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Published in: Lasers in Medical Science 3/2015

01-04-2015 | Original Article

Ablation of porcine bone tissue with an ultrashort pulsed laser (USPL) system

Authors: Christina Plötz, Florian Schelle, Christoph Bourauel, Matthias Frentzen, Jörg Meister

Published in: Lasers in Medical Science | Issue 3/2015

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Abstract

Ultrashort pulsed lasers (USPLs) represent a new generation of laser systems in the field of biophotonical applications. In terms of a pilot project, the study was carried out to evaluate the ablation parameters of bone tissue regarding the medical use of such a laser technology in dentistry. Specimens from ribs of freshly slaughtered pigs were assembled and irradiated with an USP Nd:YVO4 laser (pulse duration 8 ps at 1,064 nm with repetition rates between 50 and 500 kHz) using eligible average output powers in the range of 3.5–9 W and fluences between 1 and 2.5 J/cm2. Square-shaped cavities of 1-mm edge length in the bone compacta were created employing a scanner system. Cavities were analyzed with an optical profilometer to determine the ablated volume. Ablation rate was calculated by the ablated volume and the recorded irradiation time by the scanner software. Additionally, samples were examined histologically to investigate side effects of the surrounding tissue. Formed cavities showed a precise and sharp-edged appearance in bone compacta. Optimized ablation rate of 5.2 mm3/min without any accompanying side effects was obtained with an average output power of 9 W, a pulse repetition rate of 500 kHz, and an applied fluence of 2.5 J/cm2. Provided that the used laser system will be advanced and adjusted for clinical applications, the outcome of this study shows auspicious possibilities for the use of USPL systems in the preparation of bone tissue.
Literature
2.
go back to reference Romeo U, Del Vecchio A, Palaia G, Tenore G, Visca P, Maggiore C (2009) Bone damage induced by different cutting instruments—an in vitro study. Braz Dent J 20(2):162–168CrossRefPubMed Romeo U, Del Vecchio A, Palaia G, Tenore G, Visca P, Maggiore C (2009) Bone damage induced by different cutting instruments—an in vitro study. Braz Dent J 20(2):162–168CrossRefPubMed
3.
go back to reference Pourzarandian A, Watanabe H, Aoki A, Ichinose S, Sasaki KM, Nitta H, Ishikawa I (2004) Histological and TEM examination of early stages of bone healing after Er:YAG laser irradiation. Photomed Laser Surg 22(4):342–350. doi:10.1089/pho.2004.22.342 CrossRefPubMed Pourzarandian A, Watanabe H, Aoki A, Ichinose S, Sasaki KM, Nitta H, Ishikawa I (2004) Histological and TEM examination of early stages of bone healing after Er:YAG laser irradiation. Photomed Laser Surg 22(4):342–350. doi:10.​1089/​pho.​2004.​22.​342 CrossRefPubMed
5.
go back to reference Bengazi F, Lang NP, Canciani E, Viganò P, Velez JU, Botticelli D (2012) Osseointegration of implants with dendrimers surface characteristics installed conventionally or with Piezosurgery®. A comparative study in the dog. Clin Oral Implants Res. doi:10.1111/clr.12082 [Epub ahead of print]PubMed Bengazi F, Lang NP, Canciani E, Viganò P, Velez JU, Botticelli D (2012) Osseointegration of implants with dendrimers surface characteristics installed conventionally or with Piezosurgery®. A comparative study in the dog. Clin Oral Implants Res. doi:10.​1111/​clr.​12082 [Epub ahead of print]PubMed
8.
go back to reference Stübinger S, Biermeier K, Bächi B, Ferguson SJ, Sader R, von Rechenberg B (2010) Comparison of Er:YAG laser, piezoelectric, and drill osteotomy for dental implant site preparation: a biomechanical and histological analysis in sheep. Lasers Surg Med 42(7):652–661. doi:10.1002/lsm.20944 CrossRefPubMed Stübinger S, Biermeier K, Bächi B, Ferguson SJ, Sader R, von Rechenberg B (2010) Comparison of Er:YAG laser, piezoelectric, and drill osteotomy for dental implant site preparation: a biomechanical and histological analysis in sheep. Lasers Surg Med 42(7):652–661. doi:10.​1002/​lsm.​20944 CrossRefPubMed
11.
go back to reference Niemz MH (1996) Laser–tissue interactions: fundamentals and applications. Springer Verlag, Berlin, Heidelberg, New York Niemz MH (1996) Laser–tissue interactions: fundamentals and applications. Springer Verlag, Berlin, Heidelberg, New York
13.
14.
go back to reference Schwab B, Hagner D, Müller W, Lubatschowski H, Lenarz T, Heermann R (2004) Bone ablation using ultrashort laser pulses. A new technique for middle ear surgery. Laryngo-Rhino-Otol 83(4):219–225. doi:10.1055/s-2004-814270 [Article in German]CrossRef Schwab B, Hagner D, Müller W, Lubatschowski H, Lenarz T, Heermann R (2004) Bone ablation using ultrashort laser pulses. A new technique for middle ear surgery. Laryngo-Rhino-Otol 83(4):219–225. doi:10.​1055/​s-2004-814270 [Article in German]CrossRef
16.
go back to reference Schelle F, Polz S, Haloui H, Braun A, Dehn C, Frentzen M, Meister J (2013) Ultrashort pulsed laser (USPL) application in dentistry: basic investigations of ablation rates and thresholds on oral hard tissue and restorative materials. Lasers Med Sci. doi:10.1007/s10103-013-1315-4 [Epub ahead of print] Schelle F, Polz S, Haloui H, Braun A, Dehn C, Frentzen M, Meister J (2013) Ultrashort pulsed laser (USPL) application in dentistry: basic investigations of ablation rates and thresholds on oral hard tissue and restorative materials. Lasers Med Sci. doi:10.​1007/​s10103-013-1315-4 [Epub ahead of print]
17.
go back to reference Tukey JW (1977). Exploratory Data Analysis (Addison-Wesley Series in Behavioral Science-Quantitative Methods). Addison Wesley Pub Co Inc, Boston Tukey JW (1977). Exploratory Data Analysis (Addison-Wesley Series in Behavioral Science-Quantitative Methods). Addison Wesley Pub Co Inc, Boston
18.
go back to reference Bello-Silva MS, Martin Wehner M, Eduardo CP, Lampert F, Poprawe R, Hermans M, Esteves-Oliveira M (2012) Precise ablation of dental hard tissues with ultra-short pulsed lasers. Preliminary exploratory investigation on adequate laser parameters. Lasers Med Sci. doi:10.1007/s10103-012-1107-2 [Epub ahead of print] Bello-Silva MS, Martin Wehner M, Eduardo CP, Lampert F, Poprawe R, Hermans M, Esteves-Oliveira M (2012) Precise ablation of dental hard tissues with ultra-short pulsed lasers. Preliminary exploratory investigation on adequate laser parameters. Lasers Med Sci. doi:10.​1007/​s10103-012-1107-2 [Epub ahead of print]
20.
go back to reference Girard B, Cloutier M, Wilson DJ, Clokie CM, Miller RJ, Wilson BC (2007) Microtomographic analysis of healing of femtosecond laser bone calvarial wounds compared to mechanical instruments in mice with and without application of BMP-7. Lasers Surg Med 39(5):458–467. doi:10.1002/lsm.20493 CrossRefPubMed Girard B, Cloutier M, Wilson DJ, Clokie CM, Miller RJ, Wilson BC (2007) Microtomographic analysis of healing of femtosecond laser bone calvarial wounds compared to mechanical instruments in mice with and without application of BMP-7. Lasers Surg Med 39(5):458–467. doi:10.​1002/​lsm.​20493 CrossRefPubMed
22.
go back to reference Stübinger S, von Rechenberg B, Zeilhofer HF, Sader R, Landes C (2007) Er:YAG laser osteotomy for removal of impacted teeth: clinical comparison of two techniques. Lasers Surg Med 39(7):583–588. doi:10.1002/lsm.20528 CrossRefPubMed Stübinger S, von Rechenberg B, Zeilhofer HF, Sader R, Landes C (2007) Er:YAG laser osteotomy for removal of impacted teeth: clinical comparison of two techniques. Lasers Surg Med 39(7):583–588. doi:10.​1002/​lsm.​20528 CrossRefPubMed
23.
go back to reference Stock K, Diebolder R, Hausladen F, Wurm H, Lorenz S, Hibst R (2013) Primary investigations on the potential of a novel diode pumped Er:YAG laser system for bone surgery. In: Rechmann P, Fried D, (eds) Lasers in Dentistry XIX, Proc SPIE 8565:85656D–1–85656D–8. doi:10.1117/12.2004123. Stock K, Diebolder R, Hausladen F, Wurm H, Lorenz S, Hibst R (2013) Primary investigations on the potential of a novel diode pumped Er:YAG laser system for bone surgery. In: Rechmann P, Fried D, (eds) Lasers in Dentistry XIX, Proc SPIE 8565:85656D–1–85656D–8. doi:10.​1117/​12.​2004123.
Metadata
Title
Ablation of porcine bone tissue with an ultrashort pulsed laser (USPL) system
Authors
Christina Plötz
Florian Schelle
Christoph Bourauel
Matthias Frentzen
Jörg Meister
Publication date
01-04-2015
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 3/2015
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-014-1520-9

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