Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Lasers in Medical Science
Published in: Lasers in Medical Science 7/2016

01-09-2016 | Original Article

Adjunctive effect of antimicrobial photodynamic therapy in induced periodontal disease. Animal study with histomorphometrical, immunohistochemical, and cytokine evaluation

Authors: Paula Gabriela Faciola Pessôa de Oliveira, Adriana Maria Mariano Silveira e Souza, Arthur Belém Novaes Jr, Mário Taba Jr, Michel Reis Messora, Daniela Bazan Palioto, Márcio Fernando Moraes Grisi, Antônio Cláudio Tedesco, Sérgio Luis Scombatti de Souza

Published in: Lasers in Medical Science | Issue 7/2016

Login to get access

Abstract

Scaling and root planing (SRP) may not always be effective in preventing periodontal disease (PD) progression. The aim of this study was to evaluate the adjunctive effect of antimicrobial photodynamic therapy (aPDT) to SRP on induced PD in rats, analyzing histomorphometrical, immunohistochemical, and immunoenzymatic parameters. Ligatures were placed around the first mandibular molars and second maxillary molars of 60 rats to induce PD. After 14 days, they were removed and the animals were divided into six groups, with nine animals each: G1 = no treatment, G2 = SRP, G3 = light-emitting diode (LED), G4 = SRP + aPDT, G5 = aPDT, and G6 = erythrosine. The animals were euthanized after 3, 7, and 15 days. There were also two control groups (n = 3): without PD (WPD) induction and with maximum PD (PD+). In the histomorphometrical analysis of linear bone loss, G4 showed a statistically significant difference from the other experimental groups after 3 and 15 days. The tartrate-resistant acid phosphatase (TRAP)-positive cell counting was significantly lower in G4 when compared to G2 and PD+ after 3 days. Immunoenzymatic assay shows the values of the ratio (RANKL/OPG × 100). The lowest value is from the WPD group, and the group that received the SRP + aPDT treatment tended to approach this value over time. After 3 days, statistically significant differences were observed between G4 and all other experimental groups, as well as versus PD+ (one-way ANOVA + Tukey’s post hoc test were performed, p < 0.05). It was concluded that the adjunctive use of aPDT in combination with SRP showed the best therapeutic results in the treatment of periodontal disease in rats.
Literature
1.
2.
Sallum AW, Martins AG, Sallum EA (2004) A doença periodontal e o surgimento de um novo paradigma. In: Periodontia Médica: Uma abordagem integrada. Editora Senac São Paulo 21-39
3.
Trouvin AP, Goeb V (2010) Receptor activator of nuclear factor-kB ligand and osteoprotegerin: maintaining the balance to prevent bone loss. Clin Interv Aging 19:345–354
4.
Belibasakis GN, Bostanci N (2012) The RANKL-OPG system in clinical periodontology. J Clin Periodontol 39:239–248CrossRefPubMed
5.
Aoki A, Sasaki KM, Watanabe H, Ishikawa I (2004) Lasers in nonsurgical periodontal therapy. Periodontol 2000(36):59–97CrossRef
6.
Kaldahl WB, Kalkwarf KL, Patil KD (1993) A review of longitudinal studies that compared periodontal therapies. J Periodontol 64:243–253CrossRefPubMed
7.
Kömerik N, Nakanishi H, MacRobert AJ, Henderson B, Speight P, Wilson M (2003) In vivo killing of Porphyromonas gingivalis by toluidine blue mediated photosensitization in an animal model. Antimicrob Agents Chemother 47:932–940CrossRefPubMedPubMedCentral
8.
Wilson M, Dohson J, Sarkar S (1993) Sensitization of periodontopathogenic bacteria to killing by light from a low-power laser. Oral Microbiol Immunol 8:182–187CrossRefPubMed
9.
10.
Zanin ICJ, Goncalves RB, Junior AB, Hope CK, Pratten J (2005) Susceptibility of Streptococcus mutans biofilms to photodynamic therapy: an in vitro study. J Antimicrob Chemother 56:324–330CrossRefPubMed
11.
Soares BM, Silva DL, Sousa GR, Amorim JCF, Resende MA, Pinotti M, Cisalpino PS (2009) In vitro photodynamic inactivation of Candida spp. growth and adhesion to buccal epithelial cells. Photochem Photobiol B Biol 94:65–70CrossRef
12.
Allaker RP, Douglas CWI (2009) Novel anti-microbial therapies for dental plaque related-diseases. Int J Antimicrob Agents 33:8–13CrossRefPubMed
13.
Wood S, Metcalf D, Devine D, Robinson C (2006) Erythrosine is a potential photosensitizer for the photodynamic therapy of oral plaque biofilms. J Antimicrob Chemother 57:680–684CrossRefPubMed
14.
Paulino TP, Ribeiro KF, Thedei G Jr, Tedesco A, Ciancaglini P (2005) Use of hand held photopolymerizer to photoinactivate Streptococcus mutans. Arch Oral Biol 50:353–359CrossRefPubMed
15.
Goulart RD, Bolean M, Paulino TD, Thedei G, Souza SL, Tedesco AC, Ciancaglini P (2010) Photodynamic therapy in planktonic and biofilm cultures of Aggregatibacter actinomycetemcomitans. Photomed Laser Surg 28:53–60CrossRef
16.
Goulart R, Thedei G, Souza S, Tedesco A, Ciancaglini P (2010) Comparative study of methylene blue and erythrosine dyes employed in photodynamic therapy for inactivation of planktonic and biofilm-cultivated Aggregatibacter actinomycetemcomitans. Photomed Laser Surg 28:85–90CrossRef
17.
Costa AC, Campos Rasteiro VM, da Silva Hashimoto ES, Araújo CF, Pereira CA, Junqueira JC, Jorge AO (2012) Effect of erythrosine- and LED-mediated photodynamic therapy on buccal candidiasis infection of immunosuppressed mice and Candida albicans adherence to buccal epithelial cells. Oral Surg Oral Med Oral Pathol Oral Radiol 114:67–74CrossRefPubMed
18.
Yankell SL, Loux JJ (1977) Acute toxicity testing of erythrosine and sodium fluorescein in mice and rats. J Periodontol 48:228–31CrossRefPubMed
19.
Rovin S, Costich ER, Gordon HA (1966) The influence of bacteria and irritation in the initiation of periodontal disease in germfree and conventional rats. J Periodontal Res 3:193–204CrossRef
20.
Oz HS, Puleo DA (2011) Animal models for periodontal disease. J Biomed Biotechnol 2011:1–8CrossRef
21.
22.
Berkstein S, Reiff RL, McKinney JF, Killoy WJ (1987) Supragingival root surface removal during maintenance procedures utilizing an air-powder abrasive system or hand scaling. An in vitro study. J Periodontol 58:327–330CrossRefPubMed
23.
Wasserman B, Hirschfeld I (1998) The relationship of initial clinical parameters to the long-term response in 112 cases of periodontal disease. J Clin Periodontol 15:38–42CrossRef
24.
Slots J, Rams TE (1990) Antibiotics in periodontal therapy: advantages and disadvantages. J Clin Periodontol 17:479–493CrossRefPubMed
25.
Taubman MA, Valverde P, Han X, Kawai T (2005) Immune response: the key to bone resorption in periodontal disease. J Periodontol 76:2033–2041CrossRefPubMed
26.
de Oliveira RR, Schwartz-Filho HO, Novaes AB Jr, Taba M Jr (2007) Antimicrobial photodynamic therapy in the non-surgical treatment of aggressive periodontitis: a preliminary randomized controlled clinical study. J Periodontol 78:965–973CrossRefPubMed
27.
Polansky R, Haas M, Heschl A, Wimmer G (2009) Clinical effectiveness of photodynamic therapy in the treatment of periodontitis. J Clin Periodontol 36:575–580CrossRefPubMed
28.
Almeida JM, Theodoro LH, Bosco AF, Nagata MJ, Oshiiwa M, Garcia VG (2007) Influence of photodynamic therapy on the development of ligature-induced periodontitis in rats. J Periodontol 78:566–575CrossRefPubMed
29.
Almeida JM, Theodoro LH, Bosco AF, Nagata MJH, Bonfante S, Garcia VG (2008) Treatment of experimental periodontal disease by photodynamic therapy in rats with diabetes. J Periodontol 79:2156–2165CrossRefPubMed
30.
Braun A, Dehn C, Kraus F, Jepsen S (2008) Short-term clinical effects of adunctive antimicrobial photodynamic therapy in periodontal treatment: a randomized clinical trial. J Clin Periodontol 35:877–884CrossRefPubMed
31.
Andersen R, Loeble N, Hammond D (2007) Treatment of periodontal disease by photodisinfection compared to scaling and root planing. J Clin Dent 18:34–38PubMed
32.
Garcia VG, Longo M, Gualberto Júnior EC, Bosco AF, Nagata MJ, Ervolino E, Theodoro LH (2014) Effect of the concentration of phenothiazine photosensitizers in antimicrobial photodynamic therapy on bone loss and the immune inflammatory response of induced periodontitis in rats. J Periodontal Res 5:584–594CrossRef
33.
Kajiya M, Giro G, Taubman MA, Han X, Mayer MP, Kawai T (2010) Role of periodontal pathogenic bacteria in RANKL-mediated bone destruction in periodontal disease. J Oral Microbiol 8:2. doi:10.​3402/​jom.​v2i0.​5532
34.
Theoleyre S, Wittrant Y, Tat SK, Fortun Y, Redini F, Heymann D (2004) The molecular triad OPG/RANK/RANKL: involvement in the orchestration of pathophysiological bone remodeling. Cytokine Growth Factor Rev 15:457–475CrossRefPubMed
35.
36.
Wara-aswapati N, Surarit R, Chayasadom A, Boch JA, Pitiphat W (2007) RANKL up regulation associated with periodontitis and Porphyromonas gingivalis. J Periodontol 78:1062–1069CrossRefPubMed
37.
Bostanci N, Ilgenli T, Emingil G, Afacan B, Han B, Töz H, Atilla G, Hughes FJ, Belibasakis GN (2007) Gingival crevicular fluid levels of RANKL and OPG in periodontal diseases: implications of their relative ratio. J Clin Periodontol 34:370–376CrossRefPubMed
38.
Lu HK, Chen YL, Chang HC, Li CL, Kuo MY (2006) Identification of the osteoprotegerin/receptor activator of nuclear factor-kappa B ligand system in gingival crevicular fluid and tissue of patients with chronic periodontitis. J Periodontal Res 41:354–360CrossRefPubMed
39.
Taubman MA, Kawai T, Han X (2007) The new concept of periodontal disease pathogenesis requires new and novel therapeutic strategies. J Clin Periodontol 34:367–369CrossRefPubMed
40.
Metcalf D, Robinson C, Devine D, Wood S (2006) Enhancement of erythrosine-mediated photodynamic therapy of Streptococcus mutans biofilms by light fractionation. J Antimicrob Chemother 58:190–192CrossRefPubMed
41.
Almeida JM, Theodoro LH, Bosco AF, Nagata MJ, Oshiiwa M, Garcia VG (2008) In vivo effect of photodynamic therapy on periodontal bone loss in dental furcations. J Periodontol 79:1081–1088CrossRefPubMed
42.
Christodoulides N, Nikolidakis D, Chondros P, Becker J, Schwarz F, Rössler R, Sculean A (2008) Photodynamic therapy as an adjunct to non-surgical periodontal treatment: a randomized, controlled clinical trial. J Periodontol 79:1638–1644CrossRefPubMed
43.
Chondros P, Nikolidakis D, Christodoulides N, Rössler R, Gutknecht N, Sculean A (2009) Photodynamic therapy as adjunct to non-surgical periodontal treatment in patients on periodontal maintenance: a randomized controlled clinical trial. Lasers Med Sci 24:681–688CrossRefPubMed
44.
de Oliveira RR, Schwartz-Filho HO, Novaes AB Jr, Garlet GP, Souza RA, Taba M Jr, Souza SLS, Ribeiro FJ (2009) Antimicrobial photodynamic therapy in the non-surgical treatment of aggressive periodontitis: cytokine profile in gingival crevicular fluid, preliminary results. J Periodontol 80:98–105CrossRefPubMed
45.
Metadata
Title
Adjunctive effect of antimicrobial photodynamic therapy in induced periodontal disease. Animal study with histomorphometrical, immunohistochemical, and cytokine evaluation
Authors
Paula Gabriela Faciola Pessôa de Oliveira
Adriana Maria Mariano Silveira e Souza
Arthur Belém Novaes Jr
Mário Taba Jr
Michel Reis Messora
Daniela Bazan Palioto
Márcio Fernando Moraes Grisi
Antônio Cláudio Tedesco
Sérgio Luis Scombatti de Souza
Publication date
01-09-2016
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 7/2016
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-016-1960-5

Other articles of this Issue 7/2016

Lasers in Medical Science 7/2016 Go to the issue

Original Article

Estimating the concentration of urea and creatinine in the human serum of normal and dialysis patients through Raman spectroscopy

Original Article

Pleurodesis with Thulium Cyber Laser versus talc poudrage: a comparative experimental study

Original Article

Gain-switched 311-nm Ti:Sapphire laser might be a potential treatment modality for atopic dermatitis

Original Article

Online fast Biospeckle monitoring of drug action in Trypanosoma cruzi parasites by motion history image

Original Article

Nonlinear optical microscopy for label-free detection of gastrointestinal neuroendocrine tumors

Original Article

Combination therapy for onychomycosis using a fractional 2940-nm Er:YAG laser and 5 % amorolfine lacquer