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Open Access 01-03-2015 | Original Article

A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks

Authors: Eric Erbs, Lauren Faget, Gregory Scherrer, Audrey Matifas, Dominique Filliol, Jean-Luc Vonesch, Marc Koch, Pascal Kessler, Didier Hentsch, Marie-Christine Birling, Manoussos Koutsourakis, Laurent Vasseur, Pierre Veinante, Brigitte L. Kieffer, Dominique Massotte

Published in: Brain Structure and Function | Issue 2/2015

Abstract

Opioid receptors are G protein-coupled receptors (GPCRs) that modulate brain function at all levels of neural integration, including autonomic, sensory, emotional and cognitive processing. Mu (MOR) and delta (DOR) opioid receptors functionally interact in vivo, but whether interactions occur at circuitry, cellular or molecular levels remains unsolved. To challenge the hypothesis of MOR/DOR heteromerization in the brain, we generated redMOR/greenDOR double knock-in mice and report dual receptor mapping throughout the nervous system. Data are organized as an interactive database offering an opioid receptor atlas with concomitant MOR/DOR visualization at subcellular resolution, accessible online. We also provide co-immunoprecipitation-based evidence for receptor heteromerization in these mice. In the forebrain, MOR and DOR are mainly detected in separate neurons, suggesting system-level interactions in high-order processing. In contrast, neuronal co-localization is detected in subcortical networks essential for survival involved in eating and sexual behaviors or perception and response to aversive stimuli. In addition, potential MOR/DOR intracellular interactions within the nociceptive pathway offer novel therapeutic perspectives.

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Title: A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks
Authors: Eric Erbs
Lauren Faget
Gregory Scherrer
Audrey Matifas
Dominique Filliol
Jean-Luc Vonesch
Marc Koch
Pascal Kessler
Didier Hentsch
Marie-Christine Birling
Manoussos Koutsourakis
Laurent Vasseur
Pierre Veinante
Brigitte L. Kieffer
Dominique Massotte
Publication date: 01-03-2015
Publisher: Springer Berlin Heidelberg
Published in: Brain Structure and Function / Issue 2/2015
Print ISSN: 1863-2653
Electronic ISSN: 1863-2661
DOI: https://doi.org/10.1007/s00429-014-0717-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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