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01-04-2017 | Original Article

Adenosine A_2A receptors in the olfactory bulb suppress rapid eye movement sleep in rodents

Authors: Yi-Qun Wang, Rui Li, Dian-Ru Wang, Yoan Cherasse, Ze Zhang, Meng-Qi Zhang, Oriana Lavielle, Kristopher McEown, Serge N. Schiffmann, Alban de Kerchove d’Exaerde, Wei-Min Qu, Michael Lazarus, Zhi-Li Huang

Published in: Brain Structure and Function | Issue 3/2017

Abstract

Rapid eye movement (REM) sleep behavior disorder in humans is often accompanied by a reduced ability to smell and detect odors, and olfactory bulbectomized rats exhibit increased REM sleep, suggesting that the olfactory bulb (OB) is involved in REM-sleep regulation. However, the molecular mechanism of REM-sleep regulation by the OB is unknown. Adenosine promotes sleep and its A_2A receptors (A_2AR) are expressed in the OB. We hypothesized that A_2AR in the OB regulate REM sleep. Bilateral microinjections of the A_2AR antagonist SCH58261 into the rat OB increased REM sleep, whereas microinjections of the A_2AR agonist CGS21680 decreased REM sleep. Similar to the A_2AR antagonist, selective A_2AR knockdown by adeno-associated virus carrying short-hairpin RNA for A_2AR in the rat OB increased REM sleep. Using chemogenetics on the basis of designer receptors exclusively activated by designer drugs, we demonstrated that the inhibition of A_2AR neurons increased REM sleep, whereas the activation of these neurons decreased REM sleep. Moreover, using a conditional anterograde axonal tract-tracing approach, we found that OB A_2AR neurons innervate the piriform cortex and olfactory tubercle. These novel findings indicate that adenosine suppresses REM sleep via A_2AR in the OB of rodents.

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Title: Adenosine A2A receptors in the olfactory bulb suppress rapid eye movement sleep in rodents
Authors: Yi-Qun Wang
Rui Li
Dian-Ru Wang
Yoan Cherasse
Ze Zhang
Meng-Qi Zhang
Oriana Lavielle
Kristopher McEown
Serge N. Schiffmann
Alban de Kerchove d’Exaerde
Wei-Min Qu
Michael Lazarus
Zhi-Li Huang
Publication date: 01-04-2017
Publisher: Springer Berlin Heidelberg
Published in: Brain Structure and Function / Issue 3/2017
Print ISSN: 1863-2653
Electronic ISSN: 1863-2661
DOI: https://doi.org/10.1007/s00429-016-1281-2

At a glance: The STEP trials

Springer Medicine

Adenosine A_2A receptors in the olfactory bulb suppress rapid eye movement sleep in rodents

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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