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01-06-2007 | Original Paper

Alteration of serum and tumoral neural cell adhesion molecule (NCAM) isoforms in patients with brain tumors

Authors: Laura Todaro, Silvia Christiansen, Mirta Varela, Paola Campodónico, M. Guadalupe Pallotta, José Lastiri, Eugenia Sacerdote de Lustig, Elisa Bal de Kier Joffé, Lydia Puricelli

Published in: Journal of Neuro-Oncology | Issue 2/2007

Abstract

The neural cell adhesion molecule (NCAM) is involved in the intercellular junctions of neurons and glial cells. We investigated its relevance as a biomarker in gliomas which main characteristic is their high invasiveness.

We studied by Western blot the pattern of serum NCAM bands in patients with gliomas (n = 34), with brain metastasis of different primary cancers (n = 27) and with benign brain tumors (n = 22)] compared with healthy controls (n = 69). For densitometric analysis NCAM bands ≥130 kDa (HMW) and <130 kDa (LMW) were clustered. We observed that glioma patients presented higher NCAM HMW and lower NCAM LMW levels than control subjects (P < 0.01). A similar pattern was found in patients with brain metastasis or brain benign tumors, suggesting that the pattern of serum NCAM bands would be useful to detect brain tumor pathology. On the other hand, serum NCAM expression was not associated with the main clinicopathological features of gliomas, including overall survival. Interestingly, we found that 9/12 patients with glioma showed a significant decrease in NCAM HMW/LMW ratio between 1–3 months after successful tumor removal. Thus, serum NCAM could be a useful marker for monitoring treatment.

NCAM expression was also analyzed at tissular level in 59 glioma sections from paraffined tumors. We observed that NCAM immunostaining was inversely correlated with the histological grade of malignancy, remaining this association in a multivariate analysis. Besides, loss of NCAM staining was significantly associated with bad prognosis in an univariate analysis.

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Title: Alteration of serum and tumoral neural cell adhesion molecule (NCAM) isoforms in patients with brain tumors
Authors: Laura Todaro
Silvia Christiansen
Mirta Varela
Paola Campodónico
M. Guadalupe Pallotta
José Lastiri
Eugenia Sacerdote de Lustig
Elisa Bal de Kier Joffé
Lydia Puricelli
Publication date: 01-06-2007
Publisher: Kluwer Academic Publishers-Plenum Publishers
Published in: Journal of Neuro-Oncology / Issue 2/2007
Print ISSN: 0167-594X
Electronic ISSN: 1573-7373
DOI: https://doi.org/10.1007/s11060-006-9312-0

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Alteration of serum and tumoral neural cell adhesion molecule (NCAM) isoforms in patients with brain tumors

Abstract

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Abstract

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