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01-08-2018 | Original Article

A novel compound-primed multiplex ARMS-PCR (CPMAP) for simultaneous detection of common PAH gene mutations

Authors: Maryam Shaykholeslam Esfahani, Ehsan Shaykholeslam Esfahani, Sadeq Vallian

Published in: Metabolic Brain Disease | Issue 4/2018

Abstract

In this study, we introduce a novel compound-primed multiplex ARMS PCR (CPMAP) for simultaneous detection of common PAH gene mutations. This approach was used successfully for simultaneous identification of six most common PAH gene mutations in 137 phenylketonuria patients in the Iranian population. A total of six normal and six mutant allele-specific primers and 4 common primers containing a tag sequence of 12 base pair at the 5ˊ-end were designed and used in two separate optimized multiplex ARMS reactions followed by hot-start PCR. The products were separated and visualized on 3% agarose gel. The CPMAP genotyping data were completely in accordance with the direct sequencing results. The CPMAP suggests a reliable, economical and rapid method for simultaneous detection of PAH point mutations using conventional PCR, which could be applied for diagnosis of other gene mutations.

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Title: A novel compound-primed multiplex ARMS-PCR (CPMAP) for simultaneous detection of common PAH gene mutations
Authors: Maryam Shaykholeslam Esfahani
Ehsan Shaykholeslam Esfahani
Sadeq Vallian
Publication date: 01-08-2018
Publisher: Springer US
Published in: Metabolic Brain Disease / Issue 4/2018
Print ISSN: 0885-7490
Electronic ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-018-0210-x

At a glance: The STEP trials

Springer Medicine

A novel compound-primed multiplex ARMS-PCR (CPMAP) for simultaneous detection of common PAH gene mutations

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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