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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 5/2017

01-05-2017 | Gamete Biology

A new media without animal component for sperm cryopreservation: motility and various attributes affecting paternal contribution of sperm

Authors: Akansha Tiwari, Merih Tekcan, Leyla Sati, William Murk, Jill Stronk, Gabor Huszar

Published in: Journal of Assisted Reproduction and Genetics | Issue 5/2017

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Abstract

Purpose

Our aim was the development of a safe sperm cryopreservation New Media (NM), composed of consistent and reproducible components devoid of any animal origin, and evaluation of NM in terms of its effect on sperm structure and function as compared to regularly used yolk media (TYM) (Irvine Scientific).

Methods

We evaluated patient semen samples and cryopreserved them in duplicates in either NM or TYM. The samples were cryopreserved for either a short term of 1 week or long term of 1 month prior to thawing. The parameters investigated include sperm motility via computer-assisted semen analysis (CASA), sperm concentration, and sperm biomarkers that promote paternal contribution of spermatozoa to fertilization including hyaluronic acid binding, chromatin maturity, apoptotic markers, cytoplasmic retention, and sperm DNA integrity.

Results

As compared to TYM, NM was equally capable of sperm cryopreservation with both short-term and long-term storage in media, and after freeze-thaw and gradient processing of sperm. HA binding of sperm was comparable post thaw in both NM and yolk media. There are also no differences observed between the samples cryopreserved in NM or TYM in terms of their aniline blue staining, CK immunocytochemistry, caspase 3 immunostaining, or DNA nick translation.

Conclusions

NM has the advantage of being xeno-free, yet in preservation of sperm motility and other sperm attributes, the NM is as effective as the TYM.
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Metadata
Title
A new media without animal component for sperm cryopreservation: motility and various attributes affecting paternal contribution of sperm
Authors
Akansha Tiwari
Merih Tekcan
Leyla Sati
William Murk
Jill Stronk
Gabor Huszar
Publication date
01-05-2017
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 5/2017
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-017-0888-4

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