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European Journal of Medical Research
Published in: European Journal of Medical Research 1/2023

Open Access 01-12-2023 | Acute Myeloid Leukemia | Research

Analysis of the differential expression and prognostic relationship of DEGs in AML based on TCGA database

Authors: Yue Gao, Yinnong Jia, Zhengmin Yu, Xinyu Ji, Xiaowen Liu, Lei Han, Hengdong Zhang, Baoli Zhu, Ming Xu

Published in: European Journal of Medical Research | Issue 1/2023

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Abstract

Background

Acute myeloid leukemia (AML) is a common and lethal hematological malignant hyperplastic disease originating from hematopoietic stem cells. The purpose of this study is to obtain the key differentially expressed gene (DEG) related to the survival of AML by The Cancer Genome Atlas (TCGA) database and to verify these genes by a clinical follow-up investigation, in order to identify valuable predictive and prognostic biomarkers for early diagnosis of AML and predict the survival rates.

Methods

The RNA sequencing (RNA-Seq) data and clinical information of TCGA-LAML were downloaded from the TCGA database. After that we (1) screened the survival-related DEGs by Cox regression analysis, (2) selected the cytogenetics risk-related DEGs by DESeq2 R package, and (3) filtrated the genes in the top10 pathways of up-regulated and down-regulated of Normalization Enrichment Score (NES) by Gene Set Enrichment Analysis (GSEA). Finally, we focused the intersectional genes of above three parts as the key gene of the present study. The following Multivariate.
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Metadata
Title
Analysis of the differential expression and prognostic relationship of DEGs in AML based on TCGA database
Authors
Yue Gao
Yinnong Jia
Zhengmin Yu
Xinyu Ji
Xiaowen Liu
Lei Han
Hengdong Zhang
Baoli Zhu
Ming Xu
Publication date
01-12-2023
Publisher
BioMed Central
Published in
European Journal of Medical Research / Issue 1/2023
Electronic ISSN: 2047-783X
DOI
https://doi.org/10.1186/s40001-023-01060-3

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