Top

European Journal of Orthopaedic Surgery & Traumatology

Published in:

01-12-2014 | Original Article

An evaluation of the delayed effect of intra-articular injections of lidocaine (2 %) on articular cartilage: an experimental study in rabbits

Authors: Hamidreza Yazdi, Bahahreh Tabatabaeian Nimavard, Mohammadali Shokrgozar, Mohammadmehdi Dehghan, Reza Jamei Moayedi, Mohammad Majidi, Tahmineh Mokhtari

Published in: European Journal of Orthopaedic Surgery & Traumatology | Issue 8/2014

Abstract

Lidocaine is commonly injected into the joints as an analgesic. The aim of the present study was to evaluate the delayed effect of intra-articular injections of lidocaine (2 %) on articular cartilage in rabbit knees. Ten rabbits were divided into two groups, each group containing five animals. Two milliliters of normal saline solution was injected into both knee joints of animals in group one (control group), and 2 ml of lidocaine was injected into both knee joints of animals in group two (case group). After 8 weeks, the articular cartilage of the distal femur was harvested and analyzed through confocal microscopy and real-time polymerase chain reaction to evaluate the viability and function of chondrocytes, respectively. Confocal microscopy showed a significant decrease in the number of live cells caused by lidocaine (P ≤ 0.001). The changes in gene expression of collagen types II (COL II) and aggrecan were significant in group two (P = 0.008 and P = 0.002, respectively). According to the results, the delayed in vivo effect of lidocaine on chondrocyte is to reduce live chondrocytes and change in the gene expression of COL II and aggrecan.

Chu CR, Coyle CH, Chu CT, Szczodry M, Seshadri V, Karpie JC, Cieslak KM, Pringle EK (2010) In vivo effects of single intra-articular injection of 0.5% bupivacaine on articular cartilage. J Bone Joint Surg Am 92(3):599–608. doi:10.1007/BF02899701 PubMedCrossRef

Jacobs TF, Vansintjan PS, Roels N, Herregods SS, Verbruggen G, Herregods LI, Almqvist KF (2011) The effect of lidocaine on the viability of cultivated mature human cartilage cells: an in vitro study. Knee Surg Sports Traumatol Arthrosc 19(7):1206–1213. doi:10.1172/JCI106224 PubMedCrossRef

Karpie JC, Chu CR (2007) Lidocaine exhibits dose-and time-dependent cytotoxic effects on bovine articular chondrocytes in vitro. Am J Sports Med 35(10):1621–1627. doi:10.1302/0301-620x.90b6.20079 PubMedCrossRef

Petty DH, Jazrawi LM, Estrada LS, Andrews JR (2004) Glenohumeral chondrolysis after shoulder arthroscopy case reports and review of the literature. Am J Sports Med 32(2):509–515. doi:10.1302/0301-620x.90b6.20079 PubMedCrossRef

Chu C, Izzo N, Coyle C, Papas N, Logar A (2008) The in vitro effects of bupivacaine on articular chondrocytes. J Bone Joint Surg Br 90(6):814–820. doi:10.2106/jbjs.i.00425 PubMedCrossRef

Chu CR, Izzo NJ, Papas NE, Fu FH (2006) In vitro exposure to 0.5% bupivacaine is cytotoxic to bovine articular chondrocytes. Arthroscopy 22(7):693–699PubMedCrossRef

Gomoll AH, Kang RW, Williams JM, Bach BR, Cole BJ (2006) Chondrolysis after continuous intra-articular bupivacaine infusion: an experimental model investigating chondrotoxicity in the rabbit shoulder. Arthroscopy 22(8):813–819. doi:10.1177/147323000403200509 PubMedCrossRef

Bojescul JA, Wilson G, Taylor DC (2005) Idiopathic chondrolysis of the ankle. Arthroscopy 21(2):224–227. doi:10.1177/0363546508318190 PubMedCrossRef

Hansen BP, Beck CL, Beck EP, Townsley RW (2007) Postarthroscopic glenohumeral chondrolysis. J Am Acad Orthop Surg 35(10):1628–1634. http://http://jpet.aspetjournals.org/content/285/2/413.long. Accessed 23 Oct 2013

10.

Sanders TG, Zlatkin MB, Paruchuri NB, Higgins RW (2007) Chondrolysis of the glenohumeral joint after arthroscopy: findings on radiography and low-field-strength MRI. AJR Am J Roentgenol 188(4):1094–1098. doi:10.1016/j.arthro.2006.06.006 PubMedCrossRef

11.

Dragoo Jl, Korotkova T, Kanwar R, Wood B (2008) The effect of local anesthetics administered via pain pump on chondrocyte viability. Am J Sports Med 36(8):1484–1488. http://http://www.ncbi.nlm.nih.gov/pmc/articles/pmc1149544/. Accessed 23 Oct 2013

12.

Dogan N, Erdem A, Erman Z, Kizilkaya M (2004) The effects of bupivacaine and neostigmine on articular cartilage and synovium in the rabbit knee joint. J Int Med Res 32(5):513–519. doi:10.1177/0363546507304136 PubMedCrossRef

13.

Piper SL, Kim HT (2008) Comparison of ropivacaine and bupivacaine toxicity in human articular chondrocytes. J Bone Joint Surg Am 90(5):986–991. doi:10.1007/s00167-011-1420-5 PubMedCrossRef

14.

Yang C-Y, Cheng S-C, Shen C-L (1993) Effect of irrigation fluids on the articular cartilage: a scanning electron microscope study. Arthroscopy 9(4):425–430. doi:10.1097/00000542-200212000-00019 PubMedCrossRef

15.

Jurvelin J, Jurvelin J, Kiviranta I, Klauser R (1994) Effects of different irrigation liquids and times on articular cartilage: an experimental, biomechanical study. Arthroscopy 10(6):667–672. doi:10.1016/s0749-8063(05)80066-0 PubMedCrossRef

16.

Mcalinden A, Johnstone B, Kollar J, Kazmi N, Hering TM (2008) Expression of two novel alternatively spliced COL2A1 isoforms during chondrocyte differentiation. Matrix Biol 27(3):254–266. doi:10.1177/0363546507304719 PubMedCentralPubMedCrossRef

17.

Bigger JT Jr, Mandel WJ (1970) Effect of lidocaine on the electrophysiological properties of ventricular muscle and Purkinje fibers. J Clin Investig 49(1):63. doi:10.1002/jor.20507 PubMedCentralPubMedCrossRef

18.

Gold MS, Reichling DB, Hampl KF, Drasner K, Levine JD (1998) Lidocaine toxicity in primary afferent neurons from the rat. J Pharmacol Exp Ther 285(2):413–421. doi:10.1016/j.arthro.2009.03.019 PubMed

19.

Johnson ME, Saenz JA, Dasilva AD, Uhl CB, Gores GJ (2002) Effect of local anesthetic on neuronal cytoplasmic calcium and plasma membrane lysis (necrosis) in a cell culture model. Anesthesiology 97(6):1466–1476. doi:10.1002/art.1780380320 PubMedCrossRef

20.

Yamashita A, Matsumoto M, Matsumoto S, Itoh M, Kawai K, Sakabe T (2003) A comparison of the neurotoxic effects on the spinal cord of tetracaine, lidocaine, bupivacaine, and ropivacaine administered intrathecally in rabbits. Anesth Analg 97(2):512–519. doi:10.1016/j.matbio.2007.10.002 PubMedCrossRef

21.

Lo LK, Sciore P, Chung M, Liang S, Boorman RB, Thornton GM, Rattner JB, Muldrew K (2009) Local anesthetics induce chondrocyte death in bovine articular cartilage disks in a dose-and duration-dependent manner. Arthroscopy 25(7):707–715. doi:0.1007/s00167-010-1369-9 PubMedCrossRef

22.

Miyazaki T, Kobayashi S, Takeno K, Yayama T, Meir A, Baba H (2011) Lidocaine cytotoxicity to the bovine articular chondrocytes in vitro: changes in cell viability and proteoglycan metabolism. Knee Surg Sports Traumatol Arthrosc 19(7):1198–1205. doi:10.1177/0363546503262176 PubMedCrossRef

23.

Grouselle M, Tueux O, Dabadie P, Georgescaud D, Mazat J (1990) Effect of local anaesthetics on mitochondrial membrane potential in living cells. Biochem J 271(1):269. doi:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1149544/ PubMedCentralPubMed

24.

Aigner T, Stöß H, Weseloh G, Zeiler G, Von Der Mark K (1992) Activation of collagen type II expression in osteoarthritic and rheumatoid cartilage. Virchows Arch B Cell Pathol Incl Mol Pathol 62(1):337–345. doi:10.2214/ajr.05.1477 PubMedCrossRef

25.

Matyas JR, Adams ME, Huang D, Sandell LJ (1995) Discoordinate gene expression of aggrecan and type II collagen in experimental osteoarthritis. Arthritis Rheum 38(3):420–425. doi:10.1002/art.178027040 PubMedCrossRef

26.

Sandy JD, Adams ME, Billingham ME, Plaas A, Muir H (1984) In vivo and in vitro stimulation of chondrocyte biosynthetic activity in early experimental osteoarthritis. Arthritis Rheum 27(4):388–397. doi:10.1093/bja/aep035 PubMedCrossRef

27.

Kobayashi S, Meir A, Urban J (2008) Effect of cell density on the rate of glycosaminoglycan accumulation by disc and cartilage cells in vitro. J Orthop Res 26(4):493–503. doi:10.1016/s0749-8063(05)80317-2 PubMedCrossRef

Title: An evaluation of the delayed effect of intra-articular injections of lidocaine (2 %) on articular cartilage: an experimental study in rabbits
Authors: Hamidreza Yazdi
Bahahreh Tabatabaeian Nimavard
Mohammadali Shokrgozar
Mohammadmehdi Dehghan
Reza Jamei Moayedi
Mohammad Majidi
Tahmineh Mokhtari
Publication date: 01-12-2014
Publisher: Springer Paris
Published in: European Journal of Orthopaedic Surgery & Traumatology / Issue 8/2014
Print ISSN: 1633-8065
Electronic ISSN: 1432-1068
DOI: https://doi.org/10.1007/s00590-014-1437-9

At a glance: The STEP trials

Springer Medicine

An evaluation of the delayed effect of intra-articular injections of lidocaine (2 %) on articular cartilage: an experimental study in rabbits

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 8/2014

Reducing the risk of flexor pollicis longus tendon rupture after volar plate fixation for distal radius fractures: validation of the tendon irritation test

Diagnosing joint infections: synovial fluid differential is more sensitive than white blood cell count

Clinical outcome of autologous bone marrow aspirates concentrate (BMAC) injection in degenerative arthritis of the knee

Results of 30 reverse shoulder prostheses for revision of failed hemi- or total shoulder arthroplasty

Dominique G. Poitout and Henri Judet (Eds.): Mini-invasive surgery of the hip

Will gender-specific total knee arthroplasty be a better choice for women? A systematic review and meta-analysis