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Published in: Archives of Virology 10/2012

01-10-2012 | Annotated Sequence Record

A novel mastrevirus infecting wheat in India

Authors: Jitendra Kumar, Sudhir P. Singh, Jitesh Kumar, Rakesh Tuli

Published in: Archives of Virology | Issue 10/2012

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Abstract

A new mastrevirus (family Geminivridae) infecting wheat in India was detected by rolling-circle amplification (RCA). The complete nucleotide sequence of the virus was determined to be 2783 bp long. Analysis of the nucleotide sequence revealed identity and a genome organisation typical of a mastrevirus. An identical virus was detected in the candidate insect vector (leafhopper) collected from the field. Agroinoculation of young wheat plants with an infectious clone of the virus resulted in dwarfing of plants, identical to what was observed in the field, confirming that this novel virus was the causative agent of the disease. Considering the low degree of sequence identity to any known mastrevirus, the virus described here is suggested to be a member of a new species. Based on symptoms, we propose the name “wheat dwarf India virus”.
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Literature
1.
go back to reference Khetarpal RK, Kumar J, Beuve M, Parakh DB, Nath R (1994) Outbreak of MAV-type barley yellow dwarf virus on wheat in the Garhwal Hills in India. Plant Pathol 43:415–416CrossRef Khetarpal RK, Kumar J, Beuve M, Parakh DB, Nath R (1994) Outbreak of MAV-type barley yellow dwarf virus on wheat in the Garhwal Hills in India. Plant Pathol 43:415–416CrossRef
2.
go back to reference Deb M, Anderson JM (2008) Development of a multiplexed PCR detection method for Barley and Cereal yellow dwarf viruses, Wheat spindle streak virus, Wheat streak mosaic virus and Soil-borne wheat mosaic virus. J Virol Methods 148:17–24PubMedCrossRef Deb M, Anderson JM (2008) Development of a multiplexed PCR detection method for Barley and Cereal yellow dwarf viruses, Wheat spindle streak virus, Wheat streak mosaic virus and Soil-borne wheat mosaic virus. J Virol Methods 148:17–24PubMedCrossRef
3.
go back to reference Robertson NL, French R, Gray SM (1991) Use of group-specific primers and the polymerase chain reaction for the detection and identification of luteoviruses. J Gen Virol 72:1473–1477PubMedCrossRef Robertson NL, French R, Gray SM (1991) Use of group-specific primers and the polymerase chain reaction for the detection and identification of luteoviruses. J Gen Virol 72:1473–1477PubMedCrossRef
4.
go back to reference Behjatnia SAA, Afsharifar AR, Tahan V, Motlagh MHA, Gandomani OE, Niazi A, Izadpanah K (2011) Widespread occurrence and molecular characterization of Wheat dwarf virus in Iran. Australas Plant Pathol 40:12–19CrossRef Behjatnia SAA, Afsharifar AR, Tahan V, Motlagh MHA, Gandomani OE, Niazi A, Izadpanah K (2011) Widespread occurrence and molecular characterization of Wheat dwarf virus in Iran. Australas Plant Pathol 40:12–19CrossRef
5.
go back to reference Lefeuvre P, Lett J-M, Varsani A, Martin DP (2009) Widely conserved recombination patterns amongst single stranded DNA viruses. J Virol 83:2697–2707PubMedCrossRef Lefeuvre P, Lett J-M, Varsani A, Martin DP (2009) Widely conserved recombination patterns amongst single stranded DNA viruses. J Virol 83:2697–2707PubMedCrossRef
6.
go back to reference Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P (2010) RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics 26(19):2462–2463PubMedCrossRef Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P (2010) RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics 26(19):2462–2463PubMedCrossRef
7.
go back to reference Lawry R, Martin DP, Shepherd DN, Antwerpen T, Varsani A (2009) A novel sugarcane-infecting mastrevirus from South Africa. Arch Virol 154:1699–1703PubMedCrossRef Lawry R, Martin DP, Shepherd DN, Antwerpen T, Varsani A (2009) A novel sugarcane-infecting mastrevirus from South Africa. Arch Virol 154:1699–1703PubMedCrossRef
8.
go back to reference Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X (2008) Geminivirus strain demarcation and nomenclature. Arch Virol 153:783–821PubMedCrossRef Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X (2008) Geminivirus strain demarcation and nomenclature. Arch Virol 153:783–821PubMedCrossRef
9.
go back to reference Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599PubMedCrossRef Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599PubMedCrossRef
Metadata
Title
A novel mastrevirus infecting wheat in India
Authors
Jitendra Kumar
Sudhir P. Singh
Jitesh Kumar
Rakesh Tuli
Publication date
01-10-2012
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 10/2012
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-012-1359-y

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