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01-08-2018 | Original Paper

Amelioration of lactic acid sensations in sensitive skin by stimulating the barrier function and improving the ceramide profile

Authors: Hiroshi Nojiri, Koichi Ishida, Xueqiu Yao, Wei Liu, Genji Imokawa

Published in: Archives of Dermatological Research | Issue 6/2018

Abstract

We determined whether compensating ceramides in the stratum corneum (SC) may ameliorate the impaired barrier function and subsequently attenuate the enhanced skin sensitivity. Treatment for 4 weeks with the ceramide complex cream or the placebo cream significantly ameliorated the intensity of lactic acid sensations in 39 female subjects with sensitive skin, the degree of which was attenuated to a greater extent at 1 week by the ceramide complex cream compared with the placebo cream. The amelioration of skin sensations was accompanied by a significant increase in total ceramide content in the SC elicited by the ceramide complex cream that was significantly more effective than the placebo cream at 4 weeks. Consistently, TEWL and conductance values were significantly decreased or increased at 1 and 4 weeks, respectively, to a greater extent by the ceramide complex cream than by the placebo cream. TEWL levels were significantly correlated with the increased levels of SC total ceramide in the ceramide complex cream-treated skin but not in the placebo cream-treated skin. Thus, the amelioration of lactic acid sensations by topical application of a ceramide complex cream, provides a deep insight into the pathophysiology of sensitive skin as a reduced barrier function-dependent sub-clinical sensory response.

Arikawa J, Ishibashi M, Kawashima M, Takagi Y, Ichikawa Y, Imokawa G (2002) Decreased levels of sphingosine, a natural anti-microbial agent, may be associated with vulnerability of the stratum corneum from patients with atopic dermatitis to colonization by Staphylococcus aureus. J Invest Dermatol 119(2):433–439CrossRefPubMed

De Lacharrière O (2002) Peaux sensibles, peaux réactives. Cosmétologie et Dermatologie Esthétique 50-220-A-10

Di Nardo A, Wertz P, Giannetti A, Seidenari S (1998) Ceramide and cholesterol composition of the skin of patients with atopic dermatitis. Acta Derm Venereol 78(1):27–30CrossRefPubMed

Duan J, Sugawara T, Hirose M, Aida K, Sakai S, Fujii A, Hirata T (2012) Dietary sphingolipids improve skin barrier functions via the upregulation of ceramide synthases in the epidermis. Exp Dermatol 21(6):448–452CrossRefPubMed

Farage MA, Katsarou A, Maibach HI (2006) Sensory, clinical and physiological factors in sensitive skin: a review. Contact Dermatitis 55(1):1–14CrossRefPubMed

Forsch PJ, Kligman AM (1977) A method for appraising the stinging capacity of topically applied substances. J Soc Cosmet Chem 28(5):197–209

Funasaka Y, Bito T, Yamamoto M, Nishigori C, Ishihashi M, Nakamura T, Ishida K, Sato H, Imokawa G (2004) Clinical evaluation of “Curel UV Milk” and “Curel UV Cream” in subjects with low barrier function’s skin. Skin Res 31(1):62–74

Hata M, Tokura Y, Takigawa M, Tamura Y, Imokawa G (2002) Efficacy of using pseudoceramide-containg cream for the treatment of atopic dry skin in comparison with urea cream. Nishihihon J Dermatol 64(5):606–611CrossRef

Holleran WM, Feingold KR, Man M-Q, Gao WN, Lee JM, Elias PM (1991) Regulation of epidermal sphingolipid synthesis by permeability barrier function. J Lipid Res 32(7):1151–1158PubMed

10.

Holleran WM, Man MQ, Gao WN, Menon GK, Elias PM, Feingold KR (1991) Sphingolipids are required for mammalian epidermal barrier function. Inhibition of sphingolipid synthesis delays barrier recovery after acute perturbation. J Clin Invest 88(4):1338–1345CrossRefPubMedPubMedCentral

11.

Holleran WM, Takagi Y, Menon GK, Legler G, Feingold KR, Elias PM (1993) Processing of epidermal glucosylceramides is required for optimal mammalian cutaneous permeability barrier function. J Clin Invest 91(4):1656–1664CrossRefPubMedPubMedCentral

12.

Holleran WM, Ginns EI, Menon GK, Grundmann JU, Fartasch M, McKinney CE, Elias PM, Sidransky E (1994) Consequences of beta-glucocerebrosidase deficiency in epidermis. ultrastructure and permeability barrier alterations in Gaucher disease. J Clin Invest 93(4):1756–1764CrossRefPubMedPubMedCentral

13.

Holleran WM, Takagi Y, Menon GK, Jackson SM, Lee JM, Feingold KR, Elias PM (1994) Permeability barrier requirements regulate epidermal beta-glucocerebrosidase. J Lipid Res 35(5):905–912PubMed

14.

Imokawa G (1999) Skin moisturizers: development and clinical use ceramides. In: Loden M (ed) Dry skin and moisturizers. CRC Press, pp 269–299

15.

Imokawa G (2001) Ceramides as natural moisturizing factors and their efficacy in dry skin. In: Leyden JJ, Rawlings AV (eds) Skin moisturization. Marcel Dekker Inc., pp 267–302

16.

Imokawa G (2004) Surfactant-induced depletion of ceramides and other intercellular lipids: implication for the mechanism leading to dehydration of the stratum corneum. Exog Dermatol 3(2):81–98CrossRef

17.

Imokawa G, Kuno H, Kawai M (1991) Stratum corneum lipids serve as a bound-water modulator. J Invest Dermatol 96(6):845–851CrossRefPubMed

18.

Imokawa G, Yada Y, Higuchi K, Okuda M, Ohashi Y, Kawamata A (1994) Pseudo-acylceramide with linoleic acid produces selective recovery of diminished cutaneous barrier function in essential fatty acid deficient rats and has anvinhibitory effect on epidermal hyperplasia. J Clin Invest 94(1):89–96CrossRefPubMedPubMedCentral

19.

Imokawa G, Abe A, Jin K, Higaki Y, Kawashima M, Hidano A (1991) Decreased level of ceramides in stratum corneum of atopic dermatitis: an etiologic factor in atopic dry skin? J Invest Dermatol 96(4):523–526CrossRefPubMed

20.

Ishibashi M, Arikawa J, Okamoto R, Kawashima M, Takagi Y, Oguchi K, Imokawa G (2003) The abnormal expression of the novel epidermal enzyme, glucosylceramide deacylase and the accumulation of its enzymatic reaction product, glucosylsphingosine in the skin of patients with atopic dermatitis. Lab Invest 88(3):397–408CrossRef

21.

Ishikawa J, Narita H, Kondo N, Hotta M, Takagi Y, Masukawa Y, Kitahara T, Takema Y, Koyano S, Yamazaki S, Hatamochi A (2010) Changes in the ceramide profile of atopic dermatitis patients. J Invest Dermatol 130(10):2511–2514CrossRefPubMed

22.

Ishikawa J, Shimotoyodome Y, Chen S, Ohkubo K, Takagi Y, Fujimura T, Kitahara T, Takema Y (2012) Eucalyptus increases ceramide levels in keratinocytes and improves stratum corneum function. Inter J Cos Sci 34(1):17–22CrossRef

23.

Janssens M, van Smeden J, Gooris GS, Bras W, Portale G, Caspers PJ, Vreeken RJ, Hankemeier T, Kezic S, Wolterbeek R, Lavrijsen AP, Bouwstra JA (2012) Increase in short-chain ceramides correlates with an altered lipid organization and decreased barrier function in atopic eczema patients. J Lipid Res 53(10):2755–2766CrossRefPubMedPubMedCentral

24.

Janssens M, van Smeden J, Gooris GS, Bras W, Portale G, Caspers PJ, Vreeken RJ, Kezic S, Lavrijsen AP, Bouwstra JA (2011) Lamellar lipid organization and ceramide composition in the stratum corneum of patients with atopic eczema. J Invest Dermatol 131(12):2136–2138CrossRefPubMed

25.

Jungersted JM, Scheer H, Mempel M, Baurecht H, Cifuentes L, Høgh JK, Hellgren LI, Jemec GB, Agner T, Weidinger S (2010) Stratum corneum lipids, skin barrier function and filaggrin mutations in patients with atopic eczema. Allergy 65(7):911–918CrossRefPubMed

26.

Kawasaki H, Nagao K, Kubo A, Hata T, Shimizu A, Mizuno H, Yamada T, Amagai M (2012) Altered stratum corneum barrier and enhanced percutaneous immune responses in filaggrin null mice. J Allergy Clin Immunol 129(6):1538–1546CrossRefPubMed

27.

Koyano S, Hatamochi A, Yamazaki S, Ishikawa J, Kitahara T, Narita H, Kondo N, Masukawa Y (2010) Psoriasis patients have abnormal ceramide profile in straum corneum. Nishihihon J Dermatol 72(5):494–499CrossRef

28.

Luger TA (2002) Neuromediators: a crucial component of the skin immune system. J Dermatol Sci 30(2):87–93CrossRefPubMed

29.

Masukawa Y, Narita H, Shimizu E, Kondo N, Sugai Y, Oba T, Homma R, Ishikawa J, Takagi Y, Kitahara T, Takema Y, Kita K (2008) Characterization of overall ceramide species in human stratum corneum. J Lipid Res 49(7):1466–1476CrossRefPubMed

30.

Masukawa Y, Narita H, Sato H, Naoe A, Kondo N, Sugai Y, Oba T, Homma R, Ishikawa J, Takagi Y, Kitahara T (2009) Comprehensive quantification of ceramide species in human stratum corneum. J Lipid Res 50(8):1708–1719CrossRefPubMedPubMedCentral

31.

Matsuki H (2006) Significance of barrier repair in non-lesinal dry skin of atopic dermatitis: Efficacy of a topically applied ceramide cream. Nishihihon J Dermatol 64:606–611

32.

Matsuki H, Kiyokane K, Matsuki T, Sato S, Imokawa G (2004) Re-evaluation of the importance of barrier dysfunction in the nonlesional dry skin of atopic dermatitis through the use of two barrier creams. Exog Dermatol 3(6):293–302CrossRef

33.

Misery L (1997) Skin, immunity and the nervous system. Br J Dermatol 137(6):843–850CrossRefPubMed

34.

Misery L, Myon E, Martin N, Verriere F, Nocera T, Taieb C (2005) Sensitive skin in France: an epidemiological approach. Ann Dermatol Venereol 132(5):425–429CrossRefPubMed

35.

Mizutani J, Takahashi M, Shimizu M, Kariya N, Sato H, Imokawa G (2001) Usage of pseudoceramide cream in atopic dry skin in comparison with 10% urea cream. Nishihihon J Dermatol 63(4):457–461CrossRef

36.

Nakajima K, Terao M, Takaishi M, Kataoka S, Goto-Inoue N, Setou M, Horie K, Sakamoto F, Ito M, Azukizawa H, Kitaba S, Murota H, Itami S, Katayama I, Takeda J, Sano S (2013) Barrier abnormality due to ceramide deficiency leads to psoriasiform inflammation in a mouse model. J Invest Dermatol 133(11):2555–2565CrossRefPubMed

37.

Nakamura T, Satoh H, Imokawa G, Miyachi Y (2000) Clinical test of skin care products on atopic dry skin. Skin Res 42(2):264–269

38.

Ohta M, Hikima R, Ogawa T (2000) Physiological characteristics of sensitive skin classified by stinging test. J Cosmet Sci Soc Jpn 24(3):163–167

39.

Okamoto R, Arikawa J, Ishibashi M, Kawashima M, Takagi Y, Imokawa G (2003) Sphingosylphosphorylcholine is upregulated in the stratum corneum of patients with atopic dermatitis. J Lipid Res 44(1):93–102CrossRefPubMed

40.

Palmer CN, Irvine AD, Terron-Kwiatkowski A, Zhao Y, Liao H, Lee SP, Goudie DR, Sandilands A, Campbell LE, Smith FJ, O’Regan GM, Watson RM, Cecil JE, Bale SJ, Compton JG, DiGiovanna JJ, Fleckman P, Lewis-Jones S, Arseculeratne G, Sergeant A, Munro CS, El Houate B, McElreavey K, Halkjaer LB, Bisgaard H, Mukhopadhyay S, McLean WH (2006) Common loss-of-function variants of the epidermal barrier protein filaggrin are a major predisposing factor for atopic dermatitis. Nat Genet 38(4):441–446CrossRefPubMed

41.

Primavera G, Berardesca E (2005) Sensitive skin: mechanisms and diagnosis. Int J Cosmet Sci 27(1):1–10CrossRefPubMed

42.

Sandilands A, O’Regan GM, Liao H, Zhao Y, Terron-Kwiatkowski A, Watson RM, Cassidy AJ, Goudie DR, Smith FJ, McLean WH, Irvine AD (2006) Prevalent and rare mutations in the gene encoding filaggrin cause ichthyosis vulgaris and predispose individuals to atopic dermatitis. J Invest Dermatol 126(8):1770–1775CrossRefPubMed

43.

Sandilands A, Smith FJ, Irvine AD, McLean WH (2007) Filaggrin’s fuller figure: a glimpse into the genetic architecture of atopic dermatitis. J Invest Dermatol 127(6):1282–1284CrossRefPubMed

44.

Ständer S, Schneider SW, Weishaupt C, Luger TA, Misery L (2009) Putative neuronal mechanisms of sensitive skin. Exp Dermatol 18(5):417–423CrossRefPubMed

45.

Sugiura A, Nomura T, Mizuno A, Imokawa G (2014) Reevaluation of the non-lesional dry skin in atopic dermatitis by acute barrier disruption: An abnormal permeability barrier homeostasis with defective processing to generate ceramide. Arch Dermatol Res 306(5):427–440CrossRefPubMed

46.

Takagi Y, Nakagawa H, Higuchi K, Imokawa G (2005) Characterization of surfactant-induced skin damage through barrier recovery induced by pseudoacylceramides. Dermatology 211(2):128–134CrossRefPubMed

47.

Takashima I, Hasebe K, Okuda M, Imokawa G (2002) Efficacy of pseudoceramide-containg detergent formulation for treatment of atopic dermatitis and asteatotic eczema. Nishihihon J Dermatol 64(5):612–620CrossRef

48.

Umeda Y, Mizutani H, Imokawa G, Shimizu M (1997) Topical ceramide corrected epidermal cell hyperproliferation and stratum corneum dysmaturation in atopic eczema. In: Johannes R, Heidrun B, Dieter V (eds) New trends in allergy IV. Springer, Berlin, pp 237–239CrossRef

49.

van Drongelen V, Alloul-Ramdhani M, Danso MO, Mieremet A, Mulder A, van Smeden J, Bouwstra JA, El Ghalbzouri A (2013) Knock-down of filaggrin does not affect lipid organization and composition in stratum corneum of reconstructed human skin equivalents. Exp Dermatol 22(12):807–812CrossRefPubMed

50.

Yanyu W, Wang X, Zhao Y, Tan Y, Chen D, Chen Y, Ye M (2003) Correlation between stinging, TEWL and capacitance. Skin Res Tech 9(2):90–93CrossRef

51.

Yokota T, Matsumoto M, Sakamati T (2003) Classification of sensitive skin and development of a treatment system appropriate for each group. IFSCC Mag 6(4):303–307

52.

Yamanaka M, Tshibashi O, Takahashi A, Sato H, Imokawa G (2001) Clinical evaluation of Curel medicated cream in patients with atopic dermatitis. Skin Res 43(4–5):278–285

Title: Amelioration of lactic acid sensations in sensitive skin by stimulating the barrier function and improving the ceramide profile
Authors: Hiroshi Nojiri
Koichi Ishida
Xueqiu Yao
Wei Liu
Genji Imokawa
Publication date: 01-08-2018
Publisher: Springer Berlin Heidelberg
Published in: Archives of Dermatological Research / Issue 6/2018
Print ISSN: 0340-3696
Electronic ISSN: 1432-069X
DOI: https://doi.org/10.1007/s00403-018-1833-9

At a glance: The STEP trials

Springer Medicine

Amelioration of lactic acid sensations in sensitive skin by stimulating the barrier function and improving the ceramide profile

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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