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Virchows Archiv
Published in: Virchows Archiv 4/2011

01-04-2011 | Original Article

Analysis of select members of the E26 (ETS) transcription factors family in colorectal cancer

Authors: Candida Deves, Daiana Renck, Bernardo Garicochea, Vinicius Duval da Silva, Tiago Giulianni Lopes, Henrique Fillman, Lucio Fillman, Silvana Lunardini, Luis Augusto Basso, Diogenes Santiago Santos, Eraldo L. Batista Jr.

Published in: Virchows Archiv | Issue 4/2011

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Abstract

The E-twenty-six (ETS) family of transcription factors is known to act as positive or negative regulators of the expression of genes that are involved in diverse biological processes, including those that control cellular proliferation, differentiation, hematopoiesis, apoptosis, metastasis, tissue remodeling, and angiogenesis. Identification of target gene promoters of normal and oncogenic transcription factors provides new insights into the regulation of genes that are involved in the control of normal cell growth and differentiation. The aim of the present investigation was to analyze the differential expression of 11 ETS (ELF-3, ESE3, ETS1, ETV3, ETV4, ETV6, NERF, PDEF, PU1, Spi-B, and Spi-C) as potential markers for prognostic of colorectal cancer. A series of paired tissue biopsies consisting of a tumor and a non-affected control sample were harvested from 28 individuals suffering from diagnosed colorectal lesions. Total RNA was isolated from the samples, and after reverse transcription, differential expression of the select ETS was carried out through real-time polymerase chain reaction. Tumor staging as determined by histopathology was carried out to correlate the degree of tumor invasiveness with the expression of the ETS genes. The results demonstrated a different quantitative profile of expression in tumors and normal tissues. ETV4 was significantly upregulated with further increase in the event of lymph node involvement. PDEF and Spi-B presented downregulation, which was more significant when lymph node involvement was present. These findings were supported by immunohistochemistry of tumoral tissues. The results suggest that select ETS may serve as potential markers of colorectal cancer invasiveness and metastasis.
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Metadata
Title
Analysis of select members of the E26 (ETS) transcription factors family in colorectal cancer
Authors
Candida Deves
Daiana Renck
Bernardo Garicochea
Vinicius Duval da Silva
Tiago Giulianni Lopes
Henrique Fillman
Lucio Fillman
Silvana Lunardini
Luis Augusto Basso
Diogenes Santiago Santos
Eraldo L. Batista Jr.
Publication date
01-04-2011
Publisher
Springer-Verlag
Published in
Virchows Archiv / Issue 4/2011
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI
https://doi.org/10.1007/s00428-011-1053-6

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