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International Journal of Colorectal Disease

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01-06-2008 | Original Article

A local imbalance between MMP and TIMP may have an implication on the severity and course of appendicitis

Authors: Anna Solberg, Lena Holmdahl, Peter Falk, Ingrid Palmgren, Marie-Louise Ivarsson

Published in: International Journal of Colorectal Disease | Issue 6/2008

Abstract

Background

Matrix metalloproteinases (MMPs) and the tissue inhibitors of MMPs (TIMPs) have been demonstrated to be involved in inflammatory conditions in the intestine. The purpose of this study was to investigate whether the alterations of the MMP/TIMP balance might reflect the course of the inflammatory process in acute appendicitis and if the expression and localisation of MMPs and TIMP is variable in the various clinical manifestations of appendicitis.

Materials and methods

The study comprises 40 patients (26 men and 14 women) having emergency appendectomy and a control group constituting of 10 patients (5 men and 5 women) having a hemicolectomy for other reasons. MMP and TIMP expressions were assessed and compared in tissue specimens from phlegmonous (n = 15), gangrenous (n = 7), perforated appendicitis (n = 11) and controls with noninflamed appendices (n = 10) by means of enzyme-linked immunosorbent assay technique. Localisation of the enzymes was performed by immunohistochemistry.

Results

MMP-1 was significantly higher in gangrenous and perforated appendicitis compared with phlegmonous appendicitis and controls (p < 0.05) while MMP-2 was significantly lower in gangrenous appendicitis compared with phlegmonous appendicitis and controls. MMP-2 was also lower in perforated appendicitis when compared with controls (p < 0.01). Elevated expression of MMP-9 was demonstrated in all groups of appendicitis compared with the controls (p < 0.001).

Conclusions

MMP-9 is the most abundantly expressed MMP of those investigated in inflamed appendix. We postulate that a local imbalance between MMP-9 and TIMP-1 may trigger a perforation. These results suggest that MMPs might be useful as biomarkers of appendices prone to perforation.

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Title: A local imbalance between MMP and TIMP may have an implication on the severity and course of appendicitis
Authors: Anna Solberg
Lena Holmdahl
Peter Falk
Ingrid Palmgren
Marie-Louise Ivarsson
Publication date: 01-06-2008
Publisher: Springer-Verlag
Published in: International Journal of Colorectal Disease / Issue 6/2008
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI: https://doi.org/10.1007/s00384-008-0452-x

Keynote webinar | Spotlight on medication adherence

Springer Medicine

A local imbalance between MMP and TIMP may have an implication on the severity and course of appendicitis

Abstract

Background

Materials and methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Materials and methods

Results

Conclusions

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