Top

Published in:

01-04-2012 | Original Article

Addition of zinc methacrylate in dental polymers: MMP-2 inhibition and ultimate tensile strength evaluation

Authors: Sandrina Henn, Rodrigo Varella de Carvalho, Fabrício Aulo Ogliari, Ana Paula de Souza, Sergio Roberto Peres Line, Adriana Fernandes da Silva, Flávio Fernando Demarco, Adriana Etges, Evandro Piva

Published in: Clinical Oral Investigations | Issue 2/2012

Abstract

This study evaluated the effect of zinc methacrylate (ZM) on the inhibition of matrix metalloproteinase 2 (MMP-2) and the ultimate tensile strength (UTS) of an experimental polymer. Enzymes secreted from mouse gingival tissues were analyzed by gelatin zymography in buffers containing 5 mM CaCl₂ (Tris–CaCl₂) in 50 mM Tris–HCl buffer with various concentrations of ZM (0.5, 1, 2, 4, 8, and 16 mM). The matrix metalloproteinases present in the conditioned media were characterized by immunoprecipitation. The polymer UTS evaluation was performed in eight groups with various concentrations of ZM (0, 0.5, 1, 2.5, 5, 10, 20, and 30 wt.%), in a mechanical testing machine. MMP-2 (62 kDa) was detected in the zymographic assays and inhibited by ZM in all tested concentrations. UTS data were submitted to one-way ANOVA and Tukey's test (α = 0.05), and no significant differences were observed among groups, except in the polymer containing 30% ZM, presenting a significantly lower value when compared with the control group (p < 0.05). The results suggest that ZM inhibits MMP-2 expression in all concentrations tested, while small concentrations did not affect the ultimate tensile strength of the polymer. Zinc methacrylate is a metalloproteinase inhibitor that can be copolymerized with other methacrylate monomers. Yet, the addition of ZM did not affect the resin bond strength. Thus, in vivo tests should be performed to evaluate the performance of this material.

Woessner JF (1999) Matrix metalloproteinase inhibition. From the Jurassic to the third millennium. Ann N Y Acad Sci 878:388–403PubMedCrossRef

Birkedal-Hansen H, Moore WG, Bodden MK, Windsor LJ, Birkedal-Hansen B, DeCarlo A, Engler JA (1993) Matrix metalloproteinases: a review. Crit Rev Oral Biol Med 4:197–250PubMed

Raffetto JD, Ross RL, Khalil RA (2007) Matrix metalloproteinase 2-induced venous dilation via hyperpolarization and activation of K⁺ channels: relevance to varicose vein formation. J Vasc Surg 45:373–380PubMedCrossRef

Bode W, Maskos K (2003) Structural basis of the matrix metalloproteinases and their physiological inhibitors, the tissue inhibitors of metalloproteinases. Biol Chem 384:863–872PubMedCrossRef

Gusman H, Santana RB, Zehnder M (2002) Matrix metalloproteinase levels and gelatinolytic activity in clinically healthy and inflamed human dental pulps. Eur J Oral Sci 110:353–7PubMedCrossRef

Tjaderhane L, Larjava H, Sorsa T, Uitto VJ, Larmas M, Salo T (1998) The activation and function of host matrix metalloproteinases in dentin matrix breakdown in caries lesions. J Dent Res 77:1622–1629PubMedCrossRef

Martin-De Las Heras S, Valenzuela A, Overall CM (2000) The matrix metalloproteinase gelatinase A in human dentine. Arch Oral Biol 45:757–765PubMedCrossRef

Dayan D, Binderman I, Mechanic GL (1983) A preliminary study of activation of collagenase in carious human dentine matrix. Arch Oral Biol 28:185–7PubMedCrossRef

Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM, Ito S (2004) Collagen degradation by host-derived enzymes during aging. J Dent Res 83:216–221PubMedCrossRef

10.

Breschi L, Mazzoni A, Ruggeri A, Cadenaro M, Di Lenarda R, De Stefano DE (2008) Dental adhesion review: aging and stability of the bonded interface. Dent Mater 24:90–101PubMedCrossRef

11.

De Munck J, Van Meerbeek B, Yoshida Y, Inoue S, Vargas M, Suzuki K, Lambrechts P, Vanherle G (2003) Four-year water degradation of total-etch adhesives bonded to dentin. J Dent Res 82:136–140PubMedCrossRef

12.

Hebling J, Pashley DH, Tjaderhane L, Tay FR (2005) Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo. J Dent Res 84:741–746PubMedCrossRef

13.

Brackett WW, Tay FR, Brackett MG, Dib A, Sword RJ, Pashley DH (2007) The effect of chlorhexidine on dentin hybrid layers in vivo. Oper Dent 32:107–11PubMedCrossRef

14.

Carrilho MR, Carvalho RM, de Goes MF, di Hipolito V, Geraldeli S, Tay FR, Pashley DH, Tjaderhane L (2007) Chlorhexidine preserves dentin bond in vitro. J Dent Res 86:90–94PubMedCrossRef

15.

Carrilho MR, Geraldeli S, Tay F, de Goes MF, Carvalho RM, Tjaderhane L, Reis AF, Hebling J, Mazzoni A, Breschi L, Pashley D (2007) In vivo preservation of the hybrid layer by chlorhexidine. J Dent Res 86:529–533PubMedCrossRef

16.

de Souza AP, Gerlach RF, Line SR (2000) Inhibition of human gingival gelatinases (MMP-2 and MMP-9) by metal salts. Dent Mater 16:103–108PubMedCrossRef

17.

Souza AP, Gerlach RF, Line SR (2001) Inhibition of human gelatinases by metals released from dental amalgam. Biomaterials 22:2025–2030PubMedCrossRef

18.

Santos MC, de Souza AP, Gerlach RF, Trevilatto PC, Scarel-Caminaga RM, Line SR (2004) Inhibition of human pulpal gelatinases (MMP-2 and MMP-9) by zinc oxide cements. J Oral Rehabil 31:660–664PubMedCrossRef

19.

Larsen KS, Auld DS (1991) Characterization of an inhibitory metal binding site in carboxypeptidase A. Biochemistry 30:2613–8PubMedCrossRef

20.

Carvalho RV, Ogliari FA, de Souza AP, Silva AF, Petzhold CL, Line SR, Piva E, Etges A (2009) 2-Hydroxyethyl methacrylate as an inhibitor of matrix metalloproteinase-2. Eur J Oral Sci 117:64–7PubMedCrossRef

21.

de Carvalho RV, Ogliari FA, Marques MR, de Souza AP, Petzhold CL, Line SR, Piva E, Etges A (2010) Inhibition of the activity of matrix metalloproteinase 2 by triethylene glycol dimethacrylate. Clin Oral Investig. doi:10.1007/s00784-010-0443-5

22.

Gendron R, Grenier D, Sorsa T, Mayrand D (1999) Inhibition of the activities of matrix metalloproteinases 2, 8, and 9 by chlorhexidine. Clin Diagn Lab Immunol 6:437–439PubMed

23.

Nishitani Y, Yoshiyama M, Wadgaonkar B, Breschi L, Mannello F, Mazzoni A, Carvalho RM, Tjaderhane L, Tay FR, Pashley DH (2006) Activation of gelatinolytic/collagenolytic activity in dentin by self-etching adhesives. Eur J Oral Sci 114:160–166PubMedCrossRef

24.

Tay FR, Pashley DH, Loushine RJ, Weller RN, Monticelli F, Osorio R (2006) Self-etching adhesives increase collagenolytic activity in radicular dentin. J Endod 32:862–868PubMedCrossRef

Title: Addition of zinc methacrylate in dental polymers: MMP-2 inhibition and ultimate tensile strength evaluation
Authors: Sandrina Henn
Rodrigo Varella de Carvalho
Fabrício Aulo Ogliari
Ana Paula de Souza
Sergio Roberto Peres Line
Adriana Fernandes da Silva
Flávio Fernando Demarco
Adriana Etges
Evandro Piva
Publication date: 01-04-2012
Publisher: Springer-Verlag
Published in: Clinical Oral Investigations / Issue 2/2012
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-011-0551-x

At a glance: The STEP trials

Springer Medicine

Addition of zinc methacrylate in dental polymers: MMP-2 inhibition and ultimate tensile strength evaluation

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 2/2012

Cytokine production by leukocytes of Papillon–Lefèvre syndrome patients in whole blood cultures

Randomized controlled trial on fluoride varnish application for treatment of white spot lesion after fixed orthodontic treatment

Survival of atraumatic restorative treatment (ART) sealants and restorations: a meta-analysis

Oral cGVHD screening tests in the diagnosis of systemic chronic graft-versus-host disease

Fracture toughness of dental restorative materials

No evidence of HAND2 involvement in nonsyndromic cleft lip with or without cleft palate