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Graefe's Archive for Clinical and Experimental Ophthalmology
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology 9/2005

01-09-2005 | Laboratory Investigation

A Ni2+-sensitive component of the ERG b-wave from the isolated bovine retina is related to E-type voltage-gated Ca2+ channels

Authors: Matthias Lüke, Margit Henry, Thea Lingohr, Mehran Maghsoodian, Jürgen Hescheler, Marco Weiergräber, Werner Sickel, Toni Schneider

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 9/2005

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Abstract

Background

Voltage-dependent Ca2+ channels trigger and control important cellular processes like neurotransmitter release and secretion, long-term potentiation, and gene expression in excitable cells. During retinal signal perception and processing, presynaptic Ca2+ channels facilitate neurotransmitter release in photoreceptors and bipolar neurons, at nonspiking synapses which generate graded potentials.

Methods

The nature of voltage-gated Ca2+ channels involved in retinal signal transduction is investigated in the present report by recording the electroretinogram (ERG) from the isolated and perfused bovine retina. Transcripts of the E/R- and T-type Ca2+ channels are detected by RT-PCR.

Results

Using the Ca2+ channel antagonists (±)-isradipine, NiCl2, mibefradil, and SNX-482 results in either stimulatory or inhibitory effects on the ERG b-wave amplitude. On the transcript level, mRNA is detected for the E/R-type and a T-type voltage-gated Ca2+ channel containing Cav2.3 and Cav3.1 as ion-conducting subunits, respectively.

Conclusion

Blocking of the E/R-type Ca2+ channels by NiCl2 (10 μM) and SNX-482 (30 nM) contributes to the stimulatory effect, whereas antagonism of T-type as well as L-type Ca2+ channels meditates the inhibitory action on the b-wave amplitude. Thus, a novel function for E/R-type voltage-gated Ca2+ channels is probably associated with the visual signal transduction in the mammalian retina.
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Metadata
Title
A Ni2+-sensitive component of the ERG b-wave from the isolated bovine retina is related to E-type voltage-gated Ca2+ channels
Authors
Matthias Lüke
Margit Henry
Thea Lingohr
Mehran Maghsoodian
Jürgen Hescheler
Marco Weiergräber
Werner Sickel
Toni Schneider
Publication date
01-09-2005
Publisher
Springer-Verlag
Published in
Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 9/2005
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI
https://doi.org/10.1007/s00417-005-1145-6

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