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Journal of Assisted Reproduction and Genetics

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01-09-2010 | Technical Innovations

A novel method for detection of chromosomal integrity in cryopreserved livestock spermatozoa using artificially fused mouse oocytes

Authors: Hiroyuki Watanabe, Hiroshi Suzuki, Hiroyuki Tateno, Yutaka Fukui

Published in: Journal of Assisted Reproduction and Genetics | Issue 9-10/2010

Abstract

Purpose

The aim of the present study was to investigate the effect of mouse oocyte volume on the efficiency of chromosomal analysis in livestock spermatozoa.

Methods

Oocytes were injected with bull, ram, boar and dog sperm heads, and then fused with enucleated mouse oocytes.

Results

The increment of oocyte volume increased the rates of morphologically normal oocytes after sperm injection, which induced much higher rates of overall chromosome detection in bull, ram and dog spermatozoa. The recipient oocyte volume did not affect the chromosomal integrity. Furthermore, in bull, the chromosomal integrity detected by fused mouse oocytes was similar to that derived from a homologous system. On the other hand, chromosomal plates of boar spermatozoa could not be detected despite the use of fused oocytes.

Conclusion

These data indicate that fused mouse oocytes improved the efficiency of chromosome detection in bull, ram and dog spermatozoa.

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Title: A novel method for detection of chromosomal integrity in cryopreserved livestock spermatozoa using artificially fused mouse oocytes
Authors: Hiroyuki Watanabe
Hiroshi Suzuki
Hiroyuki Tateno
Yutaka Fukui
Publication date: 01-09-2010
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 9-10/2010
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-010-9445-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

A novel method for detection of chromosomal integrity in cryopreserved livestock spermatozoa using artificially fused mouse oocytes

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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