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Tumor Biology
Published in: Tumor Biology 7/2016

01-07-2016 | Original Article

A novel dual-targeted ultrasound contrast agent provides improvement of gene delivery efficiency in vitro

Authors: Jinfeng Xu, Xinxin Zeng, Yingying Liu, Hui Luo, Zhanghong Wei, Huiyu Liu, Yuli Zhou, Hairong Zheng, Jie Zhou, Guanghong Tan, Fei Yan

Published in: Tumor Biology | Issue 7/2016

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Abstract

Ultrasound-targeted microbubble destruction (UTMD) has become a novel gene/drug delivery method in cancer therapeutic application. However, the gene transfection efficiency mediated by UTMD is still unsatisfactory. Here, we introduced iRGD/CCR2 dual-targeted cationic microbubbles (MBiRGD/CCR2) which was modified with PEI-600 and coated with iRGD peptides and anti-CCR-2 antibodies. It showed that MBiRGD/CCR2 had a 25.83 ± 1.57 mV surface zeta potential and good stability. The experiments in vitro showed MBiRGD/CCR2 had higher binding efficiency with both bEnd.3 cells and MCF-7 cells than that of iRGD or CCR2 single-targeted cationic microbubbles (MBiRGD or MBCCR2) (P < 0.05 for both). Agarose gel electrophoresis assay showed that MBiRGD/CCR2 can effectively load pGPU6/GFP/Neo-shAKT2 plasmid DNA. Compared with the plain MBs (MBcontrol) or single-targeted cationic MBs including MBiRGD and MBCCR2 (P < 0.05 for all), the dual-targeted cationic MBiRGD/CCR2 groups had higher gene transfection efficiency under US exposure. It showed that the dual-targeted cationic MBiRGD/CCR2 has a potential value to be used as an ultrasound imaging probe for ultrasound image-guided tumor gene therapy.
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Metadata
Title
A novel dual-targeted ultrasound contrast agent provides improvement of gene delivery efficiency in vitro
Authors
Jinfeng Xu
Xinxin Zeng
Yingying Liu
Hui Luo
Zhanghong Wei
Huiyu Liu
Yuli Zhou
Hairong Zheng
Jie Zhou
Guanghong Tan
Fei Yan
Publication date
01-07-2016
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 7/2016
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-015-4681-7

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