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Molecular Cancer
Published in: Molecular Cancer 1/2010

Open Access 01-12-2010 | Research

An essential role for Ran GTPase in epithelial ovarian cancer cell survival

Authors: Véronique Barrès, Véronique Ouellet, Julie Lafontaine, Patricia N Tonin, Diane M Provencher, Anne-Marie Mes-Masson

Published in: Molecular Cancer | Issue 1/2010

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Abstract

Background

We previously identified that Ran protein, a member of the Ras GTPase family, is highly expressed in high grade and high stage serous epithelial ovarian cancers, and that its overexpression is associated with a poor prognosis. Ran is known to contribute to both nucleocytoplasmic transport and cell cycle progression, but its role in ovarian cancer is not well defined.

Results

Using a lentivirus-based tetracycline-inducible shRNA approach, we show that downregulation of Ran expression in aggressive ovarian cancer cell lines affects cellular proliferation by inducing a caspase-3 associated apoptosis. Using a xenograft tumor assay, we demonstrate that depletion of Ran results in decreased tumorigenesis, and eventual tumor formation is associated with tumor cells that express Ran protein.

Conclusion

Our results suggest a role for Ran in ovarian cancer cell survival and tumorigenicity and suggest that this critical GTPase may be suitable as a therapeutic target.
Appendix
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Metadata
Title
An essential role for Ran GTPase in epithelial ovarian cancer cell survival
Authors
Véronique Barrès
Véronique Ouellet
Julie Lafontaine
Patricia N Tonin
Diane M Provencher
Anne-Marie Mes-Masson
Publication date
01-12-2010
Publisher
BioMed Central
Published in
Molecular Cancer / Issue 1/2010
Electronic ISSN: 1476-4598
DOI
https://doi.org/10.1186/1476-4598-9-272

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