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01-10-2014 | Research Article

A novel FGF2 antagonist peptide P8 with potent antiproliferation activity

Authors: Lei Fan, Hang Xie, Lingzi Chen, Hui Ye, Shilong Ying, Cong Wang, Xiaoping Wu, Wulan Li, Jianzhang Wu, Guang Liang, Xiaokun Li

Published in: Tumor Biology | Issue 10/2014

Abstract

Some fibroblast growth factors (FGFs) play a critical role in tumorigenesis and progression. Among them, FGF2 was highly expressed in some tumors, and antagonists binding to FGF2 can suppress the growth of tumor cells. Therefore, FGF2 has been considered as an important target in cancer therapy. In this study, we identified a novel FGF2-binding short peptide (P8, PLLQATAGGGS-NH₂) using phage display technology and alanine scanning. The P8 peptide suppressed FGF2-induced proliferation with no cytotoxic effect on cells, arrested the cycle at the G0/G1 phase in B16-F10 cells, and downregulated the activation of fibroblast growth factor receptor substrate 2α (FRS2α)/ERK cascade in B16-F10, NIH-H460, and SGC-7901 cells. Besides, P8 peptide can also inhibit the phosphorylation of FRS2α stimulated by FGF1 and KGF2. These implied that P8 peptide may develop as a multi-target antagonist peptide contributing to tumor treatment.

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Title: A novel FGF2 antagonist peptide P8 with potent antiproliferation activity
Authors: Lei Fan
Hang Xie
Lingzi Chen
Hui Ye
Shilong Ying
Cong Wang
Xiaoping Wu
Wulan Li
Jianzhang Wu
Guang Liang
Xiaokun Li
Publication date: 01-10-2014
Publisher: Springer Netherlands
Published in: Tumor Biology / Issue 10/2014
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI: https://doi.org/10.1007/s13277-014-2356-4

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