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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2003

Open Access 01-12-2003 | Research

Activin-A up-regulates type I activin receptor mRNA levels in human immortalized extravillous trophoblast cells.

Authors: Victor TS Chen, Chun Peng, Peter CK Leung

Published in: Reproductive Biology and Endocrinology | Issue 1/2003

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Abstract

Activin is known to play an important regulatory role in reproduction, including pregnancy. To further examine the role and signaling mechanism of activin in regulating placental function, the steady-state level of activin type I receptor (ActRI) mRNA in immortalized extravillous trophoblasts (IEVT) cells was measured using competitive PCR (cPCR). An internal standard of ActRI cDNA for cPCR was constructed for the quantification of ActRI mRNA levels in IEVT cells. ActRI mRNA levels were increased in a dose-dependent manner by activin-A with the maximal effect observed at the dose of 10 ng/ml. Time course studies revealed that activin-A had maximal effects on ActRI mRNA levels at 6 hours after treatment. The effects of activin-A on ActRI mRNA levels was blocked by follistatin, an activin binding protein, in a dose-dependent manner. In addition, inhibin-A inhibited basal, as well as activin-A-induced ActRI mRNA levels. These findings provide evidence, for the first time, that activin-A modulates ActRI mRNA levels in human trophoblast cells.
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Metadata
Title
Activin-A up-regulates type I activin receptor mRNA levels in human immortalized extravillous trophoblast cells.
Authors
Victor TS Chen
Chun Peng
Peter CK Leung
Publication date
01-12-2003
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2003
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/1477-7827-1-29

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