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BMC Cancer
Published in: BMC Cancer 1/2015

Open Access 01-12-2015 | Research article

A novel human leiomyoma tissue derived matrix for cell culture studies

Authors: Tuula Salo, Meeri Sutinen, Ehsanul Hoque Apu, Elias Sundquist, Nilva K. Cervigne, Carine Ervolino de Oliveira, Saad Ullah Akram, Steffen Ohlmeier, Fumi Suomi, Lauri Eklund, Pirjo Juusela, Pirjo Åström, Carolina Cavalcante Bitu, Markku Santala, Kalle Savolainen, Johanna Korvala, Adriana Franco Paes Leme, Ricardo D. Coletta

Published in: BMC Cancer | Issue 1/2015

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Abstract

Background

The composition of the matrix molecules is important in in vitro cell culture experiments of e.g. human cancer invasion and vessel formation. Currently, the mouse Engelbreth-Holm-Swarm (EHS) sarcoma -derived products, such as Matrigel®, are the most commonly used tumor microenvironment (TME) mimicking matrices for experimental studies. However, since Matrigel® is non-human in origin, its molecular composition does not accurately simulate human TME. We have previously described a solid 3D organotypic myoma disc invasion assay, which is derived from human uterus benign leiomyoma tumor. Here, we describe the preparation and analyses of a processed, gelatinous leiomyoma matrix, named Myogel.

Methods

A total protein extract, Myogel, was formulated from myoma. The protein contents of Myogel were characterized and its composition and properties compared with a commercial mouse Matrigel®. Myogel was tested and compared to Matrigel® in human cell adhesion, migration, invasion, colony formation, spheroid culture and vessel formation experiments, as well as in a 3D hanging drop video image analysis.

Results

We demonstrated that only 34 % of Myogel’s molecular content was similar to Matrigel®. All test results showed that Myogel was comparable with Matrigel®, and when mixed with low-melting agarose (Myogel-LMA) it was superior to Matrigel® in in vitro Transwell® invasion and capillary formation assays.

Conclusions

In conclusion, we have developed a novel Myogel TME matrix, which is recommended for in vitro human cell culture experiments since it closely mimics the human tumor microenvironment of solid cancers.
Appendix
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Metadata
Title
A novel human leiomyoma tissue derived matrix for cell culture studies
Authors
Tuula Salo
Meeri Sutinen
Ehsanul Hoque Apu
Elias Sundquist
Nilva K. Cervigne
Carine Ervolino de Oliveira
Saad Ullah Akram
Steffen Ohlmeier
Fumi Suomi
Lauri Eklund
Pirjo Juusela
Pirjo Åström
Carolina Cavalcante Bitu
Markku Santala
Kalle Savolainen
Johanna Korvala
Adriana Franco Paes Leme
Ricardo D. Coletta
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2015
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/s12885-015-1944-z

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