Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2017

Open Access 01-12-2017 | Research article

Agrimonia pilosa Ledeb. aqueous extract improves impaired glucose tolerance in high-fat diet-fed rats by decreasing the inflammatory response

Authors: Hwan Hee Jang, Song Yee Nam, Mi Ju Kim, Jung Bong Kim, Jeong Sook Choi, Haeng Ran Kim, Young Min Lee

Published in: BMC Complementary Medicine and Therapies | Issue 1/2017

Login to get access

Abstract

Background

Agrimonia pilosa Ledeb. is a medicinal plant with physiological activities such as anti-cancer, antioxidant, anti-inflammatory activities and in vitro anti-diabetic activity. However, the effects of aqueous extracts from A. pilosa on insulin-resistant rats have not yet been examined. We investigated the effects of aqueous extract from A. pilosa on impaired glucose metabolism induced by a high-fat diet in rats.

Methods

Male Sprague-Dawley rats were assigned to the following groups: normal-fat diet (NF, n = 9); high-fat diet (HF, n = 9); high-fat diet with 0.1% A. pilosa aqueous extract (HFA, n = 10). Experimental diets were administered for 16 weeks. At the end of the treatment, liver and fat tissues were isolated, and serum was collected for biochemical analysis.

Results

The HF group rats had a significantly higher liver weight than the NF group rats did, and increased hepatic lipid accumulation (p < 0.05); however, supplementation with A. pilosa decreased liver weight. Blood glucose levels in the HFA group were lower than levels measured in the HF group 30, 60, and 120 min after glucose administration (p < 0.05). In addition, dietary A. pilosa supplementation decreased tumor necrosis factor α and interleukin 6 levels, while increasing serum adiponectin concentrations (p < 0.05 vs. the HF group). These effects were accompanied by reduced hepatic and adipose tissue expression of inflammation-related genes such as Tnf and Il1b (p < 0.05).

Conclusions

Our findings indicate that A. pilosa aqueous extract can ameliorate insulin resistance in high-fat diet-fed rats by decreasing the inflammatory response.
Appendix
Available only for authorised users
Literature
1.
2.
Kadowaki T, Hara K, Yamauchi T, Terauchi Y, Tobe K, Nagai R. Molecular mechanism of insulin resistance and obesity. Exp Biol Med (Maywood). 2003;228:1111–7.CrossRef
3.
Goldstein BJ. Insulin resistance as the core defect in type 2 diabetes mellitus. Am J Cardiol. 2002;90:3g–10g.CrossRefPubMed
4.
Reaven GM. The insulin resistance syndrome: definition and dietary approaches to treatment. Annu Rev Nutr. 2005;25:391–406.CrossRefPubMed
5.
Utzschneider KM, Kahn SE. Review: the role of insulin resistance in nonalcoholic fatty liver disease. J Clin Endocrinol Metab. 2006;91:4753–61.CrossRefPubMed
6.
Mulhall BP, Ong JP, Younossi ZM. Non-alcoholic fatty liver disease: an overview. J Gastroenterol Hepatol. 2002;17:1136–43.CrossRefPubMed
7.
8.
9.
Kadowaki T, Yamauchi T, Kubota N, Hara K, Ueki K, Tobe K. Adiponectin and adiponectin receptors in insulin resistance, diabetes, and the metabolic syndrome. J Clin Investig. 2006;116:1784.CrossRefPubMedPubMedCentral
10.
Miyamoto K, Kishi N, Koshiura R. Antitumor effect of agrimoniin, a tannin of Agrimonia pilosa Ledeb., on transplantable rodent tumors. Jpn J Pharmacol. 1987;43:187.CrossRefPubMed
11.
He C, Ji X, Pan Y, Wang H, Wang K, Liang M, Yang L. Antioxidant activity of alcoholic extract of Agrimonia pilosa Ledeb. Med Chem Res. 2010;19:448–61.CrossRef
12.
Jung M, Park M. Acetylcholinesterase inhibition by flavonoids from Agrimonia pilosa. Molecules. 2007;12:2130–9.CrossRefPubMed
13.
Taira J, Nanbu H, Ueda K. Nitric oxide-scavenging compounds in Agrimonia pilosa Ledeb on LPS-induced RAW264.7 macrophages. Food Chem. 2009;115:1221–7.CrossRef
14.
Jung CH, Kim JH, Park SJ, Kweon DH, Kim SH, Ko SG. Inhibitory effect of Agrimonia pilosa Ledeb. On inflammation by suppression of iNOS and ROS production. Immunol Investig. 2010;39:159–70.CrossRef
15.
Kim SM, Lee YM, Kim M-J, Nam S-Y, Kim S-H, Jang H-H. Effects of Agrimonia Pilosa Ledeb. Water extract on α-Glucosidase inhibition and glucose uptake in C2C12 skeletal muscle cells. Korean J Food & Nutr. 2013;26:806–13.CrossRef
16.
Jang H-H, Park M-Y, Kim H-W, Lee Y-M, Hwang K-A, Park J-H, Park D-S, Kwon O. Black rice (Oryza Sativa L.) extract attenuates hepatic steatosis in C57BL/6 J mice fed a high-fat diet via fatty acid oxidation. Nutri & Metab. 2012;9:27.CrossRef
17.
Gregor MF, Hotamisligil GS. Inflammatory mechanisms in obesity. Annu Rev Immunol. 2011;29:415–45.CrossRefPubMed
18.
Hotamisligil GS, Arner P, Caro JF, Atkinson RL, Spiegelman BM. Increased adipose tissue expression of tumor necrosis factor alpha in human obesity and insulin resistance. J Clin Invest. 1995;95:2409.CrossRefPubMedPubMedCentral
19.
Weisberg SP, McCann D, Desai M, Rosenbaum M, Leibel RL, Ferrante AW. Obesity is associated with macrophage accumulation in adipose tissue. J Clin Invest. 2003;112:1796–808.CrossRefPubMedPubMedCentral
20.
Cai D, Yuan M, Frantz DF, Melendez PA, Hansen L, Lee J, Shoelson SE. Local and systemic insulin resistance resulting from hepatic activation of IKK-β and NF-κB. Nat Med. 2005;11:183–90.CrossRefPubMedPubMedCentral
21.
22.
Kim J-Y, van de Wall E, Laplante M, Azzara A, Trujillo ME, Hofmann SM, Schraw T, Durand JL, Li H, Li G. Obesity-associated improvements in metabolic profile through expansion of adipose tissue. J Clin Invest. 2007;117:2621–37.CrossRefPubMedPubMedCentral
23.
Tilg H, Hotamisligil GS. Nonalcoholic fatty liver disease: cytokine-adipokine interplay and regulation of insulin resistance. Gastroenterol. 2006;131:934–45.CrossRef
24.
Park J, Rho HK, Kim KH, Choe SS, Lee YS, Kim JB. Overexpression of glucose-6-phosphate dehydrogenase is associated with lipid dysregulation and insulin resistance in obesity. Mol Cell Biol. 2005;25:5146–57.CrossRefPubMedPubMedCentral
25.
Juhan-Vague I, Alessi MC, Mavri A, Morange P. Plasminogen activator inhibitor-1, inflammation, obesity, insulin resistance and vascular risk. J Thromb Haemostasis. 2003;1:1575–9.CrossRef
26.
Ueki K, Kondo T, Kahn CR. Suppressor of cytokine signalling 1 (SOCS-1) and SOCS-3 cause insulin resistance through inhibition of tyrosine phosphorylation of insulin receptor substrate proteins by discrete mechanisms. Mol Cell Biol. 2004;24:5434–46.CrossRefPubMedPubMedCentral
27.
Collino M, Aragno M, Castiglia S, Miglio G, Tomasinelli C, Boccuzzi G, Thiemermann C, Fantozzi R. Pioglitazone improves lipid and insulin levels in overweight rats on a high cholesterol and fructose diet by decreasing hepatic inflammation. Brit J Pharmacol. 2010;160:1892–902.CrossRef
28.
Xu X, Qi X, Wang W, Chen G. Separation and determination of flavonoids in Agrimonia pilosa Ledeb. By capillary electrophoresis with electrochemical detection. J Sep Sci. 2005;28:647–52.CrossRefPubMed
29.
Miyamoto K, Koshiura R, Ikeya Y, Taguchi H. Isolation of agrimoniin, an antitumor constituent, from the roots of Agrimonia pilosa Ledeb. Chem Pharm Bull. 1985;33:3977–81.CrossRef
30.
Lee YM, Kim JB, Bae JH, Lee JS, Kim PS, Jang HH, Kim HR. Estrogen-like activity of aqueous extract from Agrimonia pilosa Ledeb. In MCF-7 cells. BMC Complement Altern Med. 2012;12:260.CrossRefPubMedPubMedCentral
31.
Metadata
Title
Agrimonia pilosa Ledeb. aqueous extract improves impaired glucose tolerance in high-fat diet-fed rats by decreasing the inflammatory response
Authors
Hwan Hee Jang
Song Yee Nam
Mi Ju Kim
Jung Bong Kim
Jeong Sook Choi
Haeng Ran Kim
Young Min Lee
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2017
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-017-1949-z

Other articles of this Issue 1/2017

BMC Complementary Medicine and Therapies 1/2017 Go to the issue

Research article

Traditional healer treatment of HIV persists in the era of ART: a mixed methods study from rural South Africa

Research article

XuefuZhuyu decoction protected cardiomyocytes against hypoxia/reoxygenation injury by inhibiting autophagy

Research article

In vivo screening and evaluation of four herbs against MRSA infections

Research article

Neuroprotective effect of salvianolate lyophilized injection against cerebral ischemia in type 1 diabetic rats

Research article

Profile of bioactive compounds in Nymphaea alba L. leaves growing in Egypt: hepatoprotective, antioxidant and anti-inflammatory activity

Research article

Inhibition of human cytomegalovirus immediate-early gene expression and replication by the ethyl acetate (EtOAc) fraction of Elaeocarpus sylvestris in vitro