nine swine with ileitis were scanned with either CT (n = 3) or MR (n = 6) enterography. Imaging times to load CT/MR images onto a clinical ultrasound machine, fuse them to ultrasound with an anatomical landmark-based approach, and identify ileitis were compared to the imaging times without anatomical road mapping. Inflammation was then assessed by USMI using dual selectin-targeted (MBSelectin) and control (MBControl) contrast agents in diseased and healthy control bowel segments, followed by ex vivo histology.

Results

Cross-sectional image fusion with ultrasound was feasible with an alignment error of 13.9 ± 9.7 mm. Anatomical road mapping significantly reduced (P < 0.001) scanning times by 40%. Localising ileitis was achieved within 1.0 min. Subsequently performed USMI demonstrated significantly (P < 0.001) higher imaging signal using MBSelectin compared to MBControl and histology confirmed a significantly higher inflammation score (P = 0.006) and P- and E-selectin expression (P ≤ 0.02) in inflamed vs. healthy bowel.

Conclusions

Fusion of CT and MR enterography data sets with ultrasound in real time is feasible and allows rapid anatomical localisation of ileitis for subsequent quantification of inflammation using USMI.

Key Points

• Real-time fusion of CT/MRI with ultrasound to localise ileitis is feasible.
• Anatomical road mapping using CT/MRI significantly decreases the scanning time for USMI.
• USMI allows quantification of inflammation in swine, verified with ex vivo histology.
Appendix
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Metadata
Title
Anatomical Road Mapping Using CT and MR Enterography for Ultrasound Molecular Imaging of Small Bowel Inflammation in Swine
Authors
Huaijun Wang
Stephen A. Felt
Ismayil Guracar
Valentina Taviani
Jianhua Zhou
Rosa Maria Silveira Sigrist
Huiping Zhang
Joy Liau
José G. Vilches-Moure
Lu Tian
Yamil Saenz
Thierry Bettinger
Brian A. Hargreaves
Amelie M. Lutz
Jürgen K. Willmann
Publication date
01-05-2018
Publisher
Springer Berlin Heidelberg
Published in
European Radiology / Issue 5/2018
Print ISSN: 0938-7994
Electronic ISSN: 1432-1084
DOI
https://doi.org/10.1007/s00330-017-5148-6

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