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Head & Face Medicine
Published in: Head & Face Medicine 1/2009

Open Access 01-12-2009 | Research

Analysis of expression profiles of MAGE-A antigens in oral squamous cell carcinoma cell lines

Authors: Urs DA Müller-Richter, Albert Dowejko, Tobias Reuther, Johannes Kleinheinz, Torsten E Reichert, Oliver Driemel

Published in: Head & Face Medicine | Issue 1/2009

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Abstract

Background

The immunological response to solid tumours is insufficient. Therefore, tumour specific antigens have been explored to facilitate the activation of the immune system. The cancer/testis antigen class of MAGE-A antigens is a possible target for vaccination. Their differential expression profiles also modulate the course of the cancer disease and its response to antineoplastic drugs.

Methods

The expression profiles of MAGE-A2, -A3, -A4, -A6 and -A10 in five own oral squamous cell carcinoma cell lines were characterised by rt-PCR, qrt-PCR and immunocytochemistry with a global MAGE-A antibody (57B) and compared with those of an adult keratinocyte cell line (NHEK).

Results

All tumour cell lines expressed MAGE-A antigens. The antigens were expressed in groups with different preferences. The predominant antigens expressed were MAGE-A2, -A3 and -A6. MAGE-A10 was not expressed in the cell lines tested. The MAGE-A gene products detected in the adult keratinocyte cell line NHEK were used as a reference.

Conclusion

MAGE-A antigens are expressed in oral squamous cell carcinomas. The expression profiles measured facilitate distinct examinations in forthcoming studies on responses to antineoplastic drugs or radiation therapy. MAGE-A antigens are still an interesting aim for immunotherapy.
Appendix
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Metadata
Title
Analysis of expression profiles of MAGE-A antigens in oral squamous cell carcinoma cell lines
Authors
Urs DA Müller-Richter
Albert Dowejko
Tobias Reuther
Johannes Kleinheinz
Torsten E Reichert
Oliver Driemel
Publication date
01-12-2009
Publisher
BioMed Central
Published in
Head & Face Medicine / Issue 1/2009
Electronic ISSN: 1746-160X
DOI
https://doi.org/10.1186/1746-160X-5-10

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