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Journal of Orthopaedic Surgery and Research

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Open Access 01-12-2017 | Research article

Acceleration of tendon–bone healing of anterior cruciate ligament graft using intermittent negative pressure in rabbits

Authors: Zhengming Sun, Xiaoqing Wang, Ming Ling, Wei Wang, Yanhai Chang, Guang Yang, Xianghui Dong, Shixun Wu, Xueyuan Wu, Bo Yang, Ming Chen

Published in: Journal of Orthopaedic Surgery and Research | Issue 1/2017

Abstract

Background

The purpose of this study was to test effects of negative pressure on tendon–bone healing after reconstruction of anterior cruciate ligament (ACL) in rabbits.

Methods

Hind legs of 24 New Zealand White rabbits were randomly selected as negative pressure group and the contralateral hind legs as control. Reconstruction of the ACL was done. Joints of the negative pressure side were placed with drainage tubes connecting the micro-negative pressure aspirator. Control side was placed with ordinary drainage tubes. Drainage tubes on both sides were removed at the same time 5 days after operation. After 6 weeks, joint fluid was drawn to detect the expression levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α); at the same time, femur–ligament–tibia complex was obtained to determine tendon graft tension and to observe the histomorphology, blood vessels of the tendon–bone interface, and expression of vascular endothelial growth factor (VEGF).

Results

The maximum load breakage of tendon graft was significantly greater in the negative pressure group than in the control group (P < 0.05). Histological studies of the tendon–bone interface found that there was more new bone formation containing chondroid cells and aligned connective tissue in the negative pressure group than in the control group. Expression of VEGF was higher in the negative pressure group than in the control group (P < 0.01). Content of IL-1β and TNF-α in synovial fluid is lower in the negative pressure group than in the control group (P < 0.01).

Conclusions

Intermittent negative pressure plays an active role in tendon–bone healing and creeping substitution of ACL reconstruction in the rabbits.

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Title: Acceleration of tendon–bone healing of anterior cruciate ligament graft using intermittent negative pressure in rabbits
Authors: Zhengming Sun
Xiaoqing Wang
Ming Ling
Wei Wang
Yanhai Chang
Guang Yang
Xianghui Dong
Shixun Wu
Xueyuan Wu
Bo Yang
Ming Chen
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: Journal of Orthopaedic Surgery and Research / Issue 1/2017
Electronic ISSN: 1749-799X
DOI: https://doi.org/10.1186/s13018-017-0561-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Acceleration of tendon–bone healing of anterior cruciate ligament graft using intermittent negative pressure in rabbits

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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