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Journal of Assisted Reproduction and Genetics

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01-08-2015 | Fertility Preservation

A new method for evaluating the risk of transferring leukemic cells with transplanted cryopreserved ovarian tissue

Authors: Tristan Zver, Magalie Alvergnas-Vieille, Francine Garnache-Ottou, Christophe Roux, Clotilde Amiot

Published in: Journal of Assisted Reproduction and Genetics | Issue 8/2015

Abstract

Purpose

This study aimed to develop a method to detect ovarian residual disease by multicolor flow cytometry in acute leukemia patients.

Methods

We designed an experimental model consisting in adding acute leukemia cells to a cell suspension obtained from healthy ovarian cortex. Leukemic cell detection within the ovarian cell suspension required the development of a specific myeloid antibody panel different from that commonly used for minimal residual disease (MRD) monitoring in bone marrow. The method was then used to detect ovarian residual disease in 11 acute leukemia patients.

Results

Multicolor flow cytometry is able to evaluate the presence of viable leukemic cells in the ovarian cortex with good specificity and robust sensitivity of 10−4. We observed a good correlation between multicolor flow cytometry and quantitative polymerase chain reaction results. Ovarian residual disease detection by multicolor flow cytometry was positive in 3 out of 11 acute leukemia patients.

Conclusion

Multicolor flow cytometry can potentially be applied to ovarian tissue from all acute leukemia patients and is essential to evaluate the risk of cancer re-seeding before autograft of ovarian tissue in case of acute leukemia.

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Title: A new method for evaluating the risk of transferring leukemic cells with transplanted cryopreserved ovarian tissue
Authors: Tristan Zver
Magalie Alvergnas-Vieille
Francine Garnache-Ottou
Christophe Roux
Clotilde Amiot
Publication date: 01-08-2015
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 8/2015
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-015-0512-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

A new method for evaluating the risk of transferring leukemic cells with transplanted cryopreserved ovarian tissue

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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