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01-08-2009 | Original Paper

Activation of peroxisome proliferator-activated receptor-γ inhibits transforming growth factor-β1 induction of connective tissue growth factor and extracellular matrix in hypertrophic scar fibroblasts in vitro

Authors: Guo-You Zhang, Tao Cheng, Ming-Hua Zheng, Cheng-Gang Yi, Hua Pan, Zhi-Jie Li, Xing-Long Chen, Qing Yu, Liang-Fu Jiang, Fei-Ya Zhou, Xiao-Yang Li, Jing-Quan Yang, Ting-Gang Chu, Wei-Yang Gao

Published in: Archives of Dermatological Research | Issue 7/2009

Abstract

Peroxisome proliferator-activated receptor-γ (PPAR-γ) ligands have been recently reported to have beneficial effects on organ fibrosis. However, their effects on extracellular matrix (ECM) turnover in hypertrophic scar fibroblasts (HSFs), and the related molecular mechanisms are unknown. HSFs were cultured and exposed to different concentration PPAR-γ ligands in the presence of transforming growth factor-β1 (TGF-β1). In growth-arrested HSFs, a PPAR-γ natural ligand (15-deoxy-D12,14-prostaglandin J2, 15d-PGJ2) and a synthetic ligand (GW7845) dose-dependently attenuated TGFβ1-induced expression of Connective tissue growth factor (CTGF), collagens and fibronectin. Furthermore, the suppression of CTGF mRNA and protein expression are relieved by pretreatment with an antagonist of PPAR-γ (GW9662). Moreover, GW7845 and 15d-PGJ2 partially inhibited the expression and phosphorylation of the TGF-β1/Smad pathway. These results suggest that in TGFβ1-stimulated HSFs, PPAR-γ ligands caused an antiproliferative effect and reduced ECM production through mechanisms that included reducing CTGF expression, and a crosstalk between PPAR-γ and Smad may be involved in the inhibitory effects of PPAR-γ ligands.

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Title: Activation of peroxisome proliferator-activated receptor-γ inhibits transforming growth factor-β1 induction of connective tissue growth factor and extracellular matrix in hypertrophic scar fibroblasts in vitro
Authors: Guo-You Zhang
Tao Cheng
Ming-Hua Zheng
Cheng-Gang Yi
Hua Pan
Zhi-Jie Li
Xing-Long Chen
Qing Yu
Liang-Fu Jiang
Fei-Ya Zhou
Xiao-Yang Li
Jing-Quan Yang
Ting-Gang Chu
Wei-Yang Gao
Publication date: 01-08-2009
Publisher: Springer-Verlag
Published in: Archives of Dermatological Research / Issue 7/2009
Print ISSN: 0340-3696
Electronic ISSN: 1432-069X
DOI: https://doi.org/10.1007/s00403-009-0959-1

At a glance: The STEP trials

Springer Medicine

Activation of peroxisome proliferator-activated receptor-γ inhibits transforming growth factor-β1 induction of connective tissue growth factor and extracellular matrix in hypertrophic scar fibroblasts in vitro

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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