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01-02-2012 | 2011 SSAT Plenary Presentation

Activation of the BMP4 Pathway and Early Expression of CDX2 Characterize Non-specialized Columnar Metaplasia in a Human Model of Barrett’s Esophagus

Authors: Daniel Castillo, Sonia Puig, Mar Iglesias, Agustín Seoane, Carme de Bolós, Vicente Munitiz, Pascual Parrilla, Laura Comerma, Richard Poulsom, Kausilia K. Krishnadath, Luís Grande, Manuel Pera

Published in: Journal of Gastrointestinal Surgery | Issue 2/2012

Abstract

Background

A human model of gastroesophageal reflux disease was used to examine the contribution of a non-specialized columnar type of metaplasia (NSCM) and key molecular events (BMP4 and CDX2) in the development of Barrett’s esophagus.

Methods

Biopsies of the remnant esophagus from 18 patients undergoing esophagectomy with gastric preservation were taken at 6–36-month intervals postoperatively and examined for activation of the BMP pathway (BMP4/P-Smad 1/5/8) and CDX2 and CDX1 expression by imunohistochemistry, quantitative real-time PCR, Western blot, and in situ hybridization.

Results

A short segment (mean 15.6 mm) of NSCM was detected in 10 (56%) patients, with an increasing prevalence from 17% at 6 months to 62% at 36 months. Nuclear expression of P-Smad 1/5/8 in the squamous epithelium close to the anastomosis with strong expression in all epithelial cells of NSCM areas was found. Forty-eight (63%) biopsies with NSCM showed scattered nuclear expression of CDX2. Two cases showed isolated glands at 18, 24, and 36 months that fully expressed CDX2 and co-expressed CDX1. BMP4 mRNA and CDX2 mRNA levels were significantly greater in NSCM than in squamous epithelium.

Conclusions

BMP4 activation in NSCM and early expression of CDX2 are involved in the columnar epithelial differentiation of Barrett’s esophagus.

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Title: Activation of the BMP4 Pathway and Early Expression of CDX2 Characterize Non-specialized Columnar Metaplasia in a Human Model of Barrett’s Esophagus
Authors: Daniel Castillo
Sonia Puig
Mar Iglesias
Agustín Seoane
Carme de Bolós
Vicente Munitiz
Pascual Parrilla
Laura Comerma
Richard Poulsom
Kausilia K. Krishnadath
Luís Grande
Manuel Pera
Publication date: 01-02-2012
Publisher: Springer-Verlag
Published in: Journal of Gastrointestinal Surgery / Issue 2/2012
Print ISSN: 1091-255X
Electronic ISSN: 1873-4626
DOI: https://doi.org/10.1007/s11605-011-1758-5

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Springer Medicine

Activation of the BMP4 Pathway and Early Expression of CDX2 Characterize Non-specialized Columnar Metaplasia in a Human Model of Barrett’s Esophagus

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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