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International Journal of Hematology
Published in: International Journal of Hematology 6/2016

01-12-2016 | Original Article

A portable platform for stepwise hematopoiesis from human pluripotent stem cells within PET-reinforced collagen sponges

Authors: Yoshinori Sugimine, Akira Niwa, Hiroyuki Matsubara, Kazuki Kobayashi, Yasuhiko Tabata, Toshio Heike, Tatsutoshi Nakahata, Megumu K. Saito

Published in: International Journal of Hematology | Issue 6/2016

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Abstract

Various systems for differentiating hematopoietic cells from human pluripotent stem cells (PSCs) have been developed, although none have been fully optimized. In this report, we describe the development of a novel three-dimensional system for differentiating hematopoietic cells from PSCs using collagen sponges (CSs) reinforced with poly(ethylene terephthalate) fibers as a scaffold. PSCs seeded onto CSs were differentiated in a stepwise manner with appropriate cytokines under serum-free and feeder-free conditions. This process yielded several lineages of floating hematopoietic cells repeatedly for more than 1 month. On immunohistochemical staining, we detected CD34+ cells and CD45+ cells in the surface and cavities of the CS. Taking advantage of the portability of this system, we were able to culture multiple CSs together floating in medium, making it possible to harvest large numbers of hematopoietic cells repeatedly. Given these findings, we suggest that this novel three-dimensional culture system may be useful in the large-scale culture of PSC-derived hematopoietic cells.
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Metadata
Title
A portable platform for stepwise hematopoiesis from human pluripotent stem cells within PET-reinforced collagen sponges
Authors
Yoshinori Sugimine
Akira Niwa
Hiroyuki Matsubara
Kazuki Kobayashi
Yasuhiko Tabata
Toshio Heike
Tatsutoshi Nakahata
Megumu K. Saito
Publication date
01-12-2016
Publisher
Springer Japan
Published in
International Journal of Hematology / Issue 6/2016
Print ISSN: 0925-5710
Electronic ISSN: 1865-3774
DOI
https://doi.org/10.1007/s12185-016-2088-x

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