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01-10-2017 | Original Article

Airway reconstruction using decellularized tracheal allografts in a porcine model

Authors: Michinobu Ohno, Yasushi Fuchimoto, Huai-Che Hsu, Masataka Higuchi, Makoto Komura, Tetsuji Yamaoka, Akihiro Umezawa, Shin Enosawa, Tatsuo Kuroda

Published in: Pediatric Surgery International | Issue 10/2017

Abstract

Purpose

Tracheal cartilage reconstruction is an essential approach for the treatment of tracheal congenital abnormalities or injury. Here, we evaluated the use of allogeneic decellularized tracheas as novel support scaffolds.

Methods

Six weaned pigs (4-week-old domestic males) were transplanted with allogeneic tracheal graft patches (three decellularized and three fresh tracheal scaffolds) onto artificial defects (approximately 15 × 15 mm). After 11 weeks, the tracheas were evaluated by bronchoscopy and histological studies.

Results

No pigs displayed airway symptoms during the observation period. Tracheal lumen restored by fresh graft patches showed more advanced narrowing than that treated with decellularized grafts by bronchoscopy. Histologically, fresh grafts induced typical cellular rejection; this was decreased with decellularized grafts. In addition, immunohistochemistry demonstrated regenerating foci of recipient cartilage along the adjacent surface of decellularized tracheal grafts.

Conclusion

Decellularized allogeneic tracheal scaffolds could be effective materials for restoring impaired trachea.

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Title: Airway reconstruction using decellularized tracheal allografts in a porcine model
Authors: Michinobu Ohno
Yasushi Fuchimoto
Huai-Che Hsu
Masataka Higuchi
Makoto Komura
Tetsuji Yamaoka
Akihiro Umezawa
Shin Enosawa
Tatsuo Kuroda
Publication date: 01-10-2017
Publisher: Springer Berlin Heidelberg
Published in: Pediatric Surgery International / Issue 10/2017
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-017-4138-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Airway reconstruction using decellularized tracheal allografts in a porcine model

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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